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By Kafui Nyavor

The indirect, and the direct contact mechanisms of bacteria oxidation of sulfides were studied using Thiobacillus ferrooxidans and pyrite. The aim was to determine which of the two generally aeeepted mechanisms played a dominant role, if any, during acid mine drainage (AMD) formation from the oxidation of sulfide minerals. The results show that at a pH of 4.5 where T. ferrooxidans are quite active, but where initial chemical oxidation of pyrite to Fe2+ is limited, the bacteria were unable to directly oxidize pyrite to any significant extent. Whereas, in the presence of Fe2+, T. ferrooxidallS assisted oxidation of pyrite by the indirect mechanism was observed and sustained over the pH and Eb range where the bacteria is active. The results lead to the conclusion that the direct contactmechanism of pyrite oxidition by T. ferrooxidans is insignificant. The predominant, and possibly the only, pathway involves the indirect bacteria oxidation of available Fe2+ to Fe3+, which in turn oxidizes FeS2 to generate additional Fe2+ and acidity in a cyclic process.

Jan 1, 1996

By Rajan S, Mainwaring P, Gould WD, Beaulne M

Polished discs of a complex sulphide ore were leached with three lixiviants; a mixed culture of Thiobacillus ferrooxidans and Thiobacillus thiooxidans, Thiobacillus ferrooxidanalone, and ferric sulphate. At either one or two week intervals the discs were examined by scanning electron microscopy, and the amount of soluble metals released from the discs were determined. The mixed culture solubilized more Cu, Zn and Pb than did either ferric sulphate or T. ferrooxidans alone. However, no differ-ences were observed between the three treat-ments by scanning electron microscopy during the first 44 weeks. The minerals were attacked in the following order: pyrrhotite, cubanite, sphalerite, pyrite and chalcopyrite.

Jan 1, 1990

This brief introduction tries to introduce delegates to this conference to the almost unbelievable attributes possessed by many microbes which might be relevant to geomicrobiology, and how most of these remain unexploited by us. It introduces some of the terminology which will be used and tries to suggest that there are rewarding times for those who set out to discover the natural biodiversity of bacteria in nature. Although we have exploited microbes, usually unwittingly, and suffered at their hands for millennia, it is only recently that we have begun to become aware of how they dominate our planet (most of the earthÆs biomass is bacterial) and are responsible consequently for most of what happens here. A few can cause us harm and occasionally kill us, but most bacteria are beneficial, impacting positively for example on how we grow our food and in cleaning up the pollution we create. They are simply indispensable to how we live. They have been responsible for many of the geochemical changes which have taken place on this planet and play major roles in the transformation of elements so essential to our existence (Newman and Banfield, 2002). No one should dismiss them as minor players in our game of life.

Jan 1, 2004

By A. C. Headon, K. W. Lam, D. P. Dautovich

"Microbiological activities affecting the performance of materials have been encountered in diverse situations within Ontario Hydro. Case histories of bacterial problems which are presented include ground rod corrosion at transformer stations, corrosion of a bare steel cooling water intake structure in Lake Huron and corrosion of steel components in a nuclear generation station vacuum building. Possible microbiological activities which may affect the corrosion behaviour of containers for the disposal of nuclear fuel waste in the future are discussed, as are bacterial actions suspected of causing an odour problem in residential water heaters.IntroductionBacteria are ubiquitous. Except by use of sterilization methods, bacteria -free environments are no t possible to find. Bacteria lie dormant in hostile environments, spread easily through air and water currents and reproduce rapidly under favourable conditions. Although different types of bacteria have different preferable conditions for growth, they are highly adaptive and in general, whenever there is a source of water and carbon (carbon dioxide or organic matter), there will be bacterial activities . These activities often induce or enhance costly metallic corrosion in industries and water odour problems in rural water supplies . Underground structures and systems in contact with natural water or water containing organic matter are most susceptible to bacterial attack.The types of metal-degrading bacteria , their characteristics and the conditions for growth have been reviewed extensively in the literature(1,2,3). There are several often encountered groups of bacteria which, through their metabolic processes, may lead to the degradation of metals. A large variety of bacteria produce corrosive in organic or organic acids as waste, such as the well known formation of sulphuric ac id by aerobic sulphur oxidizers . Anaerobic sulphate reducers produce hydrogen sulphide which is corrosive and has an obnoxious smell, slime-forming bacteria from slime which shelters sulphate reducers and iron bacteria oxidize ferrous to ferric ion which precipitates as hydrated oxides creating oxygen concentration cells . The following are bacterial problems encountered in Ontario Hydro. Although the number of recognized cases has been few, they show the diversity of location and environment where bacterial problems could arise. These include ground rod corrosion at transformer stations, corrosion of bare steel in a water intake structure, corrosion of steel in a nuclear generation station vacuum building and an odour problem in residential hot water tanks. In addition, possible bacterial- enhanced corrosion in containers for future disposal of nuclear fuel wastes are also discussed."

Jan 1, 1984

ABSTRACT- I Chemotrophic bacteria, capable of oxidising inorganic species such as Fe(II) and S(-]I) for their metabolic energy, have been known for many decades and interest in these bacterial species has increased since their ability oxidise auriferous arsenopyrite ores has been demonstrated. The rate at which bacterial cultures can oxidise a solid sulphide ore is of importance but a number of difficulties arise when attempting to evaluate this oxidative activity. The two major problems are counting the number of viable bacteria present in the reactor and identifying the types of bacteria that are active in both the solid and liquid phases. Enumerating the numbers of bacteria responsible for an observed change is necessary to compare the activity of different isolates. For selection and identification of bacteria during the development stages of a project, and for monitoring an operational process, the recognition of different species will be of importance. Methods for the taxonomic discrimination of bacterial species rely on observations, different culturing techniques, and the degree of DNA base homology between isolates. A method reported recently uses IR spectroscopy for comparisons between isolates. This spectroscopic method and its application is discussed.

Jan 1, 1991

By Hong Nguen Vu, Pavla Ovcarí, Peter Fecko, Barbara Tora

The aim of this paper is to evaluate the suitability of a coal sample from the mine Jaworzno (Poland)- locality for bacterial leaching. Laboratory research used a pure bacterial culture of Thiobacillus ferrooxidans. The results showed that bacterial leaching was effective, with a one month leaching time, when 65% of total sulphur and 85% of pyritic sulphur was leached. The paper describes changes to the pyrite during and after bacterial leaching.

Jan 1, 2003

By Hong Nguen Vu, Pavla Ovcarí, Peter Fecko, Barbara Tora

"The goal of the work was verification of bacterial leaching on the samples of black coal. From the results obtained it was clear that the coal is very well leached by Thiobacillus ferrooxidans and this technology can be used for coal desulphurization. A leaching time of one month is required, to remove 65% of total sulphur and 85% of pyritic sulphur.Microscopic evaluation of samples from Jaworzno showed, that it should be classed as a coal with low coalification, probably from black coal hemiphase. Macerals of the liptinite group were well distinguishable; they were of a dark brown colour. Evidence of coal mass oxidation was apparent on the fresh sample; in the test samples (during bacterial leaching) small grains were oxidised."

Jan 1, 2003

By R. W. Smith

A microorganism will function as a mineral flocculant if it is to some degree hydrophobic and can attach itself in some manner, such as through electrostatic interaction, to the fine mineral particles to be flocculated. The present paper concerns the use of a hydrophobic bacterium, Mycobacterium phlei, as flocculating agent for phosphate slimes. Experimental work on the flocculation of phosphate slimes and of dolomite and clay mineral suspensions was performed. It was found that M. phlei functions as an excellent flocculant for a dolomitic slime but does not function as well for mineral suspensions where large quantities of the solids are finer in size than the approximately 1 µm size of the bacterium.

Jan 1, 1992

By A. Gupta, S. C. Srivastava, K. K. Saroj

Bacterial degasification of methane and conventional methane drainage for generation of power have become accepted practice. Comparative economies of both the techniques have been described. Authors have discussed an alternative technique, involving complete methane biocycle. The first stage of the entire process involves gasification of coal using a consortia of bacteria to generate methane. The generated methane has been proposed to be pumped,as in conventional methane drainage. Technique described is basically similar to underground coal gasification, with the difference that in place of coal combustion, degradation of coal to lower molecular weight compounds by one set of bacteria and then methanogenesis by methanogens, producing methane have been described. Brief account of communities of bacteria that may be employed have been dealt with. Three arguments in favour of proposed technology as an alternative to conventional coal mining are: 1.Total elimination of hazardous operation. 2.Availability of power plants based on methane drainage, assuring comparative economies. 3.Possibility of utilisation of fuel cell technology for power generation giving higher efficiency. All the three phases of complete blocycle viz (1) methane generation from coal (ii) conversion of methane to hydrogen and oxygen (iii) conversion of products so obtained to methane, thereby completing the cycle have been discussed. INTRODUCTION Drainage of methane by conventional pumping process (Boxho,et.al.,1980) not only is helpful in maintaining safe extraction of coal, but with utilisation of extracted gas for power generation, also helps in over all economy of coal mining. This is gradually becoming standard practice for mining of coal in highly gassy mines. An alternative technique (Thakur et. al., 1991) of bacterial degasification of coal seams is also gradually gaining ground. The bye products of this process that is carbondioxide and water, do not contribute towards economy of coal extraction process. However the technique has its own advantage particularly in goaf area for avoiding fire hazard and drainage of methane from coal seams prior to initiation of mining operation. Either of these techniques could be applied in specific situations with success.

Jan 1, 1993

By Sergio Bustos

Covellite resulting from an original 0.5 % Cu(tot) chalcocite ore has been leached in columns at different temperatures, in the lower range of 10 - 20 °C, under acid - ferric bacterial leaching conditions. Dissolution of copper from covellite depends on the rate and extent of sulphide oxidation by ferric ion. While it is recognised that the dissolution of sulphide minerals is electrochemical in nature, an apparent chemically controlled dissolution pattern was measured with activation energy of 78.09 kJ/mol (18.66 kcal/mol). Ferric iron is the main oxidant and its production and availability will depend on the bacterial activity developed in the system. This activity is characterised by the population size and by the specific oxidation rate of the bacteria. Bacterial growth was followed directly by the number of bacteria appearing in the column effluent solutions and indirectly by the generation of ferric iron or by the total oxidation observed in the columns. These results have been interpreted by using the heap oxidative capacity parameter, which indicates an equivalent ferric production of about 0.07 - 0.15 kg Fe +3/t/day at 20°C. Although bacterial activity decreases to about 0.03 kg Fe +3/t/day with decreasing temperature, a significant bacterial oxidation is still observed at 10 °C. However, extended lag times are observed, which delay the start of bacterial growth to times concurring with covellite leaching. Under these conditions, bacterial activity is displayed through a population controlled by the rate at which- ferrous iron is released from the reducing covellite surface. These results suggest the potential benefit of including some kind of inoculation when operating at lower temperatures. Also, heat loss control and/or heat addition to the heaps should be considered during process design and operation of ferric bacterial heap leaching of secondary copper sulphide ores at temperatures lower than 20°C.

Jan 1, 1999

By J. R. Jutras, C. Brissette, J. Champagne

"Pure cadmium sulphide was leached by the action of the bacterium Thiobacillus thiooxidans in the presence of finely divided sulphur. The influence of time, temperature, pH and concentration of inoculum on the extent of the cadmium extraction was studied. Under optimum conditions, up to 72 per cent of the cadmium could be dissolved over a six-week period. A cursory study of the role played by elemental sulphur seems to indicate that its presence is essential but that the sul1>huric acid produced through its oxidation is a minor factor in the cadmium extraction."

Jan 1, 1971

By Madgwick J. C

Bacterial leaching of museum grade chalcopyrite and chalcopyrite concentrates at low sorptive relative humidities (55-91 per cent rH) was demonstrated. Optimum release rates were 13 and 27 mg/gram/100 days copper (Cu++) at 91 per cent and 80 per cent rH and 12 and 33 mg/gram/100 days iron (Fe++) at 80 per cent and 73 per cent rH for concentrate and "pure" chalcopyrite respectively. The optimum copper release rates occurred between 73 per cent - 86 per cent rH and 30-40 mM acid concentrations. Bacterial copper leaching rates were some twenty times slower for emersed copper concentrates, at low relative humidity, compared with submerged cultures. Bacterial copper solubilization was almost quantitatively inhibited above 150 mM acid levels (< pHO.8).

Jan 1, 1984

Bacterial Leaching of Complex Polymetallic Sulfide Ore The work described here concerned the study on bacterial leaching of complex sulfide minerals and polymetallic ores operated in shaken flasks and small-scale columns. The bioleaching of jamesonite was not accessible. The iron extraction in pyrrhotite leaching reached 98.2 per cent after 23 days. After 26 days, the zinc extraction of marmatite reached 92.3 per cent while the iron extraction only reached 13.6 per cent. The pulp density had effect on the metal extraction of pyrrhppite and marmatite. For complex polymetallic sulfide ores, after 10 days in shaken flasks, 97.1 per cent zinc extraction was achieved. While after 60 days in small-scale column, the zinc extraction only proceeded to 7.8 per cent; By SEM and EDXA of experimental samples and their leaching residuum, the bioleaching mechanism was discussed.

Sep 13, 2010

By J. R. Fisher

"Uranium may be leached to some extent in situ from the Blind River conglomerate uranium ores by using the products of bacterial activity from the bacterium Thio-bacillus ferrooxidans his paper describes some of the laboratory techniques used to investigate the characteristics of the bacteria, and to utilize their properties in uranium leaching. The application of some of the laboratory results underground at the Milliken mine of Rio Algom Mines Limited are de-scribed, along with the method of uranium recovery from the mine waters."

Jan 1, 1966

By Zhang Yongzhu

Developments on bioleaching of low grade ores and refractory gold ores by T. Ferrooxidans in China are summarized. Effect of the ions on bacterial growth, bioelectrochemical reaction mechanism of sulfide minerals and bacterial reaction mechanism are also discussed.

Jan 1, 1992

By R. A. MacGregor

The Stanreck mine of Stanreck Uranium Mines Limited in located on Quirks Lake in the Elliot lake - Blind River area of Northern Ontario. It is approximately 25 miles north of the north shore of Lake Huron, equidistant between the cities of Sudbury and Sault Ste. Marie. The property was developed ad brought into production at a rate of 3,000 tens of ore/day during the uranium boom of the mid 1950's. By 1960 mine waters were becoming quite acid through the activity of bacteria. Treatment of this water was begun for pollution control and a small amount of uranium which had been leached from the ore by these acid waters was recovered. Development of bacterial leaching with a view to increasing production by this method commenced in December 1962. Subsequently, it was succeesfull in producing 10,000 - 15,000 lbs. of U308/month. Since late 1964 uranium production has been obtained solely from bacterial leaching. A rare earth concentrate was also made for its yttrium content during 1966 and 1967. This paper describes the development, operation and methods used in the underground bacterial leaching process.

Jan 1, 1969

By B. J. Ralph, I. J. Corrans, B. Harris

In the first section, a brief background to the practical and theoretical aspects of bacterial leaching is presented. Descriptions are given of present and potential methods for exploiting bacterial leaching in metal-winning. In the second section, the results of recent research are presented. The aim of this work was to obtain a greater understanding of the mechanism of bacterial leaching of sulphide minerals. Bacteria were observed to catalyse leaching reactions by oxidising ferrous ions to ferric ions, by removing protective films at the mineral surface and by depolarising cathodic areas.

By Z. Manafi

Bioleaching of a copper sulfide ore containing "activated pyrite", using a mixed culture of mesophilic bacteria, was studied. This type of copper ore had been found unsuitable for flotation because it produces a concentrate containing relatively high iron concentrations and, therefore, a low copper grade. Microscopic observations showed that pyrite particles had been activated by the formation of a thin film of chalcocite. Hence, a bioleaching process was considered as an alternative to treat this ore. Following shake flask and acid consumption tests, column tests of the crushed ore (75% -12.5 mm) were initiated and the effects of culture medium, agglomeration, and irrigation type on copper recovery were studied. The results showed that all the columns containing bacteria had copper recoveries around 90% compared to the abiotic control column with a recovery of 50%. It was concluded that bioleaching could be successfully utilized to process copper ores containing activated pyrite.

Jan 1, 2007

By Wolfgang Sand, Alex Schippers, Tilman Gehrke, Thore Rohwerder, Peter-Georg Jozsa

Metal sulfides are degraded via thiosulfate (pyrite) or via polysulfides and sulfur (sphalerite, galena, chalcopyrite) by leaching bacteria The attack on the crystal lattice is either effected by only iron(III) ions or by iron(III) ions and protons, respectively. Consequently, different endproducts are formed. For a control of ARD-processes, these reactions need to be hown to regulate them. Experiments in percolators up to 65 m3 with inorganic compounds like fluoride, concrete, or SDS resulted in differently lasting inhibition. Microcalorimetric measurements of microbial leach activity allow monitoring of these effects with respect to active bacterial cells, and, in addition, the amount or rate of dissolved metal sulfide allowing prediction of possible endangerment by ARD.

Jan 1, 2000

By Budden J. R, Rhodes M. K

Bacterial oxidation technology was successfully applied in a mine site pilot plant for the treatment of refractory gold concentrates. The bacterial culture was proven capable of selectively breaking down the refractory sulphide minerals to release the gold. Gold extractions greater than 90 per cent were possible when oxidised concentrate was leached with cyanide solution. The pilot plant was designed using data scaled up from laboratory testing and sized for transport by standard vehicles. The bacterial culture used to break down the refractory gold-bearing mineral was a natural mixed culture able to withstand temperatures up to 55¦C, thus making the technology particularly suitable for use in Australian and in some North American gold mining regions. The pilot plant was operated at the mine site over a six month period. Performance of the bacterial oxidation technology under site conditions was as predicted from the laboratory testing. It was demonstrated that the culture was robust under normal site condi- tions and that the culture could withstand wide variations in the feed and the operating conditions, while still achieving satisfactory oxidation levels. The technology was able to be utilised by site operators and supervisors with minimum intervention. Design data for a commercial facility was collected for a number of different concentrate samples and a feasibility study completed. Estimated capital and operating cost have been obtained.

Jan 1, 1991