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By Christopher Mark

This proceedings volume contains papers presented at technology transfer seminars sponsored by the National Institute for Occupational Safety and Health (NIOSH) on New Technology for Ground Control in Retreat Mining. The seminars were conducted at five locations: Uniontown, PA (March 26, 1997), Norton, VA (April 8, 1997), Pikeville, KY (April 10, 1997), Charleston, WV (April 17, 1997), and Evansville, IN (April 22, 1997). The papers presented here describe several new, highly practical technologies developed by the NIOSH Pittsburgh and Spokane Research Centers3 to improve safety during pillar retreat operations. Two central issues are addressed: pillar design and mobile roof supports (MRS's). Proper pillar sizing is essential for safe pillar extraction. The Analysis of Retreat Mining Pillar Stability (ARMPS) program and its large data base of actual mining case histories are presented. LAMODEL, a second computer program, can be used for analysis of multiple-seam and other complex mining situations. Other papers address pillar design to avoid massive pillar collapses and the proper role of coal strength testing. MRS's have greatly improved safety where they are used for pillar line support. We studied the application of MRS's at 20 mines throughout the Eastern United States. Conclusions regarding the most effective section layouts, cut sequences, and support placements are reported. Field and laboratory tests of MRS's are also described.

Jan 1, 1997

By Janet Torma-Krajewski

Research has shown that an ergonomics process that identifies risk factors, devises solutions to reduce musculoskeletal disorders (MSDs), and evaluates the effectiveness of the solutions can lower worker exposure to risk factors and MSDs and improve productivity. A review of the Mine Safety and Health Administration (MSHA) injury/illness database indicated that 46% of illnesses in 2004 were associated with repetitive trauma and 35% of nonfatal lost days involved material handling during 2001? 2004. Even though these statistics show that MSDs significantly contribute to occupational illnesses and injuries in the U.S. mining industry, few mining companies have implemented an ergonomics process. Despite the many unique challenges in the mining environment, three mining companies partnered with the MSD Prevention Team at the National Institute for Occupational Safety and Health?s Pittsburgh Research Laboratory to demonstrate that an ergonomics process could be systematically implemented and effectively integrated with existing safety and health programs. Because these three mining companies were very different in organization, culture, and size, the ergonomics processes had to be modified to meet the needs of each company. A description of how these three companies applied ergonomics and the tools and training used to implement their processes is given. Prior to discussing the case studies, general information on the elements of an ergonomics process is provided.

Feb 1, 2009

There is a saying, ?If it moves, it vibrates.? This is especially true in our modern industrial environments, where workers use powered tools, machinery, vehicles, and heavy equipment. How workers are affected by these elements of the industrial working environment is a concern of health professionals, govern­ments, and scientists around the world. How to adequately assess the effects of human vibration exposure is an equally important issue. The human body comprises a dynamic structure that is living, intelligent, and complex. Consequently, it is not unreasonable to consider that exposing the body to an array of vibration environments would result in outcomes that are not necessarily simple or easily predictable. Sensations caused by vibration may include nausea, annoyance, discomfort or pain, and exhilaration or pleasure. Many factors contribute to the effects of vibration on humans, including the characteristics of the motion and the exposed indi­vidual, the activities of the exposed individual, and various elements of the environment. There have been attempts to summarize the effects of occupational vibration exposures by simply recommending the avoidance of certain frequencies of vibration or by providing a graphical method, i.e., a single curve displaying all human responses to all frequencies. However, such efforts have not withstood the scrutiny of scientific research and analysis. Thus, attendees and participants at this professional gathering now have the rare opportunity to benefit from the technological advances and new scientific research to enhance their understanding of human responses to vibration that suggest more than a simplistic approach. In the tradition of the First American Conference on Human Vibration (1st ACHV) held in Morgantown, WV, June 2006, the 2nd ACHV provides a forum for scientists, engineers, medical doctors, industrial hygienists, and educators to learn and advance research and education in the specialized area of human body vibration. The conference was organized by the Mechanical and Industrial Engineering Department, University of Illinois at Chicago, with support from the National Institute for Occupational Safety and Health (NIOSH), Pittsburgh Research Laboratory. As with the first conference, the 2nd ACHV provides the unique opportunity to learn about the current activities of peers and colleagues and to exchange information on topics of interest such as human response, human modeling, experimental design, sensors, new technologies, and epidemiological studies of human responses to hand-transmitted and whole-body vibration. The conference organizers hope that the publication of these conference proceedings continues to foster new research and technological advances to reduce health hazards associated with occupational vibration exposures.

Jun 1, 2009

By A. W. Dougall, T. M. Mmola

"SynopsisThe global resources and commodities market has become highly competitive. While southern Africa’s abundance of minerals resources is still unrivalled, it has lost considerable dominance in terms of production output. The sustainability of southern Africa’s mining industry is increasingly becoming dependent on its ability to manage the performance of its operations well. A valuable tool for monitoring and managing performance is the use of key performance areas (KPAs) – which are those areas of performance that are reflected explicitly or implicitly in the vision and strategies of an organization and reflect the critical success factors of the organization. This paper is a review of key performance areas in the southern African mining delivery environment. The KPAs discussed in this paper have been identified by comparing KPAs of the several mining houses engaged in surface mining operations in southern Africa and then extracting those KPAs that are common to most of them. Although the authors support the view that each organization should develop KPAs to specifically fit its needs, the study reveals that five KPAs – safety and health, costs, product quality, fleet management, and delivery should form a default list that covers the key areas that any organization should consider when choosing KPAs. KPIs are those subsets of KPAs that we measure in order to manage KPAs.IntroductionThe global resources and commodities market has become highly competitive. While southern Africa’s abundance of minerals resources is still unrivalled, it has lost considerable dominance in terms of production output. The sustainability of southern Africa’s mining industry is increasingly becoming dependent on its ability to manage the performance of its operations well. A valuable tool for monitoring and managing performance is the use of key performance areas (KPAs), which are ‘those areas of performance that are reflected explicitly or implicitly in the vision and strategies of the organization’ (Barker, 1997). The terms KPA and KPI (key performance indicator) are often used interchangeably – whether correctly or erroneously is debatable. KPIs are ‘quantifiable measurements, agreed to beforehand, that reflect the critical success factors of an organization’ (O’Neill, 2007). Each KPA probably has multiple KPIs associated with it."

Jan 1, 2015

By R. J. Durrheim, Y. Yabe, M. S. D. Manzi, DSeis team, N. Z. Nkosi, S. B. Mngadi, Jr. H. Ogasawara

On 5 August 2014, the Orkney M5.5 earthquake, the largest in South African gold mining districts, occurred with an unusual strike-slip mechanism at great depth of 4.78 km below the surface. In a rare case, the ML 5.5 earthquake occurred within the area covered by the legacy 2D and 3D reflection seismic data acquired in the 1990s and 2000s for gold exploration and mine development. In addition, the earthquake ML 5.5 rupture plane was recorded and accurately delineated by the underground in-mine seismic network near the source region. The integration of the legacy 2D reflection seismic data and mine seismicity data allowed us to identify a near-vertical structure, striking northnorthwest-south southeast (NNW-SSE). The ICDP-DSeis team drilled three holes (Hole A, Hole B, and Hole C), and two holes (Hole B and Hole C), intersected the upper edges of the ML 5.5 rupture. These holes recovered metasediments, metabasalts, intrusive rocks including dolerite sills and lamprophyre dykes adjacent to the fault zone, and the fault gouge. Late Prof. Tullis Onstott and geomicrobiologists installed a packer in Hole A and successfully recovered saline water and detected gas (~10 MPa). Slip weakening and rupture propagation are significantly influenced by the existence of fault gouges and the production of wear material between two sliding rock surfaces. Using the fault gouge material recovered from Hole C, we conducted friction experiments under dry and room temperature conditions at high slip velocity of ~100 mm/s and normal stress of 2 MPa. The resulting steady-state frictional strength was ~0.66 over a slip weakening distance of ~9.1 m. The steady-state frictional strength was high, which may be caused by large gouge thickness, and the rate of wear generation. As a result, it is proposed that the Moab Khotsong ML 5.5 seismogenic zone is complex and could be controlled by three main processes: a) the complex structural architecture of the seismogenic zone (e.g., intersection of fault, lamprophyre dykes, and dolerite sills); b) the mechanical process induced by tectonic and/or mining related stresses; and c) the mechanical and chemical processes caused by the water and rock interaction.

Jan 12, 2025

By A. Doroudgar, H. Noferesti

Unstable rock failure or rock bursting is one of the most dangerous problems in deep hard rock mines. This type of failure, that occurs uncontrollably, is associated with a significant release of strain energy from the surrounding rock. In this study, the numerical simulation of unstable rock failure was carried out using the three-dimensional explicit Lagranian finite-volume finite difference method to identify the onset and location of unstable failure events in the model. On this basis, a numerical simulation of mine pillars with width-to-height ratios of 0.5, 2 and 3 was performed. A criterion was developed, based on the calculation of the elastic strain energy, to determine the prone areas for the initiation of rockburst in the models. The results of this study showed that, in softer loading systems, due to large amounts of stored elastic strain energy, an unstable equilibrium is established, which leads to the rapid transfer of strain energy to the rock in the form of violent failure. It was also found that the unstable failure starts at the corners and free surface of the model. The new criterion developed in this study may help predict the occurrence of rockbursts in slender pillars and the design of pillars to control human and financial losses caused by this phenomenon.

Nov 27, 2024

By G. E. Agar, W. B. Kipkie, V. K. Drylie

"This paper describes mineral processing test work on drill core samples from an ilmenite deposit in Terrebonne County just north of Montreal.Mineralogical examination showed that the intimate associa¬tion between ilmenite and hematite precluded their separation. The maximum concentrate grade was therefore limited to 22.9% Ti. Liberation of gangue from the hemo-ilmenite grains occurred around 400 µm, a size which proved to be too coarse for recovery by flotation.Other physical separation techniques, such as elec¬trodynamic, magnetic and jigging, were tried in order to take advantage of the relatively coarse liberation size. An all-flotation procedure and a combination of jigging and flotation yielded similar separations.IntroductionInco Metals Company, through its subsidiary Canico, optioned an ilmenite deposit in Terrebonne County, just north of Montreal, and conducted a drilling campaign to confirm previous geological assessments of the deposit and to provide core samples for mineralogical study. Parts of these core samples were used for mineral processing studies, which will be described in this report. The objective was to identify suitable upgrading procedures, to determine the optimum combination of concentrate grade and recovery, and to provide suitable batches of concentrate for further treatment."

Jan 1, 1980

While the United States and the rest of the world struggles with record-setting oil prices Canadian oil sands producers are ramping up production to meet demand for oil while also fighting against a host of negative images. In recent months, the price of oil has skyrocketed. Most of that oil comes from Saudi Arabia. In Alberta, Canada there are unconventional oil reserves that rival those in Saudi Arabia in the vast Canadian oil sands, sometimes called the tar sands. Proponents of the oil sands say that Alberta?s oil sands can be a secure source of oil and energy for the United States for many years to come. With new mining and drilling projects, the industry has said that it could quadruple its production to nearly 4 million bbl/day by 2020. Time magazine once called the oil sands, ?Canada?s greatest buried treasure that could satisfy the world?s demand for petroleum for the next century.? So why hasn?t the United States embraced this close, politically secure source of energy? Because, getting usable fuel from the oil sands can be a dirty business, say opponents. The oil sands are a combination of clay, sand, water and bitumen. It is a heavy, black, thick oil. Oil sands can be mined and processed to extract the oil-rich bitumen, which is then refined into oil. The bitumen cannot be pumped from the ground in its natural state. Instead, tar sand deposits are mined, usually using strip mining or openpit techniques, or the oil is extracted by underground heating with additional upgrading. These methods require a huge amount of energy and water and produce a large amount of carbon emissions. Environmental groups that have long spoken out against the oil sands projects got some political backing in the United States in June when a group of mayors from large U.S. cities spoke out against the oil sands, as did presumptive Democratic candidate for U.S. president Barack Obama?s senior energy advisor. During a convention of large-city mayors in Miami, FL, Alberta?s oil sands were singled out in a nonbinding resolution calling for national guidelines to track the lifecycle impact of different types of fossil fuels. The resolution urges cities not to use unconventional sources of energy with a large carbon footprint, such as liquid coal and oil shale and oil sands.

Jan 1, 2008

Most of the Indian coking coals are medium coking and of very high ash content. Beneficiation of these coals is a must for their utilisation in coke making. During washing while the ash content of the coal is reduced, the same is expected to affect the reactive-inert ratio and the proportion of various petrographic constituents in the clean coal. This will affect the coking behaviour, including the expansion characteristics, of such coal. An increase in expansion behaviour of coal increases the wall pressure and may damage the battery wall. Therefore, close monitoring of coking properties along with drop in ash is necessary for smooth use of the coal in coke ovens. An effort is, therefore, essential to understand the impact of washing on its coking properties. The studies were carried out with captive coals of Tata Steel, India. Three medium coking coal samples namely, VII A, VI A and XI SEB seam coals of West Bokaro Colliery were used for the study. The coal samples after washing to different ash levels were subjected to the following analysis: proximate, crucible swelling number (CSN), LT GK test, dilatometer and plastometer tests and petrography. The experimental data were then analysed to assess the relationship of various coking properties of the coal with its ash content. The studies clearly show that there is a strong impact of beneficiation of the coal on its coking behaviour. The impacts are more at lower level of ash in the clean coal. In general, the coking properties increase with a decrease in ash. This is primarily due to an enrichment of vitrinte materials in the clean coal and an increase in the reactive-inert ratio in the coals. However, the most important impact of the lowering ash is on the increase in the swelling nature of the coal. The coals with lower ash become highly expanding in nature. This may be very critical from operations of coke ovens point of view, especially stamp charged coke ovens. Necessary precaution should be taken in selecting the other coal blend constituents when these coals are beneficiated to a very low level of ash content.

Jan 1, 2014

By O. Rielo, P. Katsabanis

Traditionally fine aluminum is not used in dry commercial explosives due to the hazard associated with aluminum dust explosions during mixing. An industrial process has been developed which enables the safe use of paint grade Aluminum with ANFO. The addition of fine aluminum particles resulted in increased velocity of detonation of ANFO compositions. Velocities of detonation, in 52mm (2 1/16 in.) diameter confined charges were 20% higher than velocities of detonation of straight ANFO. The detonation performance of fine grained ANFO was not benefited by Al at the diameter tested. Furthermore use of larger Al flakes resulted in decreased velocities of detonation. The results are compatible with the experience of Al addition to high explosives. Tests were also conducted using bio-diesel, finding no substantial difference in the performance of AN fuel explosive compositions which used bio-diesel.

Jan 1, 2009

By Jeffrey W. Lin, Larry B. Weston, Jonathan D. Abbott, Jordan L. Arthur

Hydrocarbon fuel oils are one of the main components of ammonium nitrate-based explosives, including ANFO (Ammonium Nitrate-Fuel Oil) and emulsions. While a wide variety of fuels can, and have, been used in the manufacture of ANFO and emulsion products, No. 2 diesel, particularly dyed, off-road diesel, is widely used due to its ready availability and use in fueling mining equipment. However, a number of alternatives to standard diesel have been introduced over recent years, namely, biofuels, ‘high-performance’ diesels, and renewable diesels. The pressure to adopt these alternative fuels come from a variety of sources, such as initiatives to reduce carbon footprints, increased lifetime of equipment, and cost-advantages from tax credits. While these factors may provide a very real benefit to the mine site, a consideration that is often missed is how the use of these fuels can impact the formulation and characteristics of the ammonium nitrate-based explosives. Herein will be presented a discussion of these alternative fuels from a chemical perspective as well as the results of several studies focused on how the inclusion of various alternative diesels influences the production and performance of ammonium nitrate explosives. ANFO and emulsion were manufactured using representative fuel sources and studying in terms of the manufacturing parameters, shelf-life, interaction with other chemicals using in mining explosives. Detonation data was then collected for these products and compared to the behavior of products made with standard diesel fuels.

Jan 21, 2025

By Ebrahim Farrokh

Ghomroud water conveyance tunnel project is under construction using shield Tunnel Boring Machines (TBM) for a total length in excess of 50 km. Phase 1 of the project included 36 km of tunnel divided into four lots (1, 2, 3, and 4) of nine km each. Lots 3 and 4 of this tunnel for a total length of 18 km were combined into one award and have been constructed using a double shield (DS) TBM or one path system. Extreme ground convergence in some sections of the tunnel has stalled TBM performance in early reaches, due to face collapse and shield jammings. This paper presents the latest update on the tunnel progress and impact of ground convergence on machine performance based on the information obtained from field observations. Also, the method and results of tunnel convergence measurements were studied in conjunction with geological parameters and an attempt was made to correlate TBM operational parameters and ground convergence. The result of the analysis indicates a good correlation between machine?s operational parameter and tunnel convergence and can be used as an indicator of the potential for high rates of convergence. An early warning on ground convergence is essential for taking precautionary measures to avoid TBM jamming and related long delays and costs.

By V. Rajaram

The Metropolitan Water Reclamation District of Greater Chicago (MWRD) imposed a no-blasting specification (blasting is restricted to the construction shaft and shaft bottom or boot) on the Deep Tunnel project in 1988. This was prompted by numerous complaints from the residents in the vicinity of the project, and controversies experienced on previous projects from blasting the rock overlying the tunnel. This paper will describe the methods utilized by two major contractors who are driving 20 km (12.5 of tunnel along with drilling over 36 shafts under this restrictive specification. The impacts on design, construction, schedule, cost, and the environment are detailed.

Jan 1, 1991

By Alexandre José Ramos Valentim, Valéria da Silva Guandelini, Fausto Kunioshi

Em um mercado cada vez mais competitivo, garantir serviços com qualidade em ambientes mais produtivos e de custos reduzidos são valores fundamentais para aumentar a eficiência das organizações. Baseada no modelo de gestão de centro de serviços compartilhados, a Diretoria de Suprimentos da Companhia Siderúrgica Nacional criou o BackOffice como uma das formas de dar foco ao core business e tornar estes valores sustentáveis. Este artigo tem como objetivo apresentar a metodologia utilizada para o planejamento, implementação e aprimoramento da área e compartilhar os ganhos de qualidade, performance e custos com a reorganização e padronização de processos que estavam fragmentados e sendo executados em diferentes departamentos.

Oct 1, 2019

By D MacKay

The Mt Wright Project is located near the town of Ravenswood in North Queensland and is operated by Carpentaria Gold – a wholly owned subsidiary of Resolute Mining Limited. Underground Development of the current Mt Wright orebody commenced in June 2006 and the first production ore was brought to surface in June 2007. The orebody is a rhyolite pipe containing fine-grained disseminated gold in a granite host rock. Ground conditions tend to be good to excellent throughout development, with some minor deformation experienced when stope-sized excavations are formed. The mine commenced as a sublevel open stoping (SLOS) operation with the intent to utilise development waste to back fill isolated upper level stopes while developing to the bottom of the known orebody. The mine plan intended to commence production with a bottom-up primary/secondary stoping retreat through the main orebody utilising cemented fill. Some challenges were faced during the initial production phase that included:•Ongoing underground diamond drilling and updating of the block model resulted in some issues reconciling the grade control results to the resource model. This was primarily due to the high variability of grade which in turn caused difficulties in accurately identifying areas of low and high-grade.•The cost of establishing and operating a cemented fill plant to achieve the planned extraction sequence of the orebody had increased significantly beyond initial estimations.•Geotechnical conditions such as stress orientations, ground behavior and response to the forming of excavations learned through the mining of the upper level stopes were different to the assumptions made during the initial design.A number of in-house and external reviews of the mining method were completed which has resulted in a revised mining plan. This paper will summarise the challenges faced and the implementation of the mine plan to date.

Mar 21, 2011

By K. L. Morton

Groundwater and associated pore pressure represent elements that can have a negative impact on surface mining and slope stability. Groundwater flow and deformation within a slope influence each other. An increase in pore pressure will result in a decrease of effective stress; and conversely, if the pore pressure decreases, the effective stress increases. Slope analysis and slope design calculations require good information on pore pressure distribution around an open pit excavation. Until recently very little pore pressure data were available and assumptions had to be used. Location and design of measuring points depend on geological and structural setting, geotechnical domains and practicality. Good planning and the use of sensitive point piezometers to measure in situ pore pressures means that real-time data can be collected and used for the construction of flow nets, the determination of pore pressure distribution around an open pit mine, the validation of the dewatering/depressurization requirements, the calculation of the efficiency of the dewatering system and finally the use of pore pressure data in slope stability analysis. The recommended layout for a monitoring system, types and design of piezometers, data collection and use of the pore pressure data are presented.

Jan 1, 2008

By Scott Turner

PRESIDENT SCOTT TURNER officiated as chairman of the opening session on mining methods, Monday morning, Feb. 15. The first paper was that of Max H. Barber on open-pit mining in the Lake Superior district, read by C. F. Jackson. W. L. Taylor stated that liquid-oxygen explosive had been tried but that the operators are satisfied with the explosives used; lb. of dynamite breaks one ton of rock. Although black powder is considered the most effective explosive, the factor of safety has decided in favor of high explosives. Lucien Eaton told of the Michigan Lime and Chemical Co.'s large L. 0. X. plant at Rogers City, Mich. Mr. Turner referred to this plant, also said that the Bureau of Mines is shortly to issue Bulletin 349 completely covering the use of L. 0. X. from its inception. W. D. B. Motter, Jr., gave some items concerning Chuquicamata, Chile. Twelve years ago the blasts moved 850,000 to 1,000,000 tons, but average shots now move 250,000 to 300,000 tons. One pound of L. 0. X. breaks 3.7 tons, against 3.1 tons by dynamite. Another speaker told of some large dynamite blasts in open pits, one of 360,000 Ib. in March, 1931, and another of 430,000 lb. to be shot in 1932. Tests have been made to determine the effect of large shots on the surrounding country. Mr. Taylor said that 4- and 5-yd. shovels were preferable to those of 8 yd.; their performance is better and cost is lower.

Jan 1, 1932

By Charles Will Wright

THOSE that attended the seventeenth International Geological Congress held in Russia last summer had an excellent opportunity to gather data and to form impressions on the progress made in the industrialization of the country, particularly with regard to the mineral industries. Several trips to places of interest were arranged, supplementary to the technical sessions held in Moscow, the one to the Ural region being that which I selected as being most likely to be of interest. The accomplishments of the last decade in opening up new mines and building new metallurgical plants in the Soviet Union have been outstanding. This could only be accomplished by a central government ready to employ the best technical talent, which was

Jan 1, 1937

By Martin Plante, Denis Hamel, Marie-Pier Bédard, Jeff McLaughlin

"The results and methodology for the optimisation of the zinc flotation circuit at the Matagami Mill using the 6-Sigma methodology are shown in detail while only the base line and final results are presented for the copper circuit. As a result of the six sigma project, the zinc concentrate grade was improved by 2% and the zinc recovery by 3%, with reduced dosage of reagents while the copper concentrate was improved by 2.4%. The process variation in the zinc concentrate grade and recovery was also reduced by over 50%. The main factors contributing to the project success were identified to be improvement in the plant measurement systems, an internal benchmarking exercise and statistically designed laboratory and in plant tests to guide the process improvement. The annual value to Noranda resulting from this project has been estimated at approximately $5 million.INTRODUCTIONThe Matagami concentrator started ore processing from the new Bell- Allard deposit in July 1999. Concentrator performance (grade and recovery of zinc and copper concentrates) was highly variable and work was ongoing to fix the sources of variation. In the mean time, Noranda embarked on its 6-Sigma initiative across the company. The 6-Sigma DMAIC (Define, Measure, Analyse, Improve, Control) methodology was used in the two flotation circuits to reduce variation and improve performance. The statistical 6-Sigma methodology moves towards identifying and controlling critical process operating variables (the X' s) to achieve customerspecified performance (the Y's). The goal of the methodology is to describe a process in a numerical equation known as the transfer function, given as Y = f (XI, X2, X3.. .), where Y could be concentrate grade or recovery and examples of X's are reagent dosage, particle size, etc. With the transfer function, optimal operating conditions and control tolerances are established for the key X's to ensure the desired Y performance is obtained.Each phase of the 6-Sigma methodology is explained below with examples from the Matagami project."

Jan 1, 2001

By J. Kang

To obtain microbial cultures with the ability to efficiently leach copper ion from chalcopyrite, microbial samples from acid mine drainage sites were mixed and then subjected to mutation using sodium nitrite (NaNO2), diethyl sulfate (DES), ultraviolet light (UV) and their combinations. Mixed cultures with a much stronger ability to leach copper ion (identified as mixed cultures A, B, C, D and E) were obtained by screening on leaching media. Leaching tests showed that mixed culture E had the best leaching performance and increased the Cu2+ content by 120.5% in 20 days, compared to a control without mutation treatment. Mixed culture E grows well on Leathen leaching medium supplemented with Cu2+ (at a final concentration of 32 g/L), reaching 3.2 × 107 cells/L after 15 days, while the unmutated control culture cannot survive beyond 15 days, indicating that mixed culture E is more tolerant of high concentrations of Cu2+. In addition, continuous leaching by mixed culture E is very effective at a pulp density of 1%. These results suggest that it has the potential for industrial applications.

Jan 1, 2008