Search Documents

By G. V. Sedelnikova

Biohydrometallurgical studies were performed on the sulfide gold-arsenic concentrate obtained in treatment of refractory ore from a deposit located in northern Russia. Gold is disseminated in sulfides-arsenopyrite, pyrite and antimonite (their content is 10.0%, 30.4% and 0.7%, respectively). Gold extraction is only 11.8% by cyanidation. Results are given for test bacterial oxidation of the concentrate in continuous mode at temp. = 28~32°C using mesophilic bacteria and at temp. = 32~38°C using the association including moderate thermophilic bacteria. The paper shows that the use of moderate thermophilic bacteria allows to speed up sulfide biooxidation and reduce its time to 96 hours compared to leaching at temp. = 28~32°C for 110~120 hours. The optimal range of pH = 1.5~2.0 of biooxidation process was determined where bacterial solutions are produced with the molar ratio Fe:As > 3 necessary for precipitation of hard-to-solve, environmentally safe arsenical precipitates. Electron microscopy showed that sulfide biooxidation results in liberation of disseminated gold with nanoparticle-level sizes. High sorptive activity of carbonaceous matter and other compounds of bacterial residues cause low gold extraction into cyanide solution ? 28.7%. 10% resin load makes it possible to extract 88.1% Au. Aeration of pulp in calcareous medium enhances extraction to 95.7% and decreases cyanide consumption from 17kg/t to 8 kg/t.

Jan 1, 2014

By Tamara F. Kondrateva, Evgenia E. Savari, Galina V. Sedelnikova, Rauf Y. Aslanukov, RAS (INMI), Grigori I. Karavaiko

"The investigation is dedicated to study of biohydrometallurgical processing of refractory gold-arsenic concentrate with the large content of pyrrhotite. Sulfide concentrate of chemical constituents: 75g/t Au, 4,7g/t Ag, 6,3% As, 29,3% S, 2,3% Sb, 0,5% ? (organic) and of mineral composition, %: 11,8 arsenopyrite, 39 pyrrhotite, 12 pyrite, 2,9 antimonite and the rest – aluminosilicates, carbonates and quartz, was studied. The recovery of gold from concentrate by cyanidation is 51,2%. The rest of gold is embedded as finegraded segregation in sulfides.Sulfide oxidation process study is described. Bacterial oxidation was executed on a pilot laboratory unit using combined autotrophic bacterium: A. thiooxidans , A. ferrooxidans, Leptospirillum ferrooxidans and sulfobacillus thermosulfidooxidation, pH=1,5-2,2, t=28-32?C, oxidation time 120 hours. Pyrrhotite is the least stable sulfide mineral with electrode potential 0,45V versus arsenopyrite (0,55 V) and pyrite (0,55-0,6 V) and is oxidated primarily. Pyrrhotite oxidation is followed by large amount of elemental sulphur, which has a bad influence on cyanidation.It’s established that the rate of arsenopyrite (for the first 48 hours) from concentrate containing 39% pyrrhotite is 2-3 times as less as the rate of arsenopyrite bio-oxidation from concentrate without pyrrhotite. The arsenopyrite bio-oxidation is accelerated only after the elemental sulfur was oxidized by bacterium A. thiooxidans and elemental sulfur percentage was reduced from 6,8 to 3,9%. The bacterium concentration is increased from 2,5x107 to 2,5x109 cells/ml. Almost full oxidation of arsenopyrite (98%) and pyrrhotite (98- 99%) is finished in 120 hours. We have studied two pretreatment methods for elimination sulfur content influence on cyanidation: aeration in lime and electrochemical treatment. Such an operation enables to have supplemental oxidation of elemental sulfur and other oxygen and cyanide absorbants before cyanidation. After treatment the content of elemental sulfur was decreased from 3,9 to 2,2%, gold recovery from 90 to 96% and cyanide consumption from 16,2 to 7,2 kg/t decreases. The content of toxical rhodonite-ions in liquid phase of pulp and disinfection reagents consumption were decreased as result of elemental sulfur content in bio-oxidated product reduction."

Jan 1, 2003

The status of utilization of copper ore resources, and the application and development of biohydrometallury technology in the world is reviewed. Based on the characterization of Zambia?s copper resources, biohydrometallurgy technology is shown to be a practical approach to extract and utilize the enormous low grade copper resource, such as ore tailings and low grade copper ore in this country. Through the cooperation of CSU, China and MMMD, Zambia, ?CNMC-CSU Demonstration Base of Biohydrometallurgy Technology in Zambia? was launched and several biohydrometallurgy plants are being constructed. This indicates that the biohdyrometallurgy technology would be applied soon in Zambia and in the whole Africa. Biohydrometallurgy technology stands as an efficient alternative to the traditional pyrometallurgy process and it can be the key to unlock mineral resources value. Keywords: biohydrometallurgy, low grade copper ore, Zambia, Central South University

Sep 1, 2012

By C. N. Eze, C. K. Ezeh, O. Nnabuife, N. M. Ugwoju, S. C. Enemuor, V. Okechukwu

In this study, the efficacy of Providencia vermicola KUBT-1 in the leaching and beneficiation of Agbaja iron ore was evaluated under varying conditions of temperature, ore particle size, and pH. Agbaja iron ore is a Nigerian low-grade ore containing about 43%–52% iron and a high amount of gangue materials, including phosphorus (1.4%–2.0%) and sulphur (0.12%). The study was organised in triplicate in shake flasks with 48 samples. One millilitre of washed cells of Providencia vermicola KUBT-1 suspended in normal saline at 0.5 McFarland standard (108 cells ml-1) was introduced into each flask containing 50 g of sterilised ore in 100 ml of bioleaching medium, and the samples incubated at room temperature for eight weeks. The levels of upgraded iron, mobilised sulphur, and phosphorus were determined by atomic absorption and ultraviolet spectrophotometric analyses, respectively. Results showed that a particle size of 0.8 mm was optimal for iron upgrading and sulphur mobilisation while 1.0 mm ore size was optimal for phosphorus mobilisation. Up to 89.60% of the iron was upgraded while phosphorus and sulphur were mobilised, leaving residual values of 0.223% and 0.02%, in the ore, respectively. The organism produced best beneficiation results at a pH of 2.5 and a temperature of 3ºC. Correlation analysis with the Pearson’s correlation model showed a positive correlation between iron upgrading and sulphur-phosphorus removal (r =1.000, and r =0.967), respectively. These results present Providencia vermicola KUBT-1 as a promising candidate for large scale leaching and beneficiation of Agbaja iron ore.

Dec 15, 2025

By E. H. Cho, B. Conner

Three sulfide minerals, chalcopyrite, sphalerite, and pyrite, were leached using a bioleaching mode and an electrobioleaching mode. The former mode was used to leach the minerals with the bacterium A. ferrooxidans in a bioreactor. In the latter mode, the leaching of the minerals was performed in a combination of a bioreactor and an electrochemical cell. In this set-up, the solution was circulated between the bioreactor and the cathode compartment, the two reactors were in series, and the cell was operated intermittently. The mechanism is such that an increase in Fe(II) concentration, which is a nutrient of the bacteria, by electrochemical reduction of Fe(III) would increase the bacterial population and in turn would accelerate the leaching of the mineral. It has been found that the electrobioleaching of chalcopyrite is an improvement over the bioleaching with respect to the fact that leaching conversion is higher and a high level of Fe(II) concentration can be maintained. However, the electrobioleaching of sphalerite does not show any improvement over bioleaching. The electrobioleaching of pyrite is similar to that of chalcopyrite, and this has potential to be used in applications such as coal desulfurization and pretreatment of refractory gold ore in heap leaching where fine gold particles are entrapped inside pyrite particles.

Jan 1, 2006

By G. Semenchenko

Heterotrophic microorganisms Pseudomonas aureofaciens, microscopic fungi Fusarium sp. and their combinations with some chemical substances were used for gold leaching from refractory ore and flotation concentrateof Akbakaj deposit. All biological additives raised the speed of noble metals dissolution. The best results of gold extraction from ore were in the presence of bacteria or their associations with other solvents and from flotation concentrate in the presence of fungus - 30 % of additional amount of gold. The maximum extraction of gold speed value was during the first 8 hours of process. The electronic-microscopic researches of the ore samples showed almost complete decay of pyrite and arsenopyrite under bacteria influence whereas at chemical leaching most of these minerals stayed uncrushed. The presence of microscopic fungi in flotation concentrate during leaching provided full distraction of pyrite, but some crystals of antimony and arsenopyrite were undestroyed. During cyanide leaching of flotation concentrate there were accumulation of antimony and incomplete pyrite decomposition. Keywords: gold bioleaching, bacteria, fungi, complex solution

Sep 1, 2012

Bioleaching Metals from Waste Printed Circuit Boards and the Shapes of Microorganisms

Sep 13, 2010

By B. A. Paponetti

The following report discusses the bioleaching of chalcopyrite of italian origin catalyzed by Thiobacillus ferrooxidans. The developments vcr.ius time of the main .biochemical and chemical reactions involved in the production of soiuble and insoluble species were investigated focusing on the times at which they begin and bow long they last. The precipitates were characterized both in quantity and in quality relating the rate of the process to the precipitates fonnatiom. A material. balance has been made to verify the proposed mechanism.

Jan 1, 1991

By A. E. Torma

The efficiency of Thiobacillus ferrooxidans in the desulfurization of a high-sulfur content New Mexico coal was demonstrated. The optimum condition of process variables were found to be pH = 2.3, particle size = 38 µm, pulp density 14.1% (W/V) and temperature 33.9°C. Larger scale experiments carried out with these optimum conditions resulted in coal desulfurizations above 97%, while in sterile controls this was only about 10%.- The rate of desulfurization was described with an exponential expression with respect to the effects of coal particle size. The effect of pulp density on coal desulfurization was quantified by a Monod type equation. The apparent activation and deactivation energies, the frequency factor and temperature coefficient were derived from the effects of temperature to be ?aE - 11720 cal mol-1 and A = 30.44 mol dm-3s-lin the range of 20 - 35°C; ?Ed = 43930 cal mol-1 and A = 9.82 x 10-39 mol dm-3s-1 in the range of 35 - 45°C; and Q10 = 1.94 in the range of 20 - 30°C. The thermodynamic values [(?G+, ?H+ and ?S+)] of the activated complex were calculated. The generalized second order regression equation relating the degree of biodesulfurization of coal to the leach parameters was shown to adequately predict coal desulfurization within the range of process variables studied.

Jan 1, 1987

By Umbreen Agha, Stephan L. Chryssoulis, A. Margaritis

"The deportment of gold in samples of the biooxidation feed and product from the Harbour Lights mine in Western Australia was determined using optical microscopy and microbeam techniques such as Secondary Ion Mass Spectrometry (SIMS), Time-of-Flight Laser Ionization Mass Spectrometry (TOF-LIMS), and Electron Probe Microanalysis (EPMA). The main objective was to determine the mechanisms by which acidophilic bacteria (Thiobacillus ferrooxidans) liberate refractory gold which is locked in a sulphide minerals host as visible gold inclusions (> 0.1 µm) and as submicroscopic or 'invisible' gold.Using the integrated mineralogical approach approximately 99% and 100% of the assayed gold in the biooxidation feed and biooxidation product was accounted for. The results were compared to and verified by chemical assays.The gold deportment study indicates that during biooxidation, locked visible and 'invisible' gold is rendered cyanidable by the direct action of Thiobacillus ferrooxidans as well as indirect chemical leaching. Visible gold which is enclosed in sulphide minerals is liberated or partially exposed and is therefore rendered cyanidable as the host mineral is oxidized and dissolved. 'Invisible' gold either coalesces on the surface of the host sulphide mineral as the particle is dissolved or is released into the liquid phase and immediately adsorbs onto the surface of other ore particles with a net negative charge.The shakeflask suspension indicates that direct bacterial oxidation pits and channels correspond to zones of 'invisible' gold and arsenic (in pyrite) enrichment in the host sulphide mineral."

Jan 1, 1996

By E. Lee

The behavior of As bioleaching using Acidithiobacillus thiooxidans (A. thiooxidans) in mine tailings containing highly concentrated As contents (i.e., As=33877 mg/kg) was systematically evaluated according to the changes in temperature (25?40°C, pulp density=0.5%, pH=1.8), pulp density (0.5?4%, temp=30°C, pH=1.8), and initial pH(1.8?2.2, temp=30°C, pulp density=0.5%). The changes in temperature and pulp density affected the As leaching behavior more than the change in initial pH did. Based on the pH and oxidation-reduction potential (ORP) results, the As leaching was attributed to solution acidification. In particular, As leaching was accelerated with increasing sulfate production in the reacting solution. After bioleaching, surface of tailings examined that many pores which dissolved As were formed by a chemical reaction due to bacteria. The As bioleaching efficiency by A. thiooxidans was enhanced by the oxidation of both the added elemental sulfur and the sulfur compounds in the mine tailings. The As bioleaching efficiency was maximized at 50.5% under conditions of an initial pH of 2.2, reaction temperature of 30°C, and pulp density of 0.5%.

Feb 23, 2014

By D. W. Dew

Billiton Process Research has carried out extensive research over the past four years to develop new process technology using bioleaching for extraction of copper and nickel from their sulphide concentrates. Continuous pilot scale and laboratory batch testwork has been carried out with adapted mesophile bacterial cultures at40°C?45°C, moderate thermophile cultures at 50°C?55°C and thermophile cultures at 65°C?85°C. Pilot scale work has demonstrated the commercial viability of mesophile cultures for bioleaching of secondary copper sulphide and nickel sulphide concentrates. Moderate thermophiles offer benefits in terms of reduced cooling requirements for commercial reactors and, in the case of bioleaching of nickel concentrates, some selectivity over bioleaching of pyrite. Continuous pilot scale testwork has shown that thermophiles achieve efficient bioleaching of primary copper sulphide and nickel sulphide concentrates, giving much higher recoveries than achieved by bioleaching with a mesophile or moderate thermophile culture.

Jan 1, 2000

By W. M. Zeng

A mixed culture of moderately thermophilic microorganisms was enriched from Acid Mine Drainage (AMD) samples collected from several chalcopyrite mines in China,which can leach copper from chalcopyrite effectively. Its tolerance to chalcopyrite was brought up to 80 g/L after being adapted to gradually increased concentrations of chalcopyrite. The effects of several leaching variables on copper recovery in Stirred Tank Reactor had been investigated. The ore contained chalcopyrite (62.2%) and galena (25.6%) as the main sulfide minerals. The results of the tests show that it is possible, operating at 8% of pulp density to attain copper extraction of 75% in 44 days. The leaching rate of chalcopyrite tends to increase with the dissolution of total iron. Microscopic counts of microorganisms in the solution tend to be higher at low pH range. Furthermore, the analyses of leached residues indicate that the passivation of this chalcopyrite is mainly due to the formation of a mass of jarosite and anglesite (about 48.3% and 21.7% in residue respectively), other than elemental sulphur layer. The bacterial community composition was analyzed by using amplified ribosomal DNA restriction analysis (ARDRA). Two moderately thermophilic bacteria species were identified as Leptospi-rillum ferriphilum and Acidithiobacillus caldus, with different proportion in the bio-pulp, 67% and 33%, respectively.

Jan 1, 2014

By A. E. Torma

In recent years the copper industry has gained considerable economic stability. An essential part of this industrial wealth is attributable to the successful application of bio-mediated heap and dump leaching techniques in the recovery of copper from low-grade resources. This study evaluated the leachability of a chalcopyrite conlentrate using naturally occurring mixed culture of predominantly Thiobacillusferrooxidans and Thiobacillus thiooxidans. For the effects-of pulp density, the overall reaction rate constant (k) and the order of reaction (n) have been determined to be 1.93 h-1 and 1.36, respectively. Cyclic voltammetric measurements revealed that intermediate reactions are involved in the chalcopyrite oxidation. Using the Price approach, it was shown that the dissolution of chalcopyrite is solid state diffusion controlled.

Jan 1, 1992

Bioleaching of E-Wastes

Sep 13, 2010

By Y. Lv, H. Ye, D. Du

Electrolytic manganese residue (EMR) is a kind of industrial solid waste with high silicon content that contains harmful metal elements such as chromium (Cr), copper (Cu), lead (Pb), cadmium (Cd), nickel (Ni) and cobalt (Co). Developing a highly effective and environmentally friendly process to treat EMR is important, both for the reutilization of metal resources and for environmental sustainability. In this study, the bioleaching of available silicon from EMR using silicate bacteria is investigated, with flask experiments conducted to optimize the parameters in the bioleaching system. For metal ions, the concentrations of manganese (Mn), Co, Ni and Pb in solution were measured to determine the environmental safety effects before and after bioleaching. The results show that the bioleaching rates of available silicon in the slag were mainly affected by the temperature, pH and pulp density. Under the optimal conditions of temperature of 30 °C, pH of 7.0, pulp density of 5 percent, shake flask rotational speed of approximately 210 rpm, particle size of approximately 0.150 mm and leaching time of 16 d, the concentrations of available silicon in the bioleaching system rose to approximately 110 to 140 mg/L. The metals remaining in the residues were mainly bonded in stable fractions, with concentrations lower in the leachate after bioleaching. This study demonstrates that silicate bacteria can be used to increase the contents of available silicon from EMR, and that bioleaching technology can realize the harmless resource utilization of EMR.

By D. Morin

A general study of gold refractory arsenopyrite-pyrite concentrate bioleaching has been carried out including two continuous bioleach testworks at different sizes, namely one at a laboratory scale with a 100 I unit at a 2 kg/d dry solid flowrate and the other at a pilot scale at a 100 kg/d dry solid flowrate. Both tests show a 80% bioleaching of arsenopyrite in about four days of treatment and with the same kinetic. Pyrrhotite is attacked at the earlier stage of the bioleaching but pyrite remains unaffected. The weak potential of oxidation associated with the decomposition of arsenopyrite and the abundance of arsenite leads to a high grade of elemental sulfur in the bioresidue which consumes the major part of the cyanide during the gold recovery. Furthermore. the arsenite and ferrous produced in solution have to be oxidized to obtain a stable precipitate in the waste products and as outlined in previous works of other authors on this subject the addition of iron to obtain a Fe/As molar ratio at least above 3 is necessary. The agitation equipment used in the pilot unit allowed an optimization study calculation for scaling-up the design and power requirements of an industrial installation. For the pretreatment of a gold refractory As high-graded concentrate the reagent cost has the greatest part in the operating cost of the biooxidation step due to the necessary retreatment of the biosolution. In that conditions the power cost is quite marginal. actually it is significant for the treatment of pure pyrite.

Jan 1, 1991

By A. F. Neil, A. S. Bahaj, P. Watkins, P. M. Hyslop, C. E. Kirby, P. A. B. James

The results of initial work indicate that for 3 of the 5 quarry sands bioleaching reduces the iron oxide content to a commercially acceptable 0.035 wt% Fe20 3, although the time taken to achieve this is 14-22 days against a target leach time of 7 day. Subsequent work has identified strains of Aspergillus niger that are more efficient at producing the array of complexing agents for iron and is yielding excellent and rapid reductions in Fe levels. Potentially, bioleaching offers a more environmentally friendly and energy-efficient process for the removal of Fe from glass-making sands than conventional routes but the production of organic acids by fermentation must be achievable with low-cost substrates such as sugar-beet molasses and lactose permeates for bioleaching to offer a commercially viable alternative to treatment with hot mineral acids. Silica sands usually contain three groups of Fe minerals: iron oxyhydroxides, which comprise crystalline types such as goethite and limonite tending to occur as thin, discontinuous coatings and in cracks and crevices on the surface of the quartz; the assemblage of heavy trace minerals, which include ilmenite, rutile, leucoxene, chromite and haematite, commonly present at levels of 0.1 wt% or less as discrete grains or sometimes cemented to the quartz; and clay minerals cemented in pits on the surface of the quartz and in association with weathered, discrete feldspars. The response of the assemblage of iron minerals to short periods of exposure to hot inorganic acids is broadly related to which group they belong to, those that form coatings being accessible to acid and tending to react rapidly whereas the trace minerals are usually resistant and react very slowly, if at all, and the clays are probably digested. The note reports on a preliminary study of the use of microorganisms to develop an alternative clean technology that will give results comparable to those achievable with hot inorganic mineral acids but with more environmentally acceptable procedures. Various fungi and bacteria produce organic acids when grown in certain media. When these acids come into contact with Fe-stained sand grains the iron is removed as soluble complexes. Several strains of Aspergillus niger have been found to produce organic acids from sugar, predominantly citric, gluconic and oxalic acids, their relative yields depending on cultivation medium and conditions. Sand samples were prepared from five quarries in the UK: Levenseat, Lothian; Redhill, Surrey; King's Lynn, Norfolk; Chelford, Cheshire; and Oakamoor, Staffordshire; ranging in iron oxide content from 0.035 to 0.153 wt% Fe203

Jun 18, 1905

By E. G. Noble

The potential for using heterotrophic microorganisms to solubilize manganese from low-grade domestic ores is being assessed at the Reno Research. center, U.S. Bureau of Mines. More than 90 pct of the manganese has been extracted from a variety of oxide ores in shake-flask tests using molasses as a source of .. nutrients for the microorganisms. It has also been demonstrated that high recoveries of manganese can be obtained from a domestic wad ore using column bioleaching. 'l'he isolation and identification of organisms responsible for manganese extraction is discussed, as are the mechanisms responsible for manganese solubilization.

Jan 1, 1991

By H. B. Zhou

The process of bioleaching marmatite using moderately thermophilic bacteria has been studied in this research by comparing marmatite leaching performance of mesophilic bacteria and moderately thermophilic bacteria and valuating the effect of venting capacity on marmatite leaching performance of moderately thermophilic bacteria and the effect of pulp densities on marmatite leaching performance of moderately thermophilic bacteria. The results show that moderately thermophilic bacteria have more advantages over mesophilic bacteria in bioleaching marmatite. Aeration agitation can improve the transfer of O2 and CO2 in solution and promote the growth of bacteria and therefore, enhance the leaching efficiency. With the increase of pulp density, the total leaching amount of valuable metals increased, however, the extraction efficiency decreased due to many rea-sons, like increasing shear force that results in poorly growth condition for bacteria, etc.

Jan 1, 2014