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By N. Pradhan, B. K. Mishra, B. D. Nayak, B. K. Mohapatra, R. K. Mohapatra

"Present study is about use of a group of Iron[Fe(III)] Reducing Bacteria (IRB) to convert the goethite (a-FeOOH) present in the original lateritic Nickel ore to magnetite under an anaerobic condition and subsequently release the bound Co(III) and Ni(II) through leaching. The lateritic Nickel ore contains 0.8% Ni, 0.049% Co, 1.92% Cr, 0.32% Mn and 50.2% Fe. An anaerobic dissimilatory Fe(III) reducing bacterial consortium capable of using acetate as carbon source (electron donor) and lateritic ore as terminal electron acceptor, changes the initial light brown color of the ore to dark brown. The change in color is due to the conversion of goethite to magnetite, which was confirmed by XRD. When the IRB treated sample was subjected to both bioleaching and acid leaching, it shows a greater recovery of Nickel and Cobalt values as compared to untreated original lateritic Ni ore. Further a magnetic separation method was used to separate the magnetic part of the treated lateritic ore and subjected to acid- and bio-leaching for better recovery of Nickel and Cobalt.IntroductionIt has been known that micro-organisms have the potential to reduce metals but more recent observations have shown that a diversity of specialist bacteria and archaea can use such activities to conserve energy for growth under anaerobic conditions [12]. Natural Fe(III) oxides are high in surface area and are reactive [1]. Fe (III) oxides adsorb a wide range of metal cations and anions by complexation to surface hydroxyl groups [17], and function as the primary redox buffering solid phase in many sediments and subsurface materials [5].Recently a number of bacteria have been isolated based on their ability to use metal ions, including Fe(III), as electron acceptor under anaerobic conditions. These are capable of producing energy by coupling the oxidation of organic compounds to reduction of Fe(III). Iron[Fe(III)]-Reducing Bacteria (IRB) have been isolated and identified from a wide variety of environments including freshwater and marine aquatic sediments and submerged soil [8]. The biogeochemical cycle of iron is linked to those of the trace metals, as many trace metals coassociate with Fe(III) oxides or reduced iron phases (e.g., magnetite, siderite, or iron sulfide). IRB can utilize Fe(III) oxides as terminal electron acceptors for respiration [18]; thereby reducing all or a fraction of the solid. The reductive portion of the iron biogeochemical cycle in non-phototropic environments (e.g., sediments and subsurface materials) is driven by the direct enzymatic reduction of Fe(III) oxides to Fe(II) by dissimilatory iron reducing bacteria [13,14]."

Jan 1, 2009

By H. Y. Sun, Q. Y. Tan, X. P. Niu, L. Li, Y. Jia, R. M. Ruan

During bioleaching processes of sulfide minerals, the sulfide minerals act as the natural habitats for acidophiles, of which, most are autotrophic iron and sulfur oxidizing microbes. These microbes acquire energy by oxidation of sulfide minerals, and play crucial role in bioleaching processes. In the industrial bioleaching heaps, microbial community and their activity are highly related to engineering configurations, while the optimized industrial operation can help to fast dissolution of aim minerals and acid/iron balance under optimized microbial condition. Microbial community succession and activity formation, the function of microbes to mineral dissolution, the mechanisms to promotion and inhibition of sulfide minerals oxidation, and the industrial instances for microbial community regulation were reviewed. For these instances, promoted microbial activity helped to fast pyrite oxidation for sulfuric acid generation, which realized zero sulfur acid addition to the irrigation solution in the initiation of a bioleaching heaps. High microbial activity by optimized operation parameters improved Cu leaching efficiency during the heap bioleaching process. And also by inhibition of microbial activity, especially the iron oxidizing microbes, the excessive pyrite oxidation can be inhibited, which helped to the acid/iron balance during heap bioleaching. INTRODUCTION Around the world, high grade copper ore are exploited, while barely new high-grade deposits are succeeded, the copper grade of ore deposit is constantly decreasing. The increasing copper demands worldwide requests to process these low-grade ore deposits. Processing of low-grade ores are not economical by usual extraction processes. Heap leaching is a cheaper process for recovery of low-grade ores, and has been applied to extract valuable metals from sulfide minerals worldwide, such as copper, gold, uranium and nickel (Gericke et al., 2009). Heap leaching process has greatly decreased the economic grade and expanded the recoverable ore reserve worldwide (Brierley, 2008). It is usually carried out in the open environments, and could process huge amount of ore (usually in millions of tonnes in one reactor) in the heap reactor (Gericke et al., 2009). For copper sulfide, especially the secondary copper sulfides, heap (dump) bioleaching is successfully applied. Heap bioleaching process is cost saving, and sometimes can be achieved without adding chemical into heaps, by microbial fixing of the atmospheric N2 and CO2, and generating oxidant Fe3+ and sulfuric acid in heaps from oxidation of sulfide minerals. Also heap bioleaching is environment friendly by cycling use of the leaching solution, and without exhaust emission.

Jan 1, 2019

By B, R E. Browner, H R. Watling

A number of hydrometallurgical treatment methods are being developed to extract copper from chalcopyrite flotation concentrate including microbial leaching and ferric sulfate leaching. However, chalcopyrite is difficult to process efficiently by hydrometallurgical methods as it exhibits slow reaction kinetics. Comparative leaching tests do not always satisfactorily explain or predict observed copper recovery, acid consumption, or differential leaching of minerals. This is especially true when reactive gangue constituents are present. The aim of this study was to determine how the leaching regime (microbial versus ferric sulfate) affects both the extraction of copper and residue mineralogy. Chalcopyrite flotation concentrate was leached at 30¦C and 65¦C using a pure culture of Acidithiobacillus ferrooxidans and a mixed consortium of thermophiles, respectively. In addition, the concentrate was leached at the same temperatures using 0.1 M ferric sulfate. Microbial leaching of chalcopyrite extracted more copper than ferric sulfate oxidation of chalcopyrite at 30¦C, demonstrating that the microbes promoted the solubilisation of copper at this temperature. This result was confirmed by leach solution analyses and residue analysis using quantitative X-ray diffraction (QXRD). More chalcopyrite was oxidized with ferric sulfate at 65¦C than at 30¦C (as expected). QXRD confirmed that pyrite reacted during ferric sulfate leaching at 65¦C but was relatively unreactive during lower temperature leaching. The high ferric ion concentration in the ferric sulfate leach at 65¦C led to significant precipitation of jarosite. This phase was only detected in small amounts at 30¦C in both the microbial and ferric leached residues. Sulfur also precipitated in the ferric leaching at 65¦C whereas no sulfur was detected in the microbial leached residue. Quartz was a relatively unreactive mineral phase regardless of the leaching medium and temperature. QXRD analysis indicated the residue mineralogy differed with different leaching regime.

Jan 1, 2004

By H. Sarvamangala

Selective separation of alumina, silica, calcite and apatite from hematite was achieved through microbiologically induced flotation and flocculation in presence of bacteria (B.subtilis) and that of hematite from apatite after interaction with yeast (Saccharomyces cerevisiae). Bacterial and yeast metabolites containing extracellular proteins were characterized from mineral-grown cell free extract. Bacteria and yeast can adhere to mineral surfaces and influence subsequent separation of the minerals. Bacteria functioned as a stronger depressant for hematite, while yeast cells depressed apatite at neutral pH. Selective affinity of the bacterial and yeast cells towards the mineral surface was observed through adsorption studies. Extracellular proteins (EP) were isolated from B.subtilis and yeast. The protein profile of the EP of bacterial and yeast cells grown in presence and absence of minerals was studied. This study has demonstrated the utility and amenability of microbially-induced mineral beneficiation of iron ores through bacterial and yeast cells and their metabolic by products.

Sep 1, 2012

By K. A. Natarajan

"Interaction with microorganisms results in significant surface chemical changes on minerals. For example, chemolithotrophs such as Thiobacillus ferrooxidans and Thiobacillus thiooxidans significantly alter the surface chemistry of several sulfide minerals such as pyrite, chalcopyrite, sphalerite and galena. Heterotrophic bacteria such as Bacillus sp. could alter the electrokinetic properties of oxide minerals such as hematite, alumina, silica, kaolinite and calcite. Microbe-mineral interactions could thus be beneficially utilised in mineral beneficiation as different from bioleaching. Microbially induced mineral beneficiation unlike bioleaching deals with interfacial phenomena at mineral-solution-bacteria interfaces and surface chemical changes are brought about at very rapid rates amounting to a few minutes resulting in mineral separations in an aqueous medium.In this paper, the role of acidophilic chemolithotrophs such as Thiobacillus thiooxidans and neutrophilic heterotrophs such as Bacillus polymyxa in the beneficiation of sulfide and oxide minerals is illustrated. Mechanisms of bacterial attachment on various sulfide and oxide minerals are outlined with respect to the influence of bacterial interaction in bringing about mineral surface hydrophobicity or hydrophilicity. The role of bacterial cells as well as metabolic products such as bioproteins and exopolysaccharides in significantly altering the surface chemistry of sulfide minerals such as sphalerite and galena and oxides such as hematite, alumina, silica and calcite is discussed with probable mechanisms. Possibilities of generating mineralspecific bioreagents are outlined with respect to bacterial adaptation to mineral substrates."

Jan 1, 2003

By K. A. Natarajan

Interaction with microorganisms results in significant surface chemical changes on minerals. For example, chemolithotrophs such as Thiobacillus ferrooxidans and Thiobacillus thiooxidans significantly alter the surface chemistry of several sulfide minerals such as pyrite, chalcopyrite, sphalerite and galena. Heterotrophic bacteria such as Bacillus sp. could alter the electrokinetic properties of oxide minerals such as hematite, alumina, silica, kaolinite and calcite. Microbe-mineral interactions could thus be beneficially utilised in mineral beneficiation as different from bioleaching. Microbially induced mineral beneficiation unlike bioleaching deals with interfacial phenomena at mineral-solution-bacteria interfaces and surface chemical changes are brought about at very rapid rates amounting to a few minutes resulting in mineral separations in an aqueous medium.

Jan 1, 2003

By K. A. Natarajan, M. N. Chandraprabha

Selective separation of minerals from complex sulphide ores is a challenging task and it is often difficult to get commercially acceptable individual concentrates by conventional selective flotation using inorganic collector reagents. Microbially-induced mineral beneficiation has proved to be more effective, economically viable and an environmentally benign process for the effective separation of various minerals. Applications of microbially-induced mineral beneficiation are demonstrated in this paper with respect to beneficiation of complex multimetal sulfides. The role of adapted microorganisms and bioreagents in the beneficiation of complex sulphide minerals are also illustrated.

Jan 1, 2009

By M. N. Chandraprabha

This paper discusses the role of the mineral-adapted acidiphilic microorganism Acidithiobacillus ferrooxidans in the beneficiation of arsenopyrite-containing multisulfides (pyrite and chalcopyrite) and the bioremediation of the resulting arsenical waste water. It was found that adaptation to minerals alters the surface properties of the microorganism. Bacterial adaptation to arsenopyrite and controlled bacterial adhesion to mineral surfaces lead to selectivity in arsenopyrite separation. Bioremoval of arsenic ions (both arsenite and arsenate ions) by Acidithiobacillus ferrooxidans is also discussed. Key words: Acidithiobacillus ferrooxidans, Bioremediation, Arsenopyrite beneficiation

Jan 1, 2009

By K. A. Natarajan

The utility of yeast, Saccharomyces cerevisiae, in the separation of quartz from hematite is demonstrated. Yeast cells, as well as their metabolites, functioned as flotation collectors, depressants or flocculants and dispersants for hematite and quartz. Interaction between yeast and the above minerals resulted in significant surface chemical changes, rendering quartz surfaces hydrophobic and hematite hydrophilic. Mineral-specific extracellular proteins and exopolysaccharides were secreted by yeast cells when grown in the presence of quartz and hematite, respectively. Quartz could be efficiently separated from hematite through microbially induced flotation and selective flocculation.

May 1, 2012

By J. H. Reiss, D. F. Bruhn, Idaho National Laboratory, Y. Jiao, D. W. Reed, Y. Fujita, D. L. Daubaras

"Bioleaching offers a potential approach for recovery of rare earth elements (REE) from recyclable materials, such as fluorescent lamp phosphors or degraded industrial catalysts. Microorganisms were enriched from REE-containing ores and recyclable materials with the goal of identifying strains capable of extracting REE from solid materials. Over 100 heterotrophic microorganisms were isolated and screened for their ability to produce organic acids capable of leaching REE. The ten most promising isolates were most closely related to Pseudomonas, Acinetobacter and Talaromyces. Of the acids produced, gluconic acid appeared to be the most effective at leaching REE (yttrium, lanthanum, cerium, europium, and terbium) from retorted phosphor powders (RPP), fluidized cracking catalyst (FCC), and europium-doped yttrium oxide (YOEu). The Acinetobacter isolate, BH1, was the most capable strain and able to leach 33% of the total REE content from the FCC material. These results support the continuing evaluation of gluconic acid-producing microbes for large-scale REE recovery from recyclable materials.INTRODUCTIONAn enhanced recovery, substitute, reuse or recycle paradigm is important for balancing the supply and demand of critical materials (DOE, 2011). The recovery of rare earth elements (REE) from recyclable materials is an important component in this model. REE are used in a variety of modern products. For example, neodymium, europium, terbium, dysprosium, and yttrium are important for applications such as permanent magnets, lamp phosphors, rechargeable batteries, etc. (Binnemans, Jones, Blanpain, Van Gerven, Yang, Walton, & Buchert, 2013; DOE, 2011; Graedel, Harper, Nassar, & Reck, 2013). Cerium and lanthanum are used in glass polishers and additives in fluidized cracking catalysts (FCC)."

Jan 1, 2016

By M. N. Chandraprabha

This paper discusses the role of mineral-adapted acidiphilic microorganism Acidithiobacillus ferrooxidans on the beneficiation of arsenopyrite containing multisulfides (pyrite and chalcopyrite). Adaptation to minerals altered the surface properties of the microorganism. Bacterial adaptation to arsenopyrite and controlled bacterial adhesion to mineral surfaces lead to selectivity in arsenopyrite separation. Bioremoval of arsenic ions (both arsenite and arsenate ions) by Acidithiobacillus ferrooxidans is also discussed.

Jan 1, 2009

By W. Douglas Gould, Mei-Lin K. Stichbury, Jeffrey G. Bain, Blowes David W.

The mechanisms of release of arsenic in gold mine tailings were investigated. The highest concentration of dissolved As (75 mg/L) was observed near the base of the tailings impoundment, in close association with a peat layer. Elevated concentrations of reduced iron and hydrogen sulfide were also measured at the base of the impoundment. High numbers of iron-reducing and sulfate-reducing bacteria were detected at depths within the impoundment which correlated with dissolved As, Fe and H2S. The data presented in this paper suggest that arsenic is released from the tailings by bacterially-mediated reductive dissolution of As-bearing iron minerals.

Jan 1, 2000

By J. A. Muñoz, A. Ballester, C. Gómez, M. E. Sevillano, M. Figueroa

The electrochemical behavior of massive chalcopyrite (CuFeS2) electrodes has been studied in the absence and-in the presence of mesophilic microorganisms, using cyclic voltametry as analytical technique. The culture was principally formed for Thiobacillus ferrooxidans, Thiobacillus thiooxidans and Leptospirillum ferrooxidans. The results showed that the reactivity of the chalcopyrite gradually increased with the bacterial treatment. The anodic dissolution process of the chalcopyrite treated with microorganisms was related to the breaking of passive layers when the applied potential increased. The analysis of the surface products showed that the chalcopyrite was covered with a superficial film avoided its dissolution during the bioleaching test. So, the rest potential of the bioleached samples increased possibly as consequence of its superficial transformation.

Jan 1, 1995

By Madgwick J, Pracejus B

The microbial, microaerobic, biodegradation of cryptomelane-rich and pyrolusite-rich samples of manganese oxides was studied in repeated five-day static batch cultures over II weeks. Manganous ion was leached/reduced faster from the cryptomelane-rich samples (average 510 mg Mn2+/1/d) than from the pyrolusite samples (average 442 mg Mn2+/1/d).Cultures contained 20 per cent (w/vl) manganese mude, five per cent (w/v) molasses nutrient and the end-of-batch bacterial cell numbers were approximately 1010 cells/ml. The cryplomelane-rich sample incubates became more acid with time than the pyrolusite-rich incubates. The breakdown of pyrolusite proceeds in a relatively uniform way, while cryplomelane indicates a stepwise destabilisation.

Jan 1, 1992

By A. Bruynesteyn

I would like to talk to you today about microbiological leaching or, the solubilization of metal values due to the action of Thiobacillus ferrooxidans. Bacterial attack of sulfide minerals has been going on for centuries but only recently was it discovered that the acid and iron often found in coal mine drainage is produced by bacterial action on the iron sulfides in the coals. Not until 1950 was it proven that bacteria were playing a role in the dump leaching of sulfide ores. B. C. Research became involved in microbiological leaching in 1955 while examining mine drainage from the copper-zinc mine at Britannia Beach, north of Vancouver. Since 1955, B.C. Research has maintained a continuous re¬search program on microbiological leaching which, due to its applied nature, was put under industrial sponsorship in 1962. Most of the information pro¬vided in my talk today has been gathered under this sponsored research program and I would like to express our appreciation to the many sponsors who have made our work possible.

Jan 1, 1970

By Sudhir C. Dhara

An attempt has been made on the concentration of Platinum Group Metals (PGM) from the converter matte material by microbial .leaching. Biological oxidation of base metal sulfides such as iron, copper, nickel and free sulfur present in the matte material using Thiobacillus ferrooxidans is an alternative method to concentrate the PGM-and gold. The method which produces a platinum group metals concentrate is comparable to chemical processes and electrochemical process.

Jan 1, 1995

By O. H. Tuovinen, T. M. Bhatti

Microbiological leaching has been used as an alternative approach to conventional hydrometallurgical methods of uranium extraction. In the microbiological leaching process, iron-oxidizing bacteria oxidize pyritic phases to ferric iron and sulfuric acid, and uranium is solubilized from the ore due to sulfuric acid attack. If uranium in the ore material is in the reduced, tetravalent form (UIV), a red ox reaction is involved whereby uranium is oxidized to the hexavalent form (UvI) upon dissolution. In acid-leaching systems, the primary oxidant is ferric iron, which is reduced to ferrous iron by its chemical reaction with UIV. The ferrous iron thus formed is reoxidized to ferric iron by iron-oxidizing bacteria such as Thiobacillus ferrooxidans and Leptospirillum ferrooxidans. Nutritional requirements and responses to environmental extremes of acidophilic iron-oxidizing bacteria are appraised. The S-entity in Fe-sulfides is oxidized to sulfate by bacteria similar to Thiobacillus ferrooxidans and Thiobacillus thiooxidans. Pyrite and marcasite oxidation is a sulfuric acid forming reaction. Heap, dump and in situ leach techniques are feasible as bacterial leaching systems.

Jan 1, 2000

By James A. Brierley, Corale L. Brierley

Microorganisms have been considered as agents which may be of importance in catalyzing hydrometallurgical processes for the extraction of metals from low-grade ore minerals. Various microorganisms and their role in bacterial leaching are described. Bacterial leaching as applied to copper, uranium, zinc, lead, and coal is discussed.

Jan 1, 1981

By Lawson E, Purkiss S. A R, Van Ashwegen p C

It is only in the past decade or so that industrial exploitation of biological resources and techniques has become regarded as a distinct discipline. Commonly referred to as Biotechnology, it embraces biochemistry, microbiology and the engineering sciences and is mainly aimed at the biological conversion of suitable raw materials into desired end products. The catalytic agents are more often than not bacteria although other micro-organisms such as fungi are used. The range of micro-organisms used is wide and the choice depends upon the desired end product, which in turn depends upon the metabolic attributes, genetically engineered or otherwise, of the cells. The metabolic diversity of micro-organisms has been applied to processes such as antibiotic production, food processing, brewing and chemical production, and of course extraction of metals from ores. Metabolic diversity is, to a certain extent, based on nutrition, which, in its simplest form, can be divided into energy source, carbon requirements and nature of the electron donor producing reducing power to the cells. The source of energy may be light, in which case the organisms are phototrophic (eg green plants, algae). The carbon requirements and reducing power may come from organic sources. These organisms are then called heterotrophic (eg man and most bacteria, including the well known Escherichia colt). However, there is a group of bacteria whose energy metabolism depends upon chemical redox reactions, use inorganic carbon (CO2) and obtain their reducing power from reduced chemicals such as metal sulphides. These are known as chemolithotrophs. They play an important role in mineral recycling in nature and are also ideal for extraction of metals from ores. This is because they are extremeophiles being acidophilic (grow at very low pH) and sometimes also thermophilic (grow at very high temperatures). These bacteria operate in such hostile environments that competition, or contamination, by other organisms does not occur. Extraction of metals from ores by chemolithotrophic bacteria is commonly referred to as biohydrometallurgy. Other related areas are bioadsorption of metals from dilute solution,- microbial treatment of metal contaminated wastes and removal of toxic chemicals produced or used during mineral processing. This paper basically reviews the work that Gencor has undertaken in the field of biohydrometallurgy and the advancements it has made in commercialising the technology, but also touches on certain long-term areas of research that are currently under investigation.

Jan 1, 1995

By J J. Plumb, P D. Franzmann

A study was conducted to describe the microbiology of the MICCL Monywa chalcocite heap bioleaching operation. Microorganisms were detected in the heap using culture-based techniques and cultureindependent PCR-DGGE analysis of the 16S rRNA gene. The metabolic requirements, temperature and pH optima were determined for selected strains isolated from the heap material. Six strains (BH1-BH6) were enriched and isolated from heap solids and leachate samples. Phylogenetic analysis of the 16S rRNA genes showed that strains BH3 and BH4 were closely related to the acidophilic bacterium Acidithiobacillus caldus and strains BH5 and BH6 were closely related to Leptospirillum ferriphilum. The optimum growth temperature for L. ferriphilum was 41.3¦C and the optimum pH range was 1.1 to 1.5. A new species of Archaea was isolated from the heap samples. Cells of the proposed species, Ferroplasma cyprexacervatum (æcyprusÆ L.n. meaning copper; æexacervoÆ L.v. meaning æto heap upÆ), are non-motile pleomorphic cocci, capable of chemomixotrophic growth with ferrous sulfate and yeast extract. Cells grow anaerobically on ferric iron in the presence of potassium tetrathionate and yeast extract as electron donors. Growth occurs from 14¦C to 63¦C, with an optimum temperature of 55.2¦C. The optimal pH for growth was 1.0 - 1.2. The species was phylogenetically most closely related to Ferroplasma acidiphilum with a 16S rRNA gene sequence similarity of 95 per cent. Molecular analysis of mine samples (16S rRNA PCR-DGGE) detected the above-mentioned cultured strains of microorganisms as well as species of Sulfobacillus and other unknown Proteobacteria. The microbial community of the Myanmar bioleach heaps contained similar levels of diversity to a dump bioleach operation in Chile, but contained greater amounts of biomass than another heap bioleach operation in Australia. The operating parameters of the Myanmar heaps (low pH and moderate temperature) have selected for the growth of moderately thermophilic microorganisms that are more commonly found in extremely low pH acidic mine drainage sites than in heap systems. The novel species of Ferroplasma is thought to be involved in the aerobic and anaerobic cycling of iron within the heap bioleaching environment.

Jan 1, 2004