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By M. Okido, O. Takai, Z. Zhao, M. Ishikawa, R. Ichino, K. Kuroda

"Electrolytic method was applied to calcium phosphate coating on titanium plates as an excellent technique for high density and intensive contact of coated films on biomaterials. The effect of H2O 2 as oxidation reagent on coating characteristics was examined to obtain biomineral films. Solution was containing 7.0 X 10·3 mol/dm3 ofCaC12 and 3.0 X 10·2 of Ca(H2PO4)z, kept at 55 ""C. All deposits were hydroxyapatite (HAp) in this study, independent of H2O 2 concentration, applied cathodic potential and solution pH. In the case of H2O 2 addition, an increase in cathode current started at the low potential, namely, ca. -0.2 V (vs. SCE). In contrast, the current increased at more than ca. -1.4 V in the solution without H2O 2 addition. H2O 2 appeared to be an effective reagent for increasing nucleation density and improving adhesion of deposits on titanium plates.IntroductionIn recent years, many researches have been undertaken to develop the techniques of calcium phosphate coating on titanium or bioglass for medical implantation. High density and intensive adhesion of coating are necessary for adding bioaffinity to these materials and for inducing bone morphogenesis on them when implanted in human body.1) Electrolytic deposition has been a newly developed method as high-speed deposition process. By this method, the supersaturation of calcium phosphate can be increased just in the vicinity of metal substrates and the heterogeneous nucleation is promoted on them. In this process, H2O is reduced according to eq. (1) on the cathodically polarized electrode"

Jan 1, 2000

By Abhilash

Uranium is conventionally extracted using a process that employs strong acid (H2SO4) and an oxidant (MnO2) as leaching agents. This method often creates environmental problems, requires large amounts of energy, and involves a complex operational plant. It is not, however, economically attractive to extract uranium from low-grade ores by chemical leaching. Because the content of U3O8 in the Indian ore is usually below 0.1% by weight, it is necessary to develop an alternative process to enable the efficient and economic recovery of uranium. Uranium ore from the Narwapahar mines, UCIL is a type of vein deposit found in India, which accounts for ~54% in India. The ore (0.047% U3O8), though of Singhbhum area, possess exceptionally different mineralogy due to presence of some refractory minerals and richness of apatite (5%) resulting in a maximum 78% recovery through conventional processing at UCIL, with a very high consumption of sulfuric acid and pyrolusite, besides possible loss of uranium as uranium phosphate. To avoid usage of environmentally non-benign oxidants, remediation of influence of phosphates, and improve the overall process output of uranium; an alternate extraction technology using microbial isolate(s) is worthwhile exploring and the same is presented in the paper. The microbial isolate enriched with Acidithiobacillus ferrooxidans grown from source mine water was employed for bio-leaching of uranium while varying pH, pulp density (PD), particle size, etc. On varying pH at 35oC using 20%(w/v) PD and <75µm particle size, 83.5% and 78% uranium bio-recovery were observed at 1.7 and 2.0 pH in 40 days against maximum recovery of 46% and 41% metal in control experiments respectively. Whereas, it was observed that grinding of ore to finer size actually helped dissolution of uranium. Finer size particles (<45µm) gave a maximum biorecovery of 93% in 40days at 10%(w/v) PD, 1.7 pH and 35oC. The bio-recovery of uranium fell down from 93% to 82.73% and 60% on increasing the pulp density from 10% to 20% and 30% respectively for particles of <45µm size. The higher uranium dissolution during bioleaching at 1.7pH can be correlated with increase in redox potential from 433 mV to 664mV in 40 days.

Aug 1, 2010

By H. Sarvamangala

Biomineralization of manganese on titanium condenser material exposed to seawater has been illustrated. Biom-ineralization occurs when the fouling components, namely, the microbes, are able to oxidize minerals present in water and deposit them as insoluble oxides on biofilm surfaces. Extensive biofilm characterization studies showed that an alarmingly large number of bacteria in these biofilms are capable of oxidizing manganese and are, thereby, capable of causing biomineralization on the condenser material exposed to seawater. This paper addresses studies on understanding the exact role of the microbes in bringing about oxidation of manganese. The kinetics of manganese oxidation by marine Gram-positive manganese oxidizing bacterium Bacillus spp. that was isolated from the titanium surface was studied in detail. Manganese oxidation in the presence of Bacil-lus cells, by cell free extract (CFE) and heat-treated cell free extract was also studied. The study confirmed that bacteria mediate manganese oxidation and lead to the formation of biogenic oxides of MnO2, eventually leading to biomineralization on titanium surface exposed to seawater.

Jan 1, 2008

By M. N. Chandraprabha

Arsenopyrite is often associated with sulphides like pyrite, chalcopyrite and galena in refrac-tory gold ores. Selective flotation of arsenopyrite from associated sulphides is desirable for effective gold extraction when the gold is preferentially associated with the arsenopyrite matrix. By contrast, presence of arsenopyrite in flotation tailings poses a serious environmental threat due to arsenic dissolution. To prevent arsenic contamination from the mine wastes, it would be more economical and environmentally beneficial to remove this mineral during the froth flotation step. Use of Acidithiobacillus thiooxidans in the detoxification of arsenic containing sulphides is outlined in this pa-per. Biomodulation using Acidithiobacillus thiooxidans resulted in the preferential depression of pyrite from arsenopyrite. By suitable conditioning with cells and collector effective separation of arsenopyrite from pyrite was achieved. Biomodulation with wild cells did not yield separation of arsenopyrite from chalcopyrite. However, bacterial affinity could be induced to a desired sulfide mineral through environmentally controlled adaptation techniques. Through the use of arsenic-adapted bacterial cells, selective removal of arsenopyrite from chalcopyrite was readily achieved.

Jan 1, 2014

By Sutham Niyomwas

Biomorphic Cellular TiCIC ceramics were produced by vacuum-infiltrating carbon preforms with Ti02 sol and subsequent synthesis by the carbothermal reduction process. The carbon preforms used in the study were obtained by pyrolysis of parawood and neem wood. The effect of infiltration time on the composition and microstructure of the biomorphic TiCIC ceramic was analyzed by X-ray diffractometry and scanning electron microscopy. The biomorphic structure of the TiCIC ceramic is consistent with that of the biotemplate natural parawood and neem wood.

Jan 1, 2008

By R. W. Bartlett

I first met Milton Wadsworth in 1951 as an eighteen year old junior taking his mineral processing course at the University of Utah. He guided my senior thesis, evaluating the then new polymer flocculants on settling uranium leached ore pulps. This was before ion exchange and solvent extraction. After my military service and a brief working stint at Kennecott he supervised my PhD work. He encouraged me to be active in AIME and its constituent societies, where both of us have been directors of AIME and presidents of TMS. He has been a consultant for research groups that I have managed. Above all, he has been my mentor and friend for nearly 45 years. Thus, I am honored and particularly pleased to receive 5MB&apos;s Wadsworth Award.

Jan 1, 1996

By Linlin Tong, Xiangling Song, Guobao Chen, Zhenan Jin, Hongying Yang

Biooxidation of a high arsenic-bearing refractory gold ore was investigated to determine the arsenic removal efficiency by the cultured bacteria and to examine the effect of biological pretreatment on subsequent gold cyanidation. The biooxidation pretreatment process utilizes the ability of adapted acidophilic bacteria to oxidize pyrite and arsenopyrite and release the encapsulated gold. The released gold can be efficiently extracted by the subsequent cyanidation process. The biooxidation experiments were conducted with cultured bacteria using various sizes of agitation tanks. Leaching results showed that over 96.26 % arsenic extraction could be obtained from this ore after 6 days of bioleaching with continuous stirred tank reactor (CSTR). Gold extraction during the cyanidation process was increased from 29.35% to 96.71% after biological pretreatment. The content of arsenic in the biooxidation residue was reduced to below 0.3%. The transformation process of As was considered as [AsS]2- ? As (?) ? As (?) during the biological pretreatment.

Jan 1, 2014

By Richard W. Lawrence

Leaching of copper from sulfide minerals using biooxidation offers a potential route for copper recovery. Oxidation of . sulfide sulfur can proceed either; to sulfate or to elemental sulfur depending on mineralogy and process conditions. The advances in commercial application of biooxidation for sulfidic gold ores has made available operating and cost data which allow the derivation of conceptual costs for copper ore processing for comparison with conventional processing routes. Although costs for copper biooxidation generally remain high relative to conventional smelting, application might be competitive in specific cases. Biooxidation becomes more competitive in the general case if the cost ofS02 emissions in pyrometallurgical processing were to be internalized to a greater degree than currently required. This paper describes the technical aspects of biological processing of copper sulfide ores and concentrates, specifically with respect to the fate of sulfur. The environmental and economic implications of the biooxidation route are discussed in relation to conventional processing.

Jan 1, 1996

By U. Dmitrakova, G. Sedelnikova, D. Kim, E. Savari

"The paper presents the results of research on the development of gold and silver extraction technology from refractory gold sulphide flotation concentrate of one deposits of Nord-East of Russia. The concentrate is due to the complex mineral composition. Gold and silver are finely disseminated in sulphide minerals (arsenopyrite and pyrite partially oxidized by 7-11%). These are characterized by high content of silver 219 g/t and sulphide sulfur 32%, the presence of poorly soluble silver minerals (silver sulfosalts, fahlore, finely dispersed hypergenic acanthite in iron hydroxides and jarosite, etc.), and impurity minerals capable of interacting with cyanide (pyrrhotite, marcasite, nonferrous sulphides) and causing higher than normal cyanide consumption. Pretreatment of refractory sulphide concentrate by biooxidation using a mixed association of mesophilic and moderately thermophilic bacteria at a temperature of 36-40 °C for 120 hours oxidized arsenopyrite by 97.5%, pyrite by 93.5%, unlocked refractory gold, and generally oxidized the minerals absorbing cyanide and oxygen before cyanidation. Further oxidation of these minerals-absorbents was facilitated by the aeration in calcareous medium of the solid biooxidation residues of the concentrate. Sorption cyanidation of the solid biooxidation residues with addition of lead (II) of recovered 91.2% of gold and 82.9% silver from biooxidation residue, compared with 80.1% and 46.6%, respectively, for sorption cyanidation without the preceding biooxidation step. Biooxidation of the concentrate prior to cyanidation improves the recovery of precious metals, especially silver, and significantly reduces cyanide consumption from 18.7 kg/t to 3-5 kg/t of concentrate. INTRODUCTION Biooxidation of gold sulphide concentrates using acidophilic microorganisms has been successfully applied worldwide (van Aswegen et al., 2007; Gericke et al., 2009; Sovmen et al., 2007; Sovmen et al., 2009). At least 15 full-scale plants for tank biooxidation of sulphide concentrates are presently in operation, and the construction of new ones is planned (Bulaev et al, 2015)."

Jan 1, 2018

Bacterial leaching of sulfide minerals are fairly well established in heap, dump and in situ leaching and the primary bacterial treatment of refractory gold concentrate using CSTR have gained industrial prominence in recent years. Three cultures, a mesophile T ferrooxidans (DSM583), a moderate thermophile Sulfobacillus acidophilus (strain YTF1) and a extreme thermophile (Sulfolobus strain BC65) were used for the biooxidation (pre-treatment) of the Porgera gold-bearing pyrite concentrate under optimum O2 and CO2 enriched air (30 per cent O2 and ten per cent CO2) based on volume ratios. The positive effect of using oxygen and carbon dioxide enriched air for the biooxidation of gold-bearing pyrite mineral was clearly demonstrated where an increase in Fe release rate by a factor of 2.8, 1.8 and 1.5 for DSM583, YTF1 and Sulfolobus respectively were observed compared with the shake flask tests. The lag phase observed with the other test conditions such as in shake flask or with air decreased by about 80 per cent for all three cultures. The average observed dissolved oxygen concentrations in the pulp for these tests were 8.7, 5.8 and 5.0 mg/L for DSM583, YTF1 and Sulfolobus respectively, indicating that O2 was not limiting in these tests. The pulp redox potential was observed to increase with time for all tests coincident with the leaching rate and reaching a maximum of 663 mV, however the maximum value differs for each strain, with DSM583 exhibiting the highest followed by YTF1 and Sulfolobus. Biooxidation of pyrite under optimum gas enrichment at different pulp densities showed near total Iron extraction occurred for pulp densities of three, ten and 20 per cent (w/v). At high pulp densities, more so at 40 per cent than 30 per cent (w/v), the leaching rate decreased rapidly for tests with YTF1 and Sulfolobus. Monitoring of dissolved O2 shows that O2 demand increased with increasing pulp density and the average dissolved O2 concentration observed for YTF1 and Sulfolobus at 40 per cent solids concentration was 1.0 mg/L and below, suggesting limiting oxygen and carbon dioxide conditions for these two cultures. The decrease in leaching rate observed at high pulp densities (30 and 40 per cent) under optimum gas enrichment was accompanied by low metal dissolution and low redox potential values which was attributed to shear stress due to attrition of cells by high solids concentration, toxicity of metal ions especially Ag and Fe and the formation of precipitates and jarosites as confirmed from XRD analysis of leach residues hence impeding the leaching process. Limiting conditions due to high pulp density and viscosity were only observed for YTF1 and Sulfolobus. The subsequent cyanide leaching for precious metals from the pre-treated pyrite concentrate in all cases shows the extent of gold and silver recovery increased with the degree of pyrite oxidation and Au and Ag recoveries in excess of 80 per cent were achieved compared with only 18.1 per cent and 35.9 per cent for gold and silver respectively when treated by direct cyanide leaching on the original, untreated concentrate.

Jan 1, 2004

By S. K. Palm

The commercial life of a mineral product can be long, but like hitech electronic and software products that soar in sales one day to become obsolete and forgotten a few months or years, mineral products have a life cycle. They are invented, commercialized and eventually decline and disappear from commerce. Diatomite and perlite are produced from mined minerals, but almost none of the products in these industries spring from the earth in finished form. Straight calcined diatomite was invented over 100 years ago and flux calcined diatomite just a few years later. The expansion properties of perlite were discovered and first exploited about 75 years ago. Some applications for diatomite have disappeared or currently face competition from newer products. Dry cleaning was once the second-largest application for diatomite but disappeared over 30 years ago. Both the beer and wine filtration markets for diatomite face increased competition from membrane filters. A pipeline of new, proprietary products is critical to the future of the industry.

Mar 2, 2022

By Egon Pohl, Peter Eckl, Friedrich Steinhäusler, Johanna Pohl-Rüling

RADON EXPOSURE AND CARCINOGENIC CELL TRANSFORMATION The highest radiation burden to miners is due to inhalation of radon and its short-lived decay products. The quantification of the risk associated with this radiation burden is complicated by modeldependent lung dosimetry, insufficient accuracy of past human exposure data as well as lacking knowledge about potential synergism of various carcinogens. Nevertheless, it is evident from epidemiological studies of miners that inhalation of elevated levels of radon daughters is correlated with increased lung cancer incidence (UNSCEAR, 1977). The situation is further complicated by the lack of reliable data on the biological variability of the latency period for the development of lung tumor under varying exposure conditions. At present information on variation with age is scarce, however, it is indicated that the excess lung cancer rate for workers exposed to high radon levels is considerably higher, if exposure occurs at ages over 40 years as compared to under 30 years of age (Sevc, Kunz, Placek, 1976). Furthermore, latency period is shorter by 6 - 7 years for smokers exposed to elevated radon levels than for non-smokers (Fry, 1976). Analysis of US- and CSR-uranium miner data revealed that the mean latency period is in the order of about 15 years (Jacobi, 1973). These uncertainties, together with medical diagnostic shortcomings, lead to the dissatisfying situation that at present the detection of radon induced injury of mining personal is primarily the assessment of the endpoint, i.e. radiation induced cancerous transformation of lung cells with typically less than 10% chance for recovery. Therefore it would be advantageous to develop practically applicable diagnostic aids to indicate already early precancerogenic changes of cell characteristics. This would permit the identification of specific population groups at increased risk for the development of lung cancer, thereby increasing the chances of more successful medical treatment. Despite continuous improvements in the reliability of test methods for the diagnosis of cancer cells there is still need for techniques, which enable the detection of transformed cells before the onset of invasion of the host organism. It is generally recognized that the transformation of a cell into a malignant state leads to a change of the cell surface, indicating a correlation between membrane-specific immunological properties and loss of growth control. Surface alterations on cells appear to be responsible in part for the malignant state and are not simply secondary effects of the transformation (Schnebli and Burger, 1973). Furthermore, membranes of cancer cells differ significantly from those of normal cells, particularly, in permeability properties (Rastogi, 1976). In dying cells, proteins, glycoproteins, peptides, aminoacids and their breakdown products are released, which block the permeation of the membrane itself. A mammalian cell can be considered as a 3-phase system (interior, boundary, environment) with the membrane forming a heterogeneous barrier between two subsystems. Under normal circumstances a difference of chemical potential is maintained on both sides of the membrane, causing an ion gradient across and fixed surface charges on the membrane. This results in a membrane resting potential (MRP) between cytoplasm and the bulk medium. It has been shown that MRP is a highly sensitive indicator for cellular reactions due to physical, chemical and biological agents, particularly whenever phenomena of transport and ion gradients as well as permeability are involved (Redmann, 1980). The primary interaction of the agent "ionizing radiation" with any form of living matter occurs at the cellular level. The component with the highest hit probability is the cell membrane. In the study presented MRP-changes of human cells were investigated with simultaneous measurements during irradiation and post-irradiation intervals up to six days. Furthermore, the suitability of MRP-measurements of human cells was tested as an indicator for early changes of cellular properties prior to the histological confirmation of carcinogenic transformation. EXPERIMENTAL METHODS Lung cells The biological specimen used were cultured human lung cells and human lung biopsy samples. Monolayers of lung fibroblasts (W138) were grown in petridishes using conventional culture techniques. In addition, transformed lung cells (W138SV13, subline 2RA) were used applying similar culture methods. All petridishes were incubated at 37°C and only cells in logarithmic growth phase were used. Freshly excised lung biopsy samples were obtained from randomly selected male and female patients (20 cases), where suspected or unclear malignancies were indicated by clinical or X-ray observations. At the time of sampling 80 % of the patients were or had been smokers in the past. The samples were taken by means of a stiff bronchoscope introduced into the trachea under local anasthesia with 1 % Novesinsolution (preparation with Atropin and Pantopen). This permitted the observation of both main bronchi down to the segmental bronchials. From each patient two tissue samples were excised from the mucusepithelial layer under visual control with fiber optics and transferred immediately into 1 % NaClsolution. One sample was taken from the suspected

Jan 1, 1981

By Rolf Andreas Lauten, Adam Pratomo, Dani Ramdani, Helminton Sitanggang

A universal challenge for the mineral processing industry is the diminishment of high grade ores. Instead, low grade ores, sometimes also containing problematic gangue minerals is the reality. With lower head grades the industry has to put more effort into processing to maintain the output and achieve suitable recoveries. In an effort to reduce the impact of declining head grade, a biopolymer was evaluated as an additive in the gold leaching operation at Antam Pongkor in Indonesia. Laboratory testing revealed that biopolymer usage lead to an increased gold recovery without apparent negative impact on the other parts of the process. Further plant trials revealed both a recovery increase around 1.3% and higher throughput (9%) in the presence of the biopolymer. Some scenarios that can explain these findings will be discussed.

Jan 1, 2014

By C. Bergstrand

"Precipitation of gypsum and the formation of scale may pose a serious challenge in the leaching of several minerals. As an example, precipitated gypsum may form scale in the machines increasing the need for maintenance. Alternatively, it may encapsulate precious elements, i.e. gold and lower the recovery during subsequent leach processes. In this work we explore how different biopolymers modify the rheology of gypsum at three different pH levels at ambient and elevated temperatures. The ability to disperse gypsum vary with temperature, pH and biopolymer. It is also inversely related to the scaling potential. Measurements of the zeta potential as a function of increasing amounts of biopolymer reveals that the magnitude of the zeta potential changes in the presence of biopolymer, and so does the ability to disperse the gypsum suspension. Similar results with hematite will also be discussed. The methodology and results may aid in the selection of additives used in mineral processing applications where scale pose a problem.INTRODUCTION Processing of refractory gold ores normally involve either atmospheric or oxidation under pressure in order to convert sulfides to oxides (Weir, King, & Robinson, 1986; Weir, Robinson, & King, 1986). This conversion facilitates leaching of gold (and silver) in normal CIL processes. During oxidation, sulfides are converted to sulfates. Normally, there is an abundance of calcium ions in the process water, either released from the ore or added as lime to adjust pH for the CIL process. Precipitation of calcium sulfate, or other compounds (calcium carbonate, jarosite, hematite) is a way to remove gangue minerals from the solution entering the CIL circuit (Ji, King, Fleming, & Ferron, 2010). Precipitation may also cause challenges in the process as gold (or other valuable elements) may become encapsulated in gangue. (Beauchamp, Choung, & Xu, 2006). In addition to precipitation, the precipitated gangue minerals may solidify and form scale in the processing equipment. Recently, Lauten and Kluck (2016) found that dispersants used as scale inhibitors to prevent encapsulation of gypsum did not modify the solubility of gypsum. In the utilised model system, similar amounts of gypsum precipitated with and without dispersants. Instead, it was found that the dispersants evaluated as scale inhibitors reduced the yield stress of gypsum suspensions. The reduced yield stress was associated with a reduced tendency for gypsum particles to agglomerate and form scale."

Jan 1, 2017

By Ross W. Smith

Microorganisms are increasingly finding use in mineral processing and hydrometallurgy both for the enhancement of mineral engineering operations and for the remediation of mineral industry wastes. Some of the applications, such as biologically assisted leaching of sulfide ores and biooxidation of refractory sulfide gold ores, are established commercial processes. Others, such as the use of organisms for the removal of heavy metal ions from dilute aqueous streams, are nearing commercial application. Other uses of microorganisms are potentially possible. These include use of microorganisms in leaching non-sulfide ores, the flocculation or flotation of minerals and remediation of toxic chemicals discharged from mineral engineering operations.

Jan 1, 1993

By Carlos Del Castillo Whittembury

El presente texto señala el uso de la bioRemediación como principal técnica que permite la digestión de residuos orgánicos contaminantes de aguas, suelos y aires. En el caso del agua, su limitación está en el tiempo de mantenimiento (5 días) donde se necesita contar con tanques o pozas acordes con los flujos a tratar, así como el control periódico del sistema. sistema. En el caso de suelos contaminados, es una de las opciones más convenientes por la facilidad de aplicación y la capacidad del tratamiento para remediar. Finalmente, en el uso del aire, los sopladores con membranas porosas mejoran los niveles de oxígeno disuelto, empleando menor energía eléctrica, cuyo aspecto redunda en un menor costo de los tratamientos. En este sentido, se busca mostrar nuevas tendencias de tratamientos a fin de regresar a las condiciones originales las aguas, suelos y aires contaminados por residuos orgánicos, inorgánicos e incluso por hidrocarburos, las cuales están disponibles en el Perú.

Sep 12, 2005

Bioremediation of Acid Mine Water Using Fly Ash and Desulfotomaculum nigrificans One of the major concerns for the mining industry especially after the mine closure is the generation of acid mine drainage (AMD), and its discharge into the environment surrounding the abandoned mines, leading to serious environmental pollution. AMD is usually characterized by high concentration of dissolved metal ions, sulfate and low pH. The efficacy of fly ash was assessed for the removal of metal ions such as iron, zinc and copper as well as sulfate from synthetic acid mine water. Complete removal of ferric and ferrous, 80 per cent of Zn, 83 per cent of Cu and 62 per cent sulfate removal could be achieved, with a concomitant increase in the pH value from 2.3 to 7 using fly ash as an adsorbent under appropriate conditions. The free energy of adsorption of the metal ions onto fly ash was found to be negative, while the adsorption process was endothermic and adhered to the Lagergren pseudo-first order rate equation. Bioremediation studies using a typical sulfate reducing bacterium namely Desulfotomaculum nigrificans, after pre-treatment of the synthetic acid mine water with fly ash, cumulatively resulted in about 94 per cent zinc, 100 per cent copper and 90 per cent sulfate removal. The possible mechanisms of metal ion removal and sulfate reduction from acid mine water utilising Desulfotomaculum nigrificans are discussed.

Sep 13, 2010

By Evvie Chockalingam

Acid mine drainage (AMD) is a perennial problem particularly in abandoned mines, occurring due to the oxidation of sulphidic mine wastes. AMD is usually characterized by high concentration of dissolved metal ions, sulphate and low pH. The efficacies of substrates such as rice husk and the bark of Eucalyptus tereticornis (Sm.) are assessed for the removal of metal ions such as iron, zinc and copper from acid mine water obtained from an abandoned copper mine. Over 99 % of Fe, 98 % of Zn and 95 % of Cu removal could be achieved from acid mine water, with a concomitant increase in the pH value by three units using rice husk as an adsorbent. With respect to tree bark as an adsor-bent, about 96 % of Fe, 75 % of Zn and 92 % of Cu could be removed from the acid mine water with an attendant increase in the pH value by two units. The free energy of adsorption of the metal ions onto rice husk and tree bark is found to be negative in both the cases, while the adsorption process is endothermic in the case of rice husk and exothermic for tree bark. The adsorption process is found to adhere to the Lagergren pseudo-first order rate equation. The reduction of sulphate con-centration in the acid mine water is achieved using a typical sulphate reducing bacteria namely De-sulfotomaculum nigrificans. Desorption experiments indicate that about 88 % of Zn, 87% of Cu and over 77 % of Fe could be recovered from rice husk and over 70 % of Fe, 82 % of Zn and 77 % of Cu could be desorbed from the metal loaded tree bark using 5 M HCl. The possible mechanisms of metal ion and sulphate removal from acid mine water using rice husk and tree bark are discussed.

Jan 1, 2014

By S. Vimalnath

The bioremediation capability of Pseudomonas aeruginosa cells was investigated for the biosorption of lead ions from aqueous solution. The various parameters such as pH, kinetics, biomass loading and concentration of lead affecting the biosorption process were studied. Complete bio removal of lead could be achieved for an initial concentration of 12.4 mg/L at pH 6.2, corresponding to typical industrial effluents. The biosorption isotherm followed Langmuirian behavior and the Gibbs free energy (?G) was determined to be-30.8 kJ/mole, indicative of chemisorption between the functional groups of the bacterial surface and lead. The biosorption kinetics adhered to a pseudo-second order model. The possible mechanisms of interaction of lead with the bacterial cells are discussed. A carbon paste electrode with a coating of biomass was developed for lead ion detection in aqueous solution using cyclic voltammetric technique. Concentrations of lead up to 5x10-5 M could be successfully detected using cyclic voltammetry, which could be improved to a detection limit of 1x10-8 M adopting differential pulse anodic stripping voltammetry (DPASV). Keywords: Pseudomonas aeruginosa, bioremediation, lead, voltammetry

Sep 1, 2012

By S. Carsten, S. Subramanian, R. Vasant Kumar, S. Vimalnath

"The effect of EPS components namely protein, polysaccharide and biosurfactant purified from Pseudomonas fluorescens was investigated for the bioremediation of Pb(II) ions in aqueous solution. Two parameters, namely the amount of EPS component and the concentration of Pb(II) ions were optimized for maximum Pb(II) ions uptake. The EPS components interacted with Pb(II) ions were characterized using energy dispersive X-ray analysis (EDAX) to ascertain the presence of Pb and by attenuated total reflection-Fourier transform infrared spectroscopy (ATR–FTIR) to reveal the functional groups involved in Pb(II) binding. The maximum Pb(II) binding capacities of protein, polysaccharide and biosurfactant were found to be 11.4, 0.55 and 0.11 mg mg-1 respectively. The biosorption processes followed the Langmuir model. The Gibbs free energy (?G0) values of -23.6, -20.9 and -23.2 kJ mol-1 for Pb(II) biosorption by protein, polysaccharide and biosurfactant respectively, attested to spontaneous, chemisorption processes. The ATR–FTIR results revealed the presence and involvement of amino, hydroxyl, carboxyl, phosphate, and methyl functional groups in Pb(II) binding and EDAX analysis confirmed the interaction of Pb with EPS components. The Pb(II) binding mechanisms of EPS have been discussed. The results demonstrate the potential of the purified EPS components of P. fluorescens for removal of Pb(II) ions from aqueous solution INTRODUCTION Waste water containing toxic metals from various industries pose a serious threat to human health and ecosystem. For treating such waste water, conventional methods like chemical precipitation, coagulation, evaporative recovery, electrodialysis, floatation, flocculation, ion exchange, reverse osmosis, nanofiltration, ultrafiltration are used (Lakherwal et al., 2014). These methods have some disadvantages like toxic sludge generation, incomplete metal removal and high cost (Singh and Gadi, 2012), necessitating the search for cheaper and efficient alternative methods. Bioremediation is one of the emerging alternatives where bacteria, fungi, yeast, algae and other biological substances are used as sorbents to remove toxic heavy metals (Vijayaraghavan, et al., 2015). Recently, the bioremediation potential of extracellular polymeric substances (EPS) of microbial origin has attracted attention. However, for Pb(II) removal, there is a paucity of literature focusing on EPS."

Jan 1, 2018