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By K. L. Rubow

"IntroductionAir sampling for airborne contaminants requires a system for moving air, a collection method, and a procedure to determine the quantity of contaminant collected. Because occupational exposure limits and air quality standards frequently are expressed in terms of concentrations, a method of determining the volume of air sampled is also needed. The four principal components in a sampling train are shown in Figure 12-1. The inlet admits the air sample into the train; the collector(s) separates the gas, vapor, or particles from the air; the flowmeter measures the rate or total quantity of air sampled; and the pump (air mover) provides the suction required to draw an air sample through the train. Inlet sampling considerations are discussed in Chapter 21. Various types of collectors are described in subsequent chapters. Flowmeters are discussed in Chapter 7.This chapter will describe the air mover portion of the sampling train and air sampling systems that contain three or four components in a convenient package. The chapter consists of two parts. The first part contains a brief description of the different types of air movers and air sampling systems. The second part consists of tables and figures showing detailed information on a wide variety of commercially available air movers and sampling systems. ient size is filled with a liquid in which the suspected contaminant is insoluble. When the liquid is allowed to escape from the vessel, it is replaced by air from the atmosphere to be sampled. Such an air sample is an integrated sample rather than an instantaneous sample because the mass of fluid requires a finite time to empty. In fact, by employing a large vessel with controlled drainage, this method can be used to move air at low flow rates through a sample collector for extended intervals. Additional discussion of this method can be found in Chapter 17."

Dec 1, 1996

By R. V. Ramani

"Exposure to respirable contaminants in mine atmospheres has long posed a serious hazard to miners. The control of these hazards, some of which can have sudden and catastrophic effects and some others, slow and long enduring consequences, has been a major concern for labor, management and government alike. This concern has manifested itself in four primary mechanisms of control -- (1) regulatory control through minimum standard setting by the passage of mine health and safety laws, (2) engineering control through design and operation of 'mines according to the best recommended practices, (3) medical control through periodic physical examinations, wearing personal protection devices, etc., and (4) legal and social control through workmen's compensation laws for occupation related health deterioration:-The respirable contaminants in mine atmo¬spheres are toxic and explosive gases, toxic and explosive dusts, diesel exhaust particu¬lates, radon progeny, etc. Perhaps the most important of all these and the most widely occurring in mine atmospheres is respirable dust, an inevitable product of fragmentation, a unit operation fundamental to all mining operations. In fact; the-most serious health hazard for workers in the mining and minerals processing industries is still the exposure to respirable dust, and solutions to the problem of respirable dust disease remain to be found.The 1969 Coal Mine Health and Safety Act and its provisions for the respirable dust standards in underground coal mines are briefly reviewed. An overview of the research accomplishments in meeting the standards is presented. The current state of knowledge on the effectiveness of the standards and the new and emerging problems are discussed. A brief summary of the research programs of the Generic Technology Center on Respirable Dust is presented.In general, underground mines are becoming increasingly safe. The mine atmospheric environment is definitely improving * Professor and Head, Department of Mineral Engineering,-The Pennsylvania State University, University Park, PA for the better. Significant progress has been made in lowering the dust levels in mines. It is generally agreed that mines are less dusty today than they were a decade ago. However, as long as the risks of occupation-related diseases are not completely eliminated, vigilance can hardly be relaxed, and the search for engineering solutions must be intensified."

Jan 1, 1988

By H. S. Chiang, S. S. Peng

"SummaryAir velocity distribution surveys on four mechanized longwall faces are presented and discussed. Although there are discernible patterns in the velocity distribution, there are wide differences in distribution in the cases examined. This can be attributed to differences in mining and face geometry. An important factor is air leakage into the goaf.IntroductionOver the past two years several intensive air velocity surveys have been carried out in West Virginia coal mines as part of a programme aimed at understanding dust transport mechanisms on mechanized longwall faces. These are presented here and some of the patterns of velocity distribution are discussed.Ventilation survey methodologyThe air velocity vector consists of three mutually perpendicular subvectors; the vertical subvector is directed toward the roof, one of the horizontal subvectors is parallel to the coal face and the other pointing to the gob. The air velocity which was measured in this paper is the horizontal subvector parallel to the coal face.To record the velocity distributions at the longwall faces, the surveying stations and the surveying points were established as follows.(1) The first station usually was set at the third support from the headentry T-junction. Within the first few supports, the air coming from the intake airway encounters a sharp right-angle turn at the T-junction, and forms a turbulent region close to the coal face. The intake air in this region is also influenced by the motors that drive the face conveyer. But when the air reaches the different. But for the same face the number was the same. The main air-travelling area was divided into six to 12 equally sized sub-areas, and each surveying point was situated approximately at the centre of the sub-area.The velocity survey was carried out by two persons, one reading the meter and the other taking down the readings. A complete survey at one station took about 5-8 min. Usually, the dimension of the cross-sectional area was measured during that time.A third person used the smoke tubes to observe the following: (I) the air movement patterns at different locations; (2) the extent and the direction of the air leakage; and (3) the machines' influence on the main airflow. At the same time he observed and recorded the roof conditions and the compactness of the roof-caving in the gob."

Mar 1, 1989

Continuous remote sensing of air speed In underground mine airways. Approach An ultrasonic technique measures a form of air turbulence which is proportional to air velocity. How It Works The stainless-steel enclosed transducer consists of a sensing head with circuitry, and a telemetry card. The sensor uses a patented ultrasonic technique to measure a form of turbulence created In the air. This turbulence, known as vortex shedding, is related to the air speed through the sensor. Air flowing past a cylindrical post creates vortices which flow down the air stream on both sides of the post. Pulses from an ultrasonic transmitter pass through the vortices to a receiver; the effect of the vortices on the pulses is a measure of air velocity. The transducer contains a telemetry card, known as an accessor, which transmits reports on air velocity through a pair of wires to a monitoring system. The accessor contains an analog-to-digital converter and digital telemetry circuitry required for computer system operation. The transducer does not contain any read-outs or alarms and air velocities are displayed by means of a centrally located

Jan 1, 1982

By L. A. Schutz

Personnel of the Bureau of Mines have conducted investigations in the principal asbestos mines and mills in the United States, to determine the concentration of airborne asbestos fibers in the workplace, and to establish the exposure of workers to such fibers. 'The surveys were conducted using the sampling and evaluation method recommended by the National Institute for Occupational Safety and Health. The method consists of collecting the air-borne sample on filters and, after appropriate sample preparation, counting the fibers utilizing phase contrast microscopy. The results of the investigation show that fiber concentrations are low in the asbestos mines but high in the asbestos mills, ranging well above 5 fibers/ml of air based on a count of fibers greater than 5 urn in length.

Jan 1, 1973

By Douglas Pollock, John Organiscak

Water spray characteristics, including droplet size and velocity, airborne dust capture potential, and induced airflow quantity for various spray nozzle designs were evaluated to provide basic information for improving spray applications. Water droplet size and velocity characteristics were initially measured by a Phase Doppler Particle Analyzer (PDPA) for hollow cone, full cone, flat fan, and air atomized spray nozzles at similar operating parameters. Airflow inducement and dust capture experiments were also conducted under the same operating parameters to examine any salient features of the spray nozzle type, droplet characteristics, induced airflow, and airborne dust capture. Test results indicate that there are trade offs between airflow inducement and dust capture efficiency. A spray nozzle with a wider discharge angle was observed to induce more airflow, but at reduced dust capture efficiencies. Increasing spray nozzle fluid pressure(s) generally reduced water droplet sizes with concurrent increases in droplet velocity, airflow inducement, and airborne dust capture. Placing a three-sided barrier around the spray nozzles normally reduced spray air induction and increased dust capture efficiency. A direct relationship between airborne dust capture efficiency and spray input power normalized per unit of airflow induced was ob- served. This information can be utilized to improve the performance of water sprays for reducing airborne dust levels.

By Steven J. Page, John A. Organiscak

Airborne dust generation is one of the byproducts of coal mining, processing, and handling. The amount of airborne total dust (ATD, respirable size and larger) and airborne respirable dust (ARD) generated is of primary interest for designing the level of engineering controls needed for adequate dust abatement. Laboratory crushing experiments were conducted in a wind tunnel with a roll crusher to identify relationships among crushing parameters, product size, coal rank properties, and airborne dust generation. Through the first series of experiments, the effect of primary and secondary breakage processes on both product size and airborne dust generation was examined. Through a second series of experiments, the effect of coal rank properties on product size and airborne dust generation was studied using a uniform crushing process with secondary breakage. Laboratory results indicate that secondary breakage of a particular coal notably increases the specific amount of ATD generated, while negligibly impacting the specific amount of ARD generated. A strong positive relationship was identified between the specific amounts of ATD and ARD generated during the primary breakage process (with minimal secondary breakage), but a negligible relationship was observed between the same two variables when secondary breakage was introduced into the crushing process. This indicates that most of the ATD and ARD is generated from the primary breakage, while secondary breakage has a more of an influence on generating additional amounts of larger sized ATD. Additional experiments involving the uniform crushing of eight different bituminous coals showed that the coal rank, expressed as the inherent moist fuel ratio (MFR = fixed carbon =(volatile x inherent moisture)), had diverse relationships between the product size created and the amount of airborne dust generated. As bituminous coal rank or MFR increased, the amount of coal product fines < 250 gm increased while the mass percentage of ATD and ARD liberated from these < 250 µm product fines decreased. Air dry loss (ADL) moisture in the coal was found to be inversely related to the dust cloud electrostatic field, influencing dust liberation from the coal product fines. Since the MFR was directly related to the dust cloud electrostatic field, opposite the ADL-electrostatic field relationship, the diverse relationships between the product size created and the amount of dust liberated from the < 250 µm product fines were explained. Finally, 12 to 14 times more ATD was generated as compared to the ARD generated during these experiments. These results should provide a fundamental basis for initial estimation of the airborne dust loading requirements of engineering control methods for coal crushing.

By M. L. Wang, S. R. Short, E. M. Daniloff, E. L. Petsonk, D. M. Mannino, G .. R. Wagner

"Background—It has been suggested that health related job selection is a major cause of the healthy worker effect, and may result in inaccurate estimates of health risks of exposures in the working environment. Improved understanding of self selection, including the role of airway hyperresponsiveness, should improve accuracy in estimating occupational risks.Methods—We evaluated symptoms of the respiratory tract, lung function, occupa¬tional and smoking histories, and airway responsiveness from a cross sectional sur¬vey of 478 underground bituminous coal miners and non-mining controls. Workers with abnormal spirometry were excluded from methacholine testing.Results—Methacholine responsiveness (= 15% decline in forced expiratory volume in one second) was associated in both miners and controls with reduced ventilatory lung function and an increased risk of respiratory symptoms. Miners with the longest duration of work at the coal face had a low prevalence of methacholine responsiveness, compared with miners who had never worked at the coal face (12% v 39%, P < 0.01). Throughout their mining careers, miners who responded to methacholine were consistently less likely to have worked in dusty jobs than miners who did not respond to methacholine.Conclusions—These results provide evidence that workers who are employed in dusty jobs are less likely than their unexposed coworkers to show increased non-specific airway responsiveness, pre-sumably as a result of health related job selection. Surveys of workers in which responsiveness data are unavailable may underestimate the effects of dust exposure on respiratory health."

Dec 1, 1996

By R. L. Grayson, W. E. Wallace, M. D. Attfield, A. Q. Wearden, P. Bolsaitis, R. D. Kennedy, M. J. Keane, J. C. Harrison

"Respirable high-silica particles from four coal mines. a clay mine and a tunnelling site were analysed for silicon vs aluminum composition by performing scanning electron microscopy-energy dispersive X-ray analysis (SEM-EDS) at electron accelerating voltages of 20 kiloelectron volts (keV). The same particles were analysed at 5 keV to determine possible differences in surface vs bulk composition. In some cases high-silica particles exhibited a decrease in the ratio of silicon to aluminium K-alpha line intensities with decreasing electron beam accelerating voltage. This behaviour is consistent with aluminosilicate surface contamination or occlusion of a silica particle. Some 20 high-silica particles were analysed from each of 10 samples. Powdered aluminosilicate glass was used as a control for the behaviour of silica with a homogeneous distribution of aluminium in the particle. Significant differences were found between the control and sample means for an Illinois bituminous coal mine dust, two western and one central Pennsylvania bituminous coal mine dust low-temperature ashes. and a clay mine and mill dust sample, but not for an anthracite coal mine dust and two central Pennsylvania bituminous coal mine dust low-temperature ashes. INTRODUCTIONRESPIRABLE dust quartz content commonly is measured on bulk samples by using i.r. spectroscopy or X-ray diffraction. For research purposes, individual particle-by particle SEM-EDS analysis at 20-30 keV electron beam accelerating potential may be used also. Such measurements have not provided an unambiguous association of quartz content with occupational pulmonary disease prevalence in exposed workers (WALTON et al., 1977). Rather, there is evidence that coal rank modifies the relationship between dust exposure and pneumoconiosis development (WAL TON et al., 1977; ROBOCK and BAUER, 1990; ATTFIELD and MORRING, in press). SEM-EDS analysis was performed to measure the silicon fraction, Si/(Si +Ai), at 20 and at 5 keV on high-silica particles to determine if some respirable quartz particles generated by mining exhibit heterogenous composition with depth (WALLACE et al., 1990), which might affect the bioavailability of the quartz surface."

Dec 1, 1995

By S. J. Page, P. Bolsaitls, W. E. Wallace, C. A. Hill, M. J. Keane

"Boiling respirable quartz dust in water for 10 to 40 minutes decreases the cytotoxicity for both erythrocyte hemolytic potential and pulmonary macrophage release of lactate dehydrogenase in vitro. The potential is reduced to near zero in the boiling concentration range of 1 to 10 mg quartz per ml water in boiling times of 20 to 40 minutes. Below this range the detoxification occurs over a longer boiling time. Above this range the hemolytic potential remaining after 40 minutes of boiling approaches native quartz potential with increasing concentration up to 30 mg quartz per ml water. Replacing the media with fresh at the midpoint of boiling results in full detoxification through 20 mg per ml. Pre—boiling the medium with quartz or silica gel reduces the effect of subsequently boiling test samples. Detoxification persists after mild drying at 110 C for 8 hours, and persists after three days of resuspension in water at room temperature. Additional data on persistence of the effect, on the use of acidic and basic media treatment, and on surface analyses are presented, with a discussion of a working hypothesis and research needs.IntroductionResearch underway to determine interactions of quarts and other mineral dust surfaces with pulmonary fluids and alveolar macrophages in culture led to the observation that when dusts were autoclaved in aqueous suspension, their cytotoxic effects on macrophages wore suppressed, in some cases fully and even after several days incubation with the cells. This finding was in direct contradiction to earlier results from both short term macrophage lysosaal enzyme release assays, as well as longer term cytotoxicity assays from macrophages in culture; in those studies, dusts were steam autoclaved at 121 121 ºC with no liquid water but with steam present. (1,2) However detoxification under boiling conditions has been reported in other research.(3). It was decided to use the hemolysis assay to further investigate these findings, because of its sensitivity, simplicity and cost."

Jan 1, 1988

By William E. Wallace, S. J. Page, B. L. Razzaboni, C. A. Hill, Pamela Mike, P. Bolsaitis, M. J. Keane, Val Vallyathan

"Respirable quartz cytotoxicity, as measured by erythrocyte hemolysis and pulmonary macrophage release of lactate dehydrogenase in vitro, is neutralized by boiling in water in glass test tubes for 10 to 40 minutes. The cytotoxicity is reduced to near zero by boiling l to IO mg quartz per milliliter water. For greater concentrations of quartz in water the hemolytic potential after 40 minutes of boiling approaches that of native quartz. Replacing the medium with fresh water midway through boiling results in full detoxification through 20 mg quartz per milliliter water. Pre-boiling the medium with silica reduces the detoxification effect. Detoxification persists after mild drying at l 10°C for 8 hours, and persists after three days of resuspension in water at room temperature.INTRODCJCTIONResearch underway to determine interactions of quartz and other mineral dust surfaces with pulmonary fluids and alveolar macrophages in culture led to the observation that when dusts were autoclaved in aqueous suspension, their cytotoxic effects on macrophages were suppressed, in some cases fully and even after several days incubation with the cells. This finding was in direct contradiction to earlier results from both short term macrophage lysosmal enzyme release assays, as well as longer term cytotoxicity assays from macrophages in culture; in those studies, dusts were steam autoclaved at 121°C with no liquid water but with steam present. 1.2 However detoxification under boiling conditions has been reported in other research. 3 It was decided to use the hemolysis assay to further investigate these findings, because of its sensitivity simplicity and cost.RESCULTS AND DISCCISSIONRespirable quartz dust used in this study was taken from a stock of crystalline silica, Min-U-Sil; obtained from Pennsylvania Sand Glass Corporation, fractionated in air with a particle classifier. The small size fraction retained for use was 80% less than S micrometer particle diameter, with an area equivalent median diameter of 1.24 micrometers as estimated by automated image analysis. The silica was at least 98.S mass percent silica as determined by X-ray energy spectrometric analysis; and the crystalline form was alpha-quartz as determined by X-ray diffraction. It's specific surface area was 3.97 square meters per gram as determined by nitrogen adsorption isotherm methods. I"

Jan 1, 1990

By P. R. Miles, J. K. H. Ma, J. Y. C. Ma, L. Bowman

"The bisbenzylisoquinoline alkaloids are compounds which can be isolated from plants and which exhibit a broad range of pharmacological activities. Perhaps the best known alkaloid of this group is tetrandrine (TT), a compound isolated from the root of a traditional Chinese medicinal herb, Stephania tetrandra. It has been tested for its effects on the cardiovascular system, tumors, and the lungs. For example, tetrandrine has been shown to act as a calcium entry blocker in cardiac sarcolemmal vesicles (King et a/., 1988) and has been used in China as a treatment for angina and hypertension. Its possible effects as a tumor-inhibitory substance have also been investigated (Kupchan and Atland, 1973). In addition, there are several lines of evidence to suggest that it is a possible therapeutic agent for the treatment of silicosis. Yu et a/. (1983) have shown that TT inhibits the development of experimental silicosis in rats when it is administered orally after intratracheal instillation of quartz. Other studies have shown that the alkaloid inhibits the synthesis of collagen by fibroblasts and the formation of silicotic nodules in rats exposed to silica (Huang et al., 1981; Liu et al., 1983). In fact, tetrandrine has been used clinically in China as an antifibrotic agent and has been shown to improve both the appearance of chest radiographs and the measurements of diffusing capacity in patients with silicosis (Li et al., 1981).Since tetrandrine may have a potential use as a therapeutic agent for fibrotic lung diseases, its effects on various pulmonary cells and tissues should be studied. Alveolar type II cells are located in the alveolar regions of the lungs and are important because they are responsible for the synthesis and secretion of pulmonary surfactant (Dobbs et al., 1982; King, 1984), regeneration of the alveolar epithelium after lung injury (Kauffman et al., 1974), and metabolism of foreign compounds (Bend et al., 1985). Furthermore, in silicosis, for which TT has been suggested as a treatment, there are increased amounts of pulmonary surfactant produced (Dethloff et al., 1986a,b) as a result of increased rates of surfactant synthesis by type II cells (Dethloff et a/., 1989; Miller et al., 1988). Because type II cells play a critical role in maintaining normal lung function and because type II cells are affected in silicosis, for which 'TT has been used as a treatment, we studied the effects of tetrandrine on energy metabolism in these cells in vitro. Energy metabolism was chosen because it is a basic cellular function which is neces-sary for other cellular processes to occur. Since type II cells depend on mitochondrial metabolism for energy production (Fisher et al., 1980), we examined the effects of tetrandrine on cellular and mitochondrial ATP production and oxygen consumption. Additional experiments were performed in an effort to determine if intracellular calcium levels may be involved in the TT-induced effects. A preliminary report of those results has appeared previously (Ma et al., 1991)."

Nov 1, 1995

By D. E. Banks, J. K. H. Ma, Y. Rojanasakul, J. Y. C. Ma, C. J. Malanga

There is evidence to suggest that cell injury induced in alveolar macrophages (AM) following phagocytic activation by silica particles may be mediated through changes in intracellular free calcium [Ca 2+}. However, the mechanism of silicainduced cytotoxicity relative to [Ca2+], overloading is not yet clear. To provide a better insight into this mechanism, isolated rat AMs were exposed to varying concentrations of crystalline silica (particle size< 5 µm in diameter) and the fluctuation in their [Ca2+]; and cell integrity were quantitatively monitored with the fluorescent calcium probe, Fura-2 AM, and the membrane integrity indicator, propidium iodide (Pl). Results from this study indicate that silica can rapidly increase [Ca2+]; in a dose-dependent manner with a characteristic transient calcium rise at low doses (<0.1 mg/ml) and an elevated and sustained rise at high doses (>0.1 mg/ml). Depletion of extracellular calcium [Ca 2+]0 markedly inhibited the [Ca2+]; rise (˜90%), suggesting that Ca2+ influx from extracellular source is a major mechanism for silica-induced [Ca2+]; rise. When used at low doses but sufficient to cause a transient [Ca2+]; rise, silica did not cause significant increase in cellular Pl uptake during the time of study, suggesting the presevation of membrane integrity of AMs under these conditions. At high doses of silica, however, a marked increase in Pl nuclear fluorescence was observed. Depletion of [Ca 2+] 0 greatly inhibited cellular Pl uptake, induced by 0. 1 mg/ml or higher doses of silica. This suggests that Ca 2 + influx, as a result of silica activation, is associated with cell injury. Indeed, our results further demonstrated that the low dose effect of silica on Ca 2+ influx is inhibited by the Ca2+ channel blocker nifedipine. At high doses of silica (>0.1 mg/ml), cell injury was not prevented by nifedipine or extracellular Ca2+ depletion, suggesting that other cytotoxic mechanisms, i.e., nonspecific membrane damage due to lipid peroxidation, are also responsible for the silica-induced cell injury. Silica had no significant effect on cellular ATP content during the time course of the study, indicating that the observed silicainduced (Ca2 ... ); rise was not due to the impairment of Ca2 + -ATPase pumps, which restricts Ca2+ efflux. Pretreatment of the cells with cytochalasin B to block phagocytosis failed to prevent the effect of silica on [Ca2+]; rise. Taken together, these results suggest that the elevation of [Ca2+]; caused by silica is due mainly to Ca2+ influx through plasma membrane Ca2+,channels and nonspecific membrane damage (at high doses). Neither ATP depletion nor Ca2+ leakage during phagocytosis was attributed to the silica-induced [Ca2+]; rise.

Nov 1, 1995

By Michael J. Sapko, Samuel P. Harteis, Eric S. Weiss

The Pittsburgh Research Laboratory (PRL) of the National Institute for Occupational Safety and Health (NIOSH) recently conducted full-scale explosion experiments; these experiments evaluated the strength characteristics of various seal designs used for safely isolating worked-out areas in underground coal mines. Large-scale explosion tests were conducted within the multiple entry section of PRL’s Lake Lynn Experimental Mine (LLEM) employing the only currently accepted test method endorsed by the Mine Safety and Health Administration (MSHA) for seal design in American mines. These explosion tests are labor-intensive, expensive to conduct, and can interfere with other critical underground safety and health research programs conducted by NIOSH. Therefore, the PRL has developed an alternative seal evaluation method, based on a hydrostatic pressure loading concept, that can facilitate the in-situ testing of seals in an operating mine. Two chambers within LLEM and one within the Safety Research Coal Mine (SRCM) were used for hydrostatic pressure loading various seal designs. The results from tests in these chambers compare favorably with those from the large-scale explosion tests in the multiple entries. Preliminary size-scaling relationships for predicting the strength of standard seal designs as a function of entry size is also presented. In addition to testing seal designs at the required 20 psi static pressure level, the new facilities also allow for the determination of the seal’s ultimate failure pressure. This new approach shows promise as an alternative evaluation method for improving mine sealing technologies which, in turn, will enhance the safety of the underground personnel.

By Errol D. Sehnke

This U.S. Bureau of Mines report provides detailed information on worldwide alumina production capacity by plant and country. The information was compiled from a variety of domestic and international sources and was based on information available to the Bureau through December 31, 1993. Capacity data represent announced installed and planned capacities and do not necessarily reflect a Bureau of Mines judgment of actual plant operating levels or timing of planned expansions, The year-end capacity is the ?nameplate" capacity or estimated production capacity, whichever is larger.

Jan 1, 1993

Table 1.-Salient aluminum statistics (Thousand short tons and thousand dollars) [ ]

Jan 1, 1967

By D. Eugene Rannels, Yu-Chen Lee, John W. Swisher

"Inhaled dust particles of appropriate size contact cells of the alveolar region.' The acute response to coal dust appears to involve a variety of cell types, including alveolar epithelial cells, interstitial fibroblasts, both resident and recruited macrophages, and cells of the immune system. Direct and indirect effects of dust, which may be mediated by soluble factors,'' can initiate acute and long-term lung injury. While it appears that epithelial cell growth and differentiation, along with integrity of the epithelial basement membrane, may play a role in the long-term response to dust exposure, little is known of the role the alveolar epithelium may play in the progression of injury. The broad hypothesis that underlies these experiments is that short- and long-term consequences of coal dust exposure involve changes in structure and function of the alveolar epithelium and the underlying extracellular matrix. Recent studies show that coal dusts exert direct effects to modify pulmonary epithelial cell-extracellular matrix interactions in vitro.3EXPERIMENTAL PROCEDURESType II cells isolated on day 0 by density centrifugation and differential attachment4 were plated overnight in D MEM containing 10% fetal calf serum before exposure to anthracite dust PSOC 867 sonicated into culture medium (750 ug/mL).3 Synthesis of extracellular matrix proteins was determined on day 3 of primary culture, as detailed elsewhere. 5 Cells were removed from extracellular matrix by extraction with NH4OH and high salt. Extracellular matrix synthesis and fibronectin content were evaluated based on radiolabeling with 3 H-leucine and on Western blot analysis, respectively. Additional analytical methods and calculations are detailed elsewhere.3-5"

Jan 1, 1998

By D. Eugene Rannels, Yu-Chen Lee

"Lee, Yu-Chen, and D. Eugene Rannels. Alveolar macrophages modulate the epithelial cell response to coal dust in vitro. Am. J. Physicl. 270 (Lung Cell. Mol. Physiol. 14): L123-L132, 1996.-The response of the alveolar epithelium to coal dust exposure is poorly understood. Coal or other dusts may act on the epithelium directly or indirectly through nearby alveolar macrophages (AM) that produce cytokines and other soluble products. AM and type II pneumocytes (T2P) were thus exposed to dust in coculture to evaluate their possible interactions. Anthracite coal dust PSOC 867 increased synthesis of extracellular matrix (ECM) components by T2P. AM alone did not produce ECM. Similarly, coculture ofT2P with AM (3.75:1) had little effect on epithelial ECM synthesis. In contrast, coculture ofT2P with AM significantly increased PSOC 867 effects on T2P rates of ECM synthesis, ECM fibronectin content, and T2P levels of fibronectin mRNA. AM-conditioned medium did not change the PSOC 867 effect on T2P. Neither control nor PSOC 867-treated AM on Falcon culture inserts (0.45-µm pore size) over T2P stimulated ECM synthesis by either untreated or dust-exposed epithelium. Thus AM-mediated changes in ECM synthesis by PSOC 867-treated T2P require close cell-cell interactions, suggesting a role for cell-cell contact or for short-lived soluble mediators of the AM effects. DEVELOPMENT OF PNEUMOCONIOSIS, a chronic disease of coal workers that results in pulmonary fibrosis and low lung compliance, is associated with inhalation of mineral or coal dust in the work place (14). Although the pathology of pneumoconiosis has been studied extensively, cellular mechanisms that cause changes in lung structure and function are not fully understood. Particulate matter that enters the respiratory system distributes according to size, with larger particles being removed in proximal airways. Dust components of small size may enter distal alveolar region, which is populated by two types of epithelial cells, type I and - type II cells, and by alveolar macrophages (16). Each of these cell types may interact with inhaled particles, but only the effects of coal dust on alveolar macrophages have been studied extensively. Less is known regarding the response of the alveolar epithelium, or of effects on epithelial cell-alveolar macrophage interactions, in the dust-exposed lung.Function of the alveolar epithelium is well known to be influenced by the underlying basement membrane, or extracellular matrix (28). Alveolar epithelial cell extracellular matrix interactions, which may involve modulation of cell migration, attachment, or differentiation (17), are reflected in type II cell morphology, metabolism, secretory activity, hormone responsiveness, lamellar body content, and gene expression (21). The extent to which basement membrane modulates differentiation of the overlying epithelium is determined by both molecular composition and three-dimensional structure of the matrix. Type II epithelial cells in vitro synthesize, secrete, assemble, and degrade a variety of basement membrane components, including laminin, fibronectin, type IV collagen, and thrombospondin (22, 27). How expression of these components is regulated, directly or indirectly, upon exposure of the alveolar epithelium to injury by coal dust or by other particulates remains to be resolved."

Jan 1, 1998

By W. A. Selvig

EQUIREMENTS of montan wax for industrial use in the United States before World War II were met by imports, nearly all of which came from Germany. An important industry for extraction and refinement of this wax from certain brown coals had developed in Germany. At the beginning of the war, montan-wax imports were shut oft, and American industry was forced to rely largely on substitutes. Since the war, relatively small amounts have been imported from Czechoslovakia. Because of the shortage of montan wax at the close of the war and the fact that industry in the United States had to rely on foreign sources in the past, this investigation was started in 1945 to determine probable yields of wax from domestic lignite. Results of the investigation and the properties of extracted waxes are described in this report. Also included in the report are discussions of the occurrence and geology of lignite deposits of the United States from which samples were obtained for wax extraction and the petrographic composition of lignites, particularly in respect to those components that contribute to wax yields.

Jan 1, 1950

By M. Kanda, J. W. Adams

A system for measuring amplitude probability distributions (APD’s) of electromagnetic noise in coal mines is described and typical APD’s from an underground coal mine are presented. The APD is a basic statistic required for the design and analysis of communication systems, especially those intended for use in noisy environments, and where neither overdesign nor underdesign is acceptable. The rms and average field strengths are obtained by integration of the APD, and examples are shown at several frequencies. All field strength levels are given in absolute units. Selected frequencies cover the range from 10 kHz to 32 MHz.

Jan 1, 1973