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To solve the mining safety and health needs of the future, access to, and sharing of, timely and relevant information will be crucial. The next generation of the Web will provide tools (RDF, OWL, SPARQL, etc) to define and link content for more effective discovery, integration, and reuse across applications and organisations. A recent Gartner Research Report (Finding and exploiting value in Semantic Web technologies on the web, May 2007) predicts these technologies will begin to coalesce in ten years. Implementing this Semantic Web requires structured web content described via metadata (information about information). If an industry or enterprise bases its metadata on a common vocabulary, it becomes possible to share and reuse information. The National Institute for Occupational Safety and HealthÆs (NIOSH) Mining web site provides access to more than 1500 NIOSH-developed publications, products, and topic pages on issues as diverse as ground control, respiratory diseases, and mine emergency management. As the site has grown, locating content has become increasingly difficult. To improve the searching process, a panel of internal experts representing all major research programs was established to develop a controlled vocabulary for indexing that content. The outcome is the NIOSH Mining Safety and Health Thesaurus, a faceted, hierarchical thesaurus containing hundreds of mining safety and health terms organised into 20 facets (eg diseases, injuries and disorders; hazardous events and exposures, equipment, engineering and administrative controls). One immediate goal of this project is to reduce the complexity of locating information on the NIOSH Mining web site by implementing metadata and faceted information retrieval techniques. Another goal is to stimulate discussion in the international mining community regarding developing metadata standards so the industry is ready to adopt future Semantic Web technologies to improve access to mining safety and health information.

Jan 1, 2008

By Andréa Lagacé

"The milling of sulphide ores inherently generates “thiosalts”, a series of partially oxidized sulphur oxyanions (S2O3 2-, S3O6 2-, S4O6 2- etc.), which make control of effluent treatment systems difficult because their natural oxidation produces acid with the potential to lower the pH within the treatment system and/or receiving environment.At the Xstrata Copper Kidd Metallurgical Site, thiosalt oxidation in the Tailings Management Area continues after buffer storage Ponds A and C where lime is added to precipitate metals and elevate the pH to within the required range for discharge. If the additional acidity generated during the continued process of thiosalt oxidation is not neutralized there is a risk that the final effluent will be non-compliant with regulated standards.To mitigate this risk, in 2007 a full scale pilot trial was conducted with hydrogen peroxide in order to force the complete oxidation of thiosalts prior to the lime treatment stations. This method of chemical oxidation had been shown to be effective at Xstrata Zinc’s Brunswick Mine. The pilot results indicated that a maximum of 50% of the stoichiometric quantity of hydrogen peroxide is required to reduce the thiosalt concentration in the No. 2 thickener overflow stream (feed to buffer storage Ponds A and C) to less than detection (10 mg/L); however, treatment was only effective in the presence of a 20 mg/L iron (Fe) catalyst. Since the hydrogen peroxide addition point at the No. 2 tailings thickener overflow typically contains <1 mg/L total Fe, a second reagent, ferric sulphate, was used to increase the total Fe concentration to the required level. This paper describes the set-up and results of the pilot trial which were used in the design of the full-scale plant.INTRODUCTIONTailings Management Area OverviewXstrata Copper’s Kidd Metallurgical Site, located in Timmins, Ontario, is an integrated metal refining facility producing copper, zinc, cadmium, indium, liquid sulfur dioxide, and sulfuric acid from third party custom feeds, and mineral concentrates recovered from its 7,800 tonne per day mill."

Jan 1, 2010

By Kazuhide Nakaniwa

In this research, we devised a measurement method using total stations (TSs) which have ?the Baum? which is a concentric circle reticle, as substitute for crosshairs. The Baum owes its name to the similarity between the concentric circles and trees (Baum in German). By setting one circle on both of edges of cylinders, operators can set their sights on points on the center line of the cylinders, and also can measure positional coordinates of the points. Also, by measuring two different points on the center line, operators can measure the position and tilt of the axes of the cylinders. Furthermore, we developed a pile driving navigation system. Foundation piles are used for various structures, and their forms are generally cylindrical. Especially, although it is difficult to drive tilted pile foundations, their usage are expected to expand due to their high earthquake resistance. Yet, a method to accurately measure the tilts of piles has not been established. However, we proved that operators can obtain adequate accuracy to control foundation piles through the measurement experiment using this system. Using this system, the information of the pile is measured using a TS, transferred to a PDA, and displayed from the operator?s view of the piling machine.

Aug 1, 2013

By H Sichani-Teimouri, B N. Noller, J C. Ng, R N. Alsaadi, P L. Breuer, S Shini

Cyanide has been used for over a century worldwide for gold extraction. However, the presence of several metals and metalloids in ore requires greater amount of cyanide for an efficient gold recovery due to their solubilisation in conjunction with gold. The extraction residues are discharged into tailing storage facilities (TSFs) and may cause bird mortality if there is exposure to cyanide-bearing wastewater exceeding the toxic threshold. The toxicity arises from free cyanide released from weak acid dissociable (WAD) cyanide complexes. The International Cyanide Management Code (ICMC) has deemed 50 mg/L of WAD cyanide released into TSFs to be safe for wildlife protection. However, this concentration is based on observations of an apparent lack of toxicity rather than a toxicological approach. The National Industrial Chemicals Notification and Assessment Scheme (NICNAS) identified in 2010 that there is a lack of toxicity data to properly assess the environmental significance of cyanide levels at gold mines. Lack of knowledge for the toxicity of free cyanide and metal cyanide complexes to wildlife led to consider alternative direct assessment techniques for designating safe levels. Whilst toxicity may be assessed by using young chickens with animal ethics approval, the traditional bioassay approach to assess cyanide toxicity is impractical for gold mine TSFs. Bioassays using algal species such as Euglena gracilis, a unicellular alga, are widely used for ecotoxicological studies. A unique feature is that the Euglena gracilis Z cell has plant-like while SMZ cell has animal-like characteristics. The aims of this project are to evaluate the suitability of Euglena gracilis Z and SMZ strains to assess the toxicity of free cyanide and both strong (cobalt) and weak (zinc, copper and nickel) acid dissociable metal-cyanide complexes that may be present in TSFs.CITATION:Noller, B N, Ng, J C, Shini, S, Breuer, P L, Sichani-Teimouri, H and Alsaadi, R N, 2014. Development of a toxicity approach to evaluate free cyanide and metal cyanide complexes in waters associated with gold and other mining, in Proceedings Life-of-Mine 2014 , pp 573–590 (The Australasian Institute of Mining and Metallurgy: Melbourne).

Jul 16, 2014

By A. Bouchard

"Based on laboratory testwork, a two-stage cleaner / re-cleaner circuit with feed regrind was proposed to replace an existing single-stage middling circuit treating a scavenger concentrate at Inco’s Clarabelle mill. In order to effectively transfer the technology and implement the new flowsheet in the plant, an extensive on-site pilot plant campaign was conducted. After demonstrating the improved metallurgy, the two-stage middlings circuit was successfully implemented at the operation.The present paper describes the on-site pilot plant testwork and the plant metallurgical improvement after implementation of the new middlings circuit. The addition of TETA / Na2S03 greatly enhanced the pentlandite selectivity against pyrrhotite and the two-stage cleaning considerably improved the pentlandite selectivity against rock minerals. The improvement was immediate after the installation of the new middlings circuit. Size by size recovery analysis indicated that a finer regrind would further improve the metallurgy of the new two-stage cleanerhe-cleaner circuit and those modifications are in progress at the mill.INTRODUCTIONThe milling operation at Inco’s Clarabelle mill has been described elsewhere (Kerr and Kipkie, 1985; Agar, 1991). While maximizing the recoveries of chalcopyrite (Cp) and pentlandite (Pn), continuous effort has been directed toward improving pyrrhotite (Po) and rock minerals (Rk) rejection. Inco discovered diethylene triamine (DETA) and triethylene triamine (TETA) (Marticorena, et al., 1994) for pyrrhotite rejection. Falconbridge further improved pyrrhotite rejection using DETA and SO2 (Kelebek, et al., 1995). No special technologies are used in the rejection of rock minerals."

Jan 1, 2001

By Manqiu Xu, Glen Robertson, Steve Wilson, Pete Quinn

"The middlings circuit, once called the pyrrhotite rejection circuit, at Inco’s Clarabelle mill was a single flotation stage. It used to process a combined feed consisting of the main flotation scavenger concentrate and the non-magnetic portion of the magnetics flotation circuit tails. The typical circuit metallurgy was 55% nickel recovery at 6.5% Cu+Ni concentrate grade. In the past few years, a research project with the objective of improving the circuit metallurgy was initiated between Inco’s research laboratory and the Mill’s Process Technology group. This project has gone through three steps: a) initial laboratory studies, b) on-site pilot plant investigation, and finally c) full plant implementation.The present paper describes the initial laboratory test work. The development of a two-stage circuit consisting of separate rock and pyrrhotite rejection stages to replace the simultaneous rejection of both rock and pyrrhotite in a single stage is discussed. A two-stage circuit simulation based on batch laboratory flotation data is also presented. Further development work, including the on-site pilot plant test work and the plant implementation of the new circuit design, will be described in a later publication.INTRODUCTIONThe milling operation at Inco’s Clarabelle mill, Copper Cliff, Ontario processes a rich complex sulfide ore from several large production mines around the Sudbury basin (Agar, 199 1). The sulfide mineralization is simple, consisting of chalcopyrite (Cp), pentlandite (Pn) and pyrrhotite (Po). The main gangue minerals are pyroxene, feldspar and quartz with minor amphibole and talc. Estimates of mineral composition are routinely calculated from Cu, Ni and S assays. In the mineralogical calculations, all gangue minerals are conveniently grouped and treated as rock (Rk)."

Jan 1, 2000

By P. L. Dunn

Automatic control of fine coal cleaning circuits has traditionally been limited by a lack of sensors. Although several nuclear-based analyzers are available, none have seen widespread acceptance. In this investigation, image analysis is used for on-line determination of ash content in fine coal slurries. The ash content of a flotation tailings stream is correlated to the mean gray level of the slurry. Based on a 90% prediction interval, an analysis of sensor performance indicates an accuracy of 73 ± 4% ash over a range from 65-90% ash. While this result is comparable to existing nuclear-based analyzers, the video-based system is only a fraction of the cost. The sensor is currently installed at the Middle Fork preparation plant owned by Pittston Coal Company.

Jan 1, 1997

By M. P. Saunders

A comprehensive, site specific water management plan is of primary importance to the successful implementation, operation and decommissioning of any mining project. Formulation of a plan requires an orderly planning process consisting of identifying problems, establishing planning criteria based on the guidelines and requirements of regulatory agencies, and an iterative procedure of identifying, investigating, and evaluating plan elements and alternative plans. A typical uranium surface mine operation consists of a mine pit or pits, spoil pile areas, crusher, processing plant, and tailings disposal area. A water control plan addresses the problems of diversion of natural runoff from undisturbed areas around the mine; and diversion, collection, and treatment of runoff from within the mine area. A water control plan is required to determine the location and size of appropriate control and detention structures; their cost; and the schedule for permit activity, design, and construction. The plan must be formulated to meet the requirements of both the State and Federal regulatory agencies.

Jan 1, 1981

By Bezalel C. Haimson

We have developed a new wireline hydrofracturing technique for in situ stress measurement which eliminates the need for drill rig and drill rod and renders the tripping of the straddle or impression packers continuous, fast, and inexpensive. Wireline conductors facilitate the use of downhole pressure transducers. We have confirmed the suitability of the new technique by testing it in a deep hole near the Monticello Reservoir, South Carolina. Our measurements indicate two local stress fields, one of them apparently linked to the impoundment-related induced seismicity.

Jan 1, 1984

By M. Eraydin

Dewatering fine particles (e.g., flotation concentrates) is inefficient, energy intensive and often requires large capital and operating costs. To address these issues, a series of advanced dewatering technologies have been developed at Virginia Tech. These include novel dewatering aids and a hyperbaric centrifuge. Typically, the moistures obtained using these methods are 30% to 50% lower than those obtained using conventional methods, as demonstrated in laboratory and pilot-scale test programs. With another novel dewatering technology that has been tested in bench-scale tests, the moistures can be reduced to levels once believed to be attainable only through the use of thermal dryers. In this communication, test results obtained with these advanced dewatering technologies are presented and compared.

Jan 1, 2014

By Rong Fan, Andrea Gerson

"The small recovery of Ag, often in the order of 50%, by traditional flotation and leaching process strategies is closely related to the complicated and heterogeneous nature of Ag-bearing minerals with Ag typically being associated with numerous minerals within any given ore-body. Moreover, grain sizes may be extremely fine with micron-scale common. In order to design cost-effective Ag-bearing mineral flotation and leach recovery strategies it is firstly necessary to characterise the speciation of Ag and its correlation with other elements, e.g., Cu, As and Sb, within the ore matrix and process streams. The traditional QEM-SCAN analytical approach can prove problematic for some mineralogies due to difficulties in recognising degrees of crystallinity, polymorphs, complex solid solution series, high value trace element mineralogy, very fine grained materials; and the requirement for a comprehensive data base. Moreover, there is difficulty ‘seeing’ either surface films or surface contaminants which can both lead to poor flotation performance. There is therefore clearly a need to develop confirmatory and complementary approaches when these uncertainties arise. Synchrotron X-ray microprobe analysis provides a powerful tool for the mineralogical characterisation of finely segregated heterogeneous Ag-containing materials. These measurements enable comprehensive characterisation of the Ag-bearing components using synchrotron microprobe X-ray fluorescence mapping (µ-XFM), X-ray diffraction (µ-XRD) and X-ray absorption spectroscopy (µ-XAS). Further analytical strength is provided by principal component analysis of laboratory ToF-SIMS data which enables the statistical identification of correlated surface species enabling examination of surface chemical characteristics responsible for mis-reporting of Ag containing components within flotation streams. We describe the application of these techniques to a selected Ag-containing feed and tail and demonstrate how these compliment and extend traditional analytical methodologies."

Jan 1, 2014

By Tony Moore, Peter Riccio

"MMG (Minerals and Metals Group) operates the LXML Sepon’s mineral processing facility located 40 kilometers north of the town of Sepon, in the Savannakhet Province, Lao PDR. The Sepon copper plant produces 70,000 tons/year of LME grade A copper cathode using leachautoclave- SX/EW-flotation technology (Baxter et al., 2003). Pyrite recovery in the flotation circuit is critical to the operation, as it is used to generate acid and soluble ferric ion for the leach circuit. Low pyrite recovery has been an on-going challenge in the flotation circuit. Final tailings characterization work done by Ian Wark Research Institute (Bassell et al., 2006) showed that the pyrite losses to the tailings are made up of a combination of coarse liberated particles and finer particles that have been coated by clay fines.In December of 2005, a joint Cytec-MMG technical program was initiated to address the pyrite recovery issue. A series of diagnostic tests were performed in the Cytec Stamford Research Facility using synthetic plant water showing that the collector was precipitating as a metal salt and was unavailable for mineral attachment. The tests also identified several collector chemistries that would likely be robust in the low pH (< 1), high dissolved metal ion environment and will not complex with metal ions in solution. With the support of the Sepon on-site engineers, an extensive, successful laboratory program was completed, which was subsequently followed by on-plant testing that showed a step wise improvement in pyrite recovery.INTRODUCTIONLow pyrite recovery has been an ongoing problem in the flotation circuit at Sepon. Sulfur recovery (indirect measure of pyrite) was improved from 40% to 65%-75% in mid-2005 when on site laboratory work confirmed that AERO® 8045 Promoter was a more effective collector then AERO® 407 Promoter. However, sulfur recoveries above 65% could never be achieved. Sepon requested the Ian Wark Research Institute to complete a surface analysis study using Scanning Electron Microscopy, X-ray Photoelectron Spectroscopy, and Time-of-Flight Secondary Ion Mass Spectroscopy to determine if clay coated pyrite played a role. It was concluded that the pyrite losses to the tailings are made up of a combination of coarse (+ 100µm) liberated particles and finer (-74µm) particles that have been coated by clay fines. The majority of the losses appear to be due to the former. In December of 2005, a series of diagnostic tests were performed in Stamford using synthetic plant water in order to identify what chemistries would likely be robust in the slimy low pH and high dissolved metal ion solution environment. Results showed that several chemistries would not complex with ferric, ferrous or copper in solution and therefore should perform well in the Sepon flotation. The incumbent collector, AERO® 8045 Promoter was complexing with metal ions in solution. This resulted in the collector not being available for adsorption onto pyrite."

Jan 1, 2011

By Peter Riccio

Oxiana Sepon?s mineral processing facility in south central Laos produces copper cathode using leach-autoclave-SX/EW flotation technology. Pyrite recovery in the flotation circuit is critical to the operation, as it is used to generate acid for the leach circuit. However, low pyrite recovery has been an ongoing challenge in the flotation circuit. Tailings characterization work done by Ian Wark Research Institute showed that the pyrite losses to the tail are made up of a combination of coarse liberated particles and finer particles that have been coated by clay fines. In December of 2005, a joint Cytec-Oxiana technical program was initiated to address the pyrite recovery issue. A series of diagnostic tests were performed in the Cytec Stamford Research Facility using synthetic plant water showing that the collector was precipitating as a metal salt, and was un-available for mineral attachment. The tests also identified several collector chemistries that would likely be robust in the Sepon low pH, high dissolved metal ion environment, and will not complex with ferric, ferrous, or copper in solution. With the support of the Sepon on-site engineers, an extensive, successful laboratory program was completed, which then was followed by on plant testing that showed a step wise improvement in pyrite recovery.

Jan 1, 2014

By R. Clark Lantz, Charles Stanley, David E. Hinton

Morphometric examination of lung alterations following exposure to toxic and pathogenic substances is invaluable in detecting subtle changes which might otherwise be overlooked. Results, from evaluation of initial structural alterations, support and guide subsequent nonmorphological techniques and, when coupled appropriately, can lead to a better understanding of the pathogenesis of a disease. Morphometry is a technique which can be used to determine, quantitatively, the site, extent, development, and progression of a disease state. Determination of the extent of tissue and cellular involvement in the response to inhalation of silica has been performed in order to gain a better understanding of the development of fibrosis following inhalation of this toxicant. In addition, since silica has been postulated as one of the important pathogenic minerals in coal mine dust' it is important to understand the lung tissue response to silica prior to evaluating the effects of inhaled coal mine dusts. Male Syrian golden hamsters were placed in Hazelton 1000 inhalation chambers and were exposed to either filtered air (controls, n = 24) or filtered air plus 30 mg!m3 silica (Min-U-Si110,95%ofparticles < 5 um,n =24)for16hours per day, five days per week, for two weeks. Animals were returned to the animal quarters prior to subsequent tissue processing at 1, 4, and 12 weeks following exposure. This schedule was designed to allow time for the development of fibrosis. Lungs were fixed by intratracheal perfusion of ½ strength Karnovsky's fixative at a pressure of 20 cm H20 and tissues were taken for embedment and analysis at the light level. Determination of site, extent, and development of alterations was performed using a multitiered approach. Changes in large structures (airways, blood vessels) was determined prior to evaluation of changes at the septal tissue level. Data will be presented outlining the progression of changes. Results indicate the usefulness of morphometric analysis of lungs following inhalation of toxic substances in quantitatively defining the structural alterations.

Mar 1, 1989

By Italo Onederra, Miguel Araos

"Most of the current explosives for mining blasting activities are based on ammonium nitrate (ANFO, emulsions and watergels). AN-based explosives’ detonating properties were discovered in the 50’s and since then they have proven to be reliable, safe and cost effective. However, there is no current alternative raw material to replace AN. As part of a program of research initially funded by the Australian Coal Association Research Program (ACARP), Mining3 and the University of Queensland engaged in the search for potential oxidizing agent alternatives that could replace AN in the manufacture of mining explosives. Candidates included those that are produced in large quantities and that can be cost competitive with ammonium nitrate. The initial work identified hydrogen peroxide (HP), calcium ammonium nitrate (CAN) and sodium nitrate (SN). Explosives mixtures were prepared and sensitized using void sensitization techniques. The detonation properties results showed that explosives based onmixtures of HP/CN and HP/SN as the oxidizer were able to detonate in a range of 2000 – 5000 m/s, indiameters 50 and 102 mm, and densities in the range of 0.50 – 1.40 g/ml. Although more studies need to be conducted to fully understand these explosives, if successful, these explosives would be able to offer an alternative to the AN-based explosives in the mining industry."

Jan 1, 2017

By V Pere

A geotechnical data management system has been developed to manage drillhole and mapping data generated from in house and consultant administered field programs. This system comprises a data collection interface with integrated validation, processing and reporting functionality. Development of the database required careful consideration of how and what geotechnical information is gathered in the field, a clear understanding of the interdependency of the parameters, and the outputs required to support geotechnical design.The acQuire Geoscientific Information Management System was selected as the preferred software to support the process because of its ability to easily manage data from capture in the field through to quality reviews and ultimately into reports. Initially only geotechnical logging of diamond core was considered, subsequently the system has been expanded to support additional geotechnical data sources such as laboratory test results, traverse and window mapping data. This paper focuses on the core logging data acquisition, validation, analysis and presentation of data.The acQuire Geoscientific Information Management System has proven to be a robust platform for the capture, interrogation, and reporting of geotechnical data. Data entry objects have been created to manage seven different areas of geotechnical data collection ranging from core logging to mapping to automated reports for falls of ground.

Aug 22, 2011

By S. Klemmer

Potential problems associated with groundwater inflows are a major concern to many Saskatchewan mining operations. Rock mass behaviour and stability have been linked to groundwater inflow potential. An estimate of the hydraulic conductivity of the rock mass is an important factor for assessing groundwater inflow potential. This paper presents a procedure that was developed to conduct air permeability tests, for estimating the hydraulic conductivity of the rock mass. Tests were conducted near underground excavations with the goal of assessing links between measured air permeability, rock mass classification and the rock mass response to adjacent excavations. Measured changes in air permeability corresponding to the distance from excavation boundaries may indicate changes to joint properties such as joint aperture, continuity and frequency. This study outlines the instrumentation, the experimental and analytical procedures, and the preliminary results from testing.

Nov 1, 2011

By Fawcett M

Pine Creek Goldfields Limited (PCG) is rehabilitating the Enterprise Pit to an aquatic habitat and potential water resource. This paper discusses the development and implementation of that plan. Pine Creek Goldfields Ltd&apos;s Enterprise Mine is located approximately 230 km south of Darwin. Open cut mining of the orebody commenced in 1985 and continued for eight years with 14 million m3 of ore and overburden extracted. Geochemical testing of exposed rock types in the pit has demonstrated a propensity to produce acid, and dissolved metals. A plan of management has been developed and implemented to minimise the risk of acid formation and improve the overall water quality in the final void. In developing the plan prime consideration was given to reducing the potential for pit water to impact upon the regional groundwater. An interception system was constructed on Pine Creek to divert approximately 9 km2 of upper catchment into the northern end of the Pit. Construction of the diversion system was completed in December 1993 and a comprehensive surface and groundwater monitoring program initiated. The monitoring program and associated studies are also discussed.

Jan 1, 1994

By Peter L. Swanson

NIOSH has developed an automated PC-based seismic monitoring system for use in mine ground control studies. The distribution and coordination of data acquisition and processing functions across a local area network (LAN) is a central feature of the system. Design requirements include (1) the use of lowcost, off-the-shelf, data acquisition and computer hardware, (2) the capability of automatically merging waveform data sets from different types of monitoring systems, (3) delivery of raw and processed data underground and on the surface, as well as to remote sites, and (4) automated data collection and processing over long periods of time without the need for human intervention. Current mine-wide installations utilize multiple, autonomous seismic networks located both underground and on the surface and provide 20 to 40 stations within a radius of several kilometers. Event locations, magnitude estimates, and other processed data are distributed for display and analysis on network nodes both underground and in the offices of mine engineers and managers. This paper describes the system&apos;s hardware and software components, examples of installations in hard-rock and coal mines, and a few observations from western U.S. longwall coal mines.

By R. V. Kumar, C. Schwandt, S. Subramanian, S. Sampath, S. Vimalnath

Waste waters and industrial effluents containing toxic heavy metals such as Pb, Hg, As and Cr, beyond permissible limits (0.1 ppm, 0.01 ppm, 0.02 ppm and 0.05 ppm, respectively) (WHO, 2011), pose serious threats to the ecosystem, when discharged into the environment. It thus becomes imperative to monitor the concentration of the toxic heavy metals and treat them before discharging. In this study with the above objective in mind, a microbial sensor has been developed using P. aeruginosa cells drop coated onto a carbon paste electrode (CPE) for the detection of Pb(II) ions in aqueous solutions. Detailed biosorption experiments confirmed that complete bioremoval of lead could be achieved using P. aeruginosa cells under optimum conditions of pH and biomass loading. For the electro-analytical determination, the Pb(II) ions were initially preconcentrated on the P. aeruginosa modified CPE, reduced and then anodically detected with oxidation of Pb0 using differential pulse anodic stripping voltammetry (DPASV) technique. All the experiments were carried out in the presence of 0.1 M sodium acetate buffer and KNO3 as background electrolyte. The parameters namely pH, scan rate, biomass loading, deposition potential, deposition time and concentration of Pb(II) ions were optimized for the biomass modified CPE, to obtain maximum voltammetric response. Under the optimized conditions of pH 5.6, scan rate of 0.008 V/s, biomass content of 1.7–1.8×109 cells/mL, deposition potential of –1.2 V, and deposition time of 500 s, the developed microbial sensor showed a linear response for the peak current between 0.5×10-5 M and 1×10-8 M of Pb(II) ions with the R2 value of 0.9979. The lowest limit of detection (LLOD) was determined as 2.4×10-7M of Pb(II) ions (0.05 ppm). The redox mechanisms of Pb(II) ions on the P. aeruginosa modified CPE surface are discussed.

Jan 1, 2016