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By A. C. Headon, K. W. Lam, D. P. Dautovich

"Microbiological activities affecting the performance of materials have been encountered in diverse situations within Ontario Hydro. Case histories of bacterial problems which are presented include ground rod corrosion at transformer stations, corrosion of a bare steel cooling water intake structure in Lake Huron and corrosion of steel components in a nuclear generation station vacuum building. Possible microbiological activities which may affect the corrosion behaviour of containers for the disposal of nuclear fuel waste in the future are discussed, as are bacterial actions suspected of causing an odour problem in residential water heaters.IntroductionBacteria are ubiquitous. Except by use of sterilization methods, bacteria -free environments are no t possible to find. Bacteria lie dormant in hostile environments, spread easily through air and water currents and reproduce rapidly under favourable conditions. Although different types of bacteria have different preferable conditions for growth, they are highly adaptive and in general, whenever there is a source of water and carbon (carbon dioxide or organic matter), there will be bacterial activities . These activities often induce or enhance costly metallic corrosion in industries and water odour problems in rural water supplies . Underground structures and systems in contact with natural water or water containing organic matter are most susceptible to bacterial attack.The types of metal-degrading bacteria , their characteristics and the conditions for growth have been reviewed extensively in the literature(1,2,3). There are several often encountered groups of bacteria which, through their metabolic processes, may lead to the degradation of metals. A large variety of bacteria produce corrosive in organic or organic acids as waste, such as the well known formation of sulphuric ac id by aerobic sulphur oxidizers . Anaerobic sulphate reducers produce hydrogen sulphide which is corrosive and has an obnoxious smell, slime-forming bacteria from slime which shelters sulphate reducers and iron bacteria oxidize ferrous to ferric ion which precipitates as hydrated oxides creating oxygen concentration cells . The following are bacterial problems encountered in Ontario Hydro. Although the number of recognized cases has been few, they show the diversity of location and environment where bacterial problems could arise. These include ground rod corrosion at transformer stations, corrosion of bare steel in a water intake structure, corrosion of steel in a nuclear generation station vacuum building and an odour problem in residential hot water tanks. In addition, possible bacterial- enhanced corrosion in containers for future disposal of nuclear fuel wastes are also discussed."

Jan 1, 1984

ABSTRACT- I Chemotrophic bacteria, capable of oxidising inorganic species such as Fe(II) and S(-]I) for their metabolic energy, have been known for many decades and interest in these bacterial species has increased since their ability oxidise auriferous arsenopyrite ores has been demonstrated. The rate at which bacterial cultures can oxidise a solid sulphide ore is of importance but a number of difficulties arise when attempting to evaluate this oxidative activity. The two major problems are counting the number of viable bacteria present in the reactor and identifying the types of bacteria that are active in both the solid and liquid phases. Enumerating the numbers of bacteria responsible for an observed change is necessary to compare the activity of different isolates. For selection and identification of bacteria during the development stages of a project, and for monitoring an operational process, the recognition of different species will be of importance. Methods for the taxonomic discrimination of bacterial species rely on observations, different culturing techniques, and the degree of DNA base homology between isolates. A method reported recently uses IR spectroscopy for comparisons between isolates. This spectroscopic method and its application is discussed.

Jan 1, 1991

By Hong Nguen Vu, Pavla Ovcarí, Peter Fecko, Barbara Tora

The aim of this paper is to evaluate the suitability of a coal sample from the mine Jaworzno (Poland)- locality for bacterial leaching. Laboratory research used a pure bacterial culture of Thiobacillus ferrooxidans. The results showed that bacterial leaching was effective, with a one month leaching time, when 65% of total sulphur and 85% of pyritic sulphur was leached. The paper describes changes to the pyrite during and after bacterial leaching.

Jan 1, 2003

By Hong Nguen Vu, Pavla Ovcarí, Peter Fecko, Barbara Tora

"The goal of the work was verification of bacterial leaching on the samples of black coal. From the results obtained it was clear that the coal is very well leached by Thiobacillus ferrooxidans and this technology can be used for coal desulphurization. A leaching time of one month is required, to remove 65% of total sulphur and 85% of pyritic sulphur.Microscopic evaluation of samples from Jaworzno showed, that it should be classed as a coal with low coalification, probably from black coal hemiphase. Macerals of the liptinite group were well distinguishable; they were of a dark brown colour. Evidence of coal mass oxidation was apparent on the fresh sample; in the test samples (during bacterial leaching) small grains were oxidised."

Jan 1, 2003

By R. W. Smith

A microorganism will function as a mineral flocculant if it is to some degree hydrophobic and can attach itself in some manner, such as through electrostatic interaction, to the fine mineral particles to be flocculated. The present paper concerns the use of a hydrophobic bacterium, Mycobacterium phlei, as flocculating agent for phosphate slimes. Experimental work on the flocculation of phosphate slimes and of dolomite and clay mineral suspensions was performed. It was found that M. phlei functions as an excellent flocculant for a dolomitic slime but does not function as well for mineral suspensions where large quantities of the solids are finer in size than the approximately 1 µm size of the bacterium.

Jan 1, 1992

By A. Gupta, S. C. Srivastava, K. K. Saroj

Bacterial degasification of methane and conventional methane drainage for generation of power have become accepted practice. Comparative economies of both the techniques have been described. Authors have discussed an alternative technique, involving complete methane biocycle. The first stage of the entire process involves gasification of coal using a consortia of bacteria to generate methane. The generated methane has been proposed to be pumped,as in conventional methane drainage. Technique described is basically similar to underground coal gasification, with the difference that in place of coal combustion, degradation of coal to lower molecular weight compounds by one set of bacteria and then methanogenesis by methanogens, producing methane have been described. Brief account of communities of bacteria that may be employed have been dealt with. Three arguments in favour of proposed technology as an alternative to conventional coal mining are: 1.Total elimination of hazardous operation. 2.Availability of power plants based on methane drainage, assuring comparative economies. 3.Possibility of utilisation of fuel cell technology for power generation giving higher efficiency. All the three phases of complete blocycle viz (1) methane generation from coal (ii) conversion of methane to hydrogen and oxygen (iii) conversion of products so obtained to methane, thereby completing the cycle have been discussed. INTRODUCTION Drainage of methane by conventional pumping process (Boxho,et.al.,1980) not only is helpful in maintaining safe extraction of coal, but with utilisation of extracted gas for power generation, also helps in over all economy of coal mining. This is gradually becoming standard practice for mining of coal in highly gassy mines. An alternative technique (Thakur et. al., 1991) of bacterial degasification of coal seams is also gradually gaining ground. The bye products of this process that is carbondioxide and water, do not contribute towards economy of coal extraction process. However the technique has its own advantage particularly in goaf area for avoiding fire hazard and drainage of methane from coal seams prior to initiation of mining operation. Either of these techniques could be applied in specific situations with success.

Jan 1, 1993

By J. R. Jutras, C. Brissette, J. Champagne

"Pure cadmium sulphide was leached by the action of the bacterium Thiobacillus thiooxidans in the presence of finely divided sulphur. The influence of time, temperature, pH and concentration of inoculum on the extent of the cadmium extraction was studied. Under optimum conditions, up to 72 per cent of the cadmium could be dissolved over a six-week period. A cursory study of the role played by elemental sulphur seems to indicate that its presence is essential but that the sul1>huric acid produced through its oxidation is a minor factor in the cadmium extraction."

Jan 1, 1971

Bacterial Leaching of Complex Polymetallic Sulfide Ore The work described here concerned the study on bacterial leaching of complex sulfide minerals and polymetallic ores operated in shaken flasks and small-scale columns. The bioleaching of jamesonite was not accessible. The iron extraction in pyrrhotite leaching reached 98.2 per cent after 23 days. After 26 days, the zinc extraction of marmatite reached 92.3 per cent while the iron extraction only reached 13.6 per cent. The pulp density had effect on the metal extraction of pyrrhppite and marmatite. For complex polymetallic sulfide ores, after 10 days in shaken flasks, 97.1 per cent zinc extraction was achieved. While after 60 days in small-scale column, the zinc extraction only proceeded to 7.8 per cent; By SEM and EDXA of experimental samples and their leaching residuum, the bioleaching mechanism was discussed.

Sep 13, 2010

By J. R. Fisher

"Uranium may be leached to some extent in situ from the Blind River conglomerate uranium ores by using the products of bacterial activity from the bacterium Thio-bacillus ferrooxidans his paper describes some of the laboratory techniques used to investigate the characteristics of the bacteria, and to utilize their properties in uranium leaching. The application of some of the laboratory results underground at the Milliken mine of Rio Algom Mines Limited are de-scribed, along with the method of uranium recovery from the mine waters."

Jan 1, 1966

By Zhang Yongzhu

Developments on bioleaching of low grade ores and refractory gold ores by T. Ferrooxidans in China are summarized. Effect of the ions on bacterial growth, bioelectrochemical reaction mechanism of sulfide minerals and bacterial reaction mechanism are also discussed.

Jan 1, 1992

By R. A. MacGregor

The Stanreck mine of Stanreck Uranium Mines Limited in located on Quirks Lake in the Elliot lake - Blind River area of Northern Ontario. It is approximately 25 miles north of the north shore of Lake Huron, equidistant between the cities of Sudbury and Sault Ste. Marie. The property was developed ad brought into production at a rate of 3,000 tens of ore/day during the uranium boom of the mid 1950's. By 1960 mine waters were becoming quite acid through the activity of bacteria. Treatment of this water was begun for pollution control and a small amount of uranium which had been leached from the ore by these acid waters was recovered. Development of bacterial leaching with a view to increasing production by this method commenced in December 1962. Subsequently, it was succeesfull in producing 10,000 - 15,000 lbs. of U308/month. Since late 1964 uranium production has been obtained solely from bacterial leaching. A rare earth concentrate was also made for its yttrium content during 1966 and 1967. This paper describes the development, operation and methods used in the underground bacterial leaching process.

Jan 1, 1969

By Z. Manafi

Bioleaching of a copper sulfide ore containing "activated pyrite", using a mixed culture of mesophilic bacteria, was studied. This type of copper ore had been found unsuitable for flotation because it produces a concentrate containing relatively high iron concentrations and, therefore, a low copper grade. Microscopic observations showed that pyrite particles had been activated by the formation of a thin film of chalcocite. Hence, a bioleaching process was considered as an alternative to treat this ore. Following shake flask and acid consumption tests, column tests of the crushed ore (75% -12.5 mm) were initiated and the effects of culture medium, agglomeration, and irrigation type on copper recovery were studied. The results showed that all the columns containing bacteria had copper recoveries around 90% compared to the abiotic control column with a recovery of 50%. It was concluded that bioleaching could be successfully utilized to process copper ores containing activated pyrite.

Jan 1, 2007

By Budden J. R, Rhodes M. K

Bacterial oxidation technology was successfully applied in a mine site pilot plant for the treatment of refractory gold concentrates. The bacterial culture was proven capable of selectively breaking down the refractory sulphide minerals to release the gold. Gold extractions greater than 90 per cent were possible when oxidised concentrate was leached with cyanide solution. The pilot plant was designed using data scaled up from laboratory testing and sized for transport by standard vehicles. The bacterial culture used to break down the refractory gold-bearing mineral was a natural mixed culture able to withstand temperatures up to 55¦C, thus making the technology particularly suitable for use in Australian and in some North American gold mining regions. The pilot plant was operated at the mine site over a six month period. Performance of the bacterial oxidation technology under site conditions was as predicted from the laboratory testing. It was demonstrated that the culture was robust under normal site condi- tions and that the culture could withstand wide variations in the feed and the operating conditions, while still achieving satisfactory oxidation levels. The technology was able to be utilised by site operators and supervisors with minimum intervention. Design data for a commercial facility was collected for a number of different concentrate samples and a feasibility study completed. Estimated capital and operating cost have been obtained.

Jan 1, 1991

By A. E. McIlhinney, I. E. Puddington, C. E. Capes, A. F. Sirianni

"Two main problems are associated with the removal of pyritic sulphur from coals. The first is due to the fine state of dissemination of pyrite, which requires grindin1 to a very fine size to accomplish liberation. The second problem is caused by the similar surface chemical characteristics of coal and pyrite, which complicates separatio11 methods based on this property. In the work described here, the problem of fine particle size was overcome by using the selective oil agglomeratioa method developed at the National Research Council of Canada. Recovery of more than 90% of the combustible values was possible, even with particles only a few microns in diameter. Experiments using conventional chemical depressants for pyrite met with little success. It was found, however, that pyrite may be rejected during coal agglomeration through treatment with the iron.oxidizing bacteria, thiobacillus-ferrooxidans. The conditions for maximum pyrite separation are described and possible mechanisms by which the bacteria act are discussed."

Jan 1, 1973

By M. Stefanakis, A. Kontopoulpus, K. Adam

The application of Bacterial Oxidation as a pretreatment step for the extraction of gold from the Olympias refractory pyrite concentrate is described. A wide review of Bio-oxidation is presented focusing on the current process developments and commercial applications relevant to the treatment of gold bearing sulphide ores and concentrates. Preliminary batch and semi- continuous tests on the Olympas concentrate indicated that with Bacterial Oxidation quantitative gold liberation may be achieved. fie effect of such parameters as pH, Eh, temperature, particle size, operating pulp density and retention time on the extent of sulphur oxidation and subsequent gold extraction by conventional cyanidation are also examined. Finally, a preliminary evaluation of Bacterial Oxidation as a potential process alternative for the treatment of the Olympias concentrate is presented.

Jan 1, 1989

By Ü. Atalay, G. Alaeddinoglu, G. Özcengiz, G. Özbayoglu, Z. Özdemir

In this study, bacterial pre-treatment was applied before cyanidation in order to enhance the solubilization of silver from Artvin-Kafkasör ore Direct cyanidation process was found inefficient to recover silver from the ore. Only 40.36% silver recovery was obtained with direct cyanidation while silver recoveries of above 60% were obtained after bacterial treatment. Thiobacillus ferrooxidans was used as the microorganism in pretreatment tests. Effects of various parameters such as pulp density, duration, inoculum size and particle size on silver recovery have been investigated in this study.

Jan 1, 2001

By J. R. Parga

In mineral processing, poor froth-flotation performance translates into both an enormous loss of revenue and an unnecessary waste of the ore reserve. In this context, Bacís Mining Company developed a versatile new, high-performance flotation-cell design that can be incorporated into various flotation circuits. The main feature of this circular cell is that it is comprised of two separate sections, namely, a flotation cell that uses wash water to minimize the entrainment of fine gangue particles into the froth product and an external bubble generator that produces small bubbles of relatively uniform size at a specified aeration-rate. The air/water mixture is blended with the pulp by gentle agitation, so as to enhance particle-bubble aggregate-stability. Comparative metallurgical performance with conventional flotation cells show that the power requirements and operating costs for precious metal flotation are three times lower, while the concentrate grade is 7% higher and the recoveries of precious metals are 5% higher.

Jan 1, 2009

By Ralf Plinninger, Pafos Busch, Richard Gradnik

This paper presents the details of the Bad Bergzabern tunnel project. This tunnel is the main part of the B427 federal road bypass, intended to improve the regional traffic infrastructure and to reduce the traffic load in the historic city centre of the health resort Bad Bergzabern. Located in the south-western part of Germany, the project area is situated at the eastern margin of the so-called Palatinate Forest, a low mountain region of mainly triassic sediments with a complex and variable geological history. The German federal government, represented by the office of mobility of Rhineland- Palatinate, awarded the contract to BeMo Tunnelling, a specialized Tunnelling company with a long history of innovation and success in NATM tunnelling. The contract includes the construction of two separate tubes with a length of approximately 1,44 km (0.9 mi)—the road (main) tunnel with a cross section of 101 m2 (1,090 sq ft) and a parallel rescue tunnel with a cross section of ca. 23 m2 (250 sq ft.) Both are connected by six cross passages. Tunnels and cross passages are excavated according to the principles of the New Austrian Tunnelling Method (NATM). Due to BeMo Tunnelling’s technical expertise and capabilities, the project team was able to engineer a speed-up concept that saves money and time for the construction team and client. This paper presents the innovative approach BeMo Tunnelling made to optimize the execution of the tunnelling works and the challenges the project team is facing with regard to a complicated geological situation, countermeasures against an increasing effect of climate change and volatile prices for construction materials.

Jun 13, 2023

By S. K. Kawatra

It is common in coal-cleaning operations to produce a fine waste stream which contains a considerable amount of coal, but which is not economical to recover both because it is mixed with large amounts of difficult-to-remove clay slimes, and because the fines are often heavily oxidized. Conventional froth flotation machines cannot produce an acceptable product from such material in a single stage of flotation, and even flotation columns often have difficulty cleaning this type of feed. A new type of flotation column was designed specifically to deal with these problems, and was installed and tested in a coal-cleaning plant in Ohio. The feed coal contained 39.8% ash as fine clay, and 2.83% total sulfur, and was 80% passing 176 micrometers. The coal was a thickener product which had oxidized for some time, and was very difficult to float. However, using the new column it was possible to recover up to 85% of the calorific value in a product containing less than 11% ash, and up to 60% of the calorific value in a product of less that 8% ash. The bulk of the pyritic sulfur was rejected from the coal as well. Multistage flotation tests showed that the flotation column was able, in a single stage, to produce the best froth flotation product that it was possible to recover from this coal.

Jan 1, 1994

By Peter Amelunxen, Craig Lockhart

Significant effort was made in 2003 and 2004 to upgrade the existing instrumentation and process control infrastructure at the Phelps Dodge Bagdad copper concentrator. Specifically, • cameras were installed on the feed belt for online image analysis data, • ultrasonic level sensors were installed for froth height and sump level control, • a trial froth image system was installed on one of the rougher rows, and • a new expert system to tie everything together was integrated and tuned in-house. Control philosophy, observations, opinions, and general tips and tricks are discussed with the hope of assisting other plant personnel and expert system integrators.

Jan 1, 2005