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By R. D. H. Bedd

GEOLOGICAL NOTES The area under discussion is situated approximately 150 m north of Loraine No. 3 shaft at ± 1500 m below surface. The block being mined extends from about halfway between 50 and 52 levels to just below 48 level (see Fig. 1). The EB reefs form the lower portion of the EB zone of the Elsburg series and in the 81 line area the various conglomerate bands show the best development. Up to 18 separate reef bands are developed and the distance from the bottom conglomerate band to the top is ± 26 m. The thickness of the quartzite which forms the middling between the various reef bands varies from 0.1 to 2 m. The strike is roughly north-¬south. The reef bands are payable individually. However, owing to the steepness of the reefs and their close prox¬imity, conventional mining would prove very difficult. The nonpayable reef bands all carry varying amounts of gold and the whole area is a massive payable reef body approximately 26 m wide and 60 m high at 300 to 400 m on strike. The major structure of the area consists of a north¬-south trending syncline. The western flank of the syncline has a steep easterly dip, in places approaching the vertical. This flank cuts out against the overlying rocks of the EA zone. The latter forms a noncon¬formity with the underlying EB zone. The eastern flank of the syncline dips to the west at an average dip of 0.17 rad (10°). The 0.1 rad (6°) north-plunging syncline was sub¬jected to severe faulting. The most important are the Nos. 4 and 10 faults, both of which have a roughly north-south strike. The No. 4 fault dips to the west at an angle of 1.04 rad (60°) with a downthrow of approximately 150 m to the west. The dip of the No. 10 fault is 0.69 to 0.81 rad (40° to 50°) west, with the western block having moved 150 m updip relative to the eastern block. The EB reefs are being mined by the sublevel stoping method down from the sub-outcrop position to the cutoff against the No. 4 fault. On the downthrow side of the No. 10 fault, that is just above the 54 level, two reef bands are payable and will be mined conven¬tionally. ROCK MECHANICS From the section in Fig. 1, it can be seen that the area under consideration is surrounded by stoped-out areas. This will naturally change the stress distribution extensively. The only way to calculate the stress field for such a geometry is to use the finite element method. It was established that this method worked for the present geometry and so the method was applied to predict the stress behavior of the final geometry. From the results, it appeared that the effect of the old stopes was to destress the area. There are, however, some areas-such as the 50 and 48 north footwall haulages-which will require continuous attention. In these two footwall haulages, deterioration can take place due to tensile stress which

Jan 1, 1982

By William P. Walsh

In December 1983 the author undertook an investigation in east Texas which was organized to learn the feasibility of applying geophysical techniques over shallow lignite deposits. The work embraced experimental high resolution seismic reflection and offset Wenner resisitivity sounds over three 4 to 4 mile test sections. This site also was sponsored by Woodward-Clyde Consultants (WCC) and a consortium headed by North American Coal Corporation. The principal objective of this investigation was to learn whether the aforementioned geophysical techniques could be applied over shallow lignite deposits (i.e. located at approximately 100' to 300' in depth) and consequently provide structural and stratigraphic information useful for resource and reserve characterization. A secondary objective was to determine if these same techniques could disclose the nature of the overburden and the distribution of ground water therein.

Jan 1, 1985

By Xiong Tong, Jiushuai Deng, Yingbo Mao, Lingyun Huang

Ammonium carbamate has been used as a reagent for the leaching of copper from the copper oxide ore found in the Honghe state, Yunnan province, China. In order to find the optimal conditions for the leaching of copper from this copper oxide ore,the effects of several processing parameters, including the concentration, time, temperature, particle size and stirring intensity have been investigated. The results of these optimization experiments showed that the leaching rate of copper reached up to 72.76% under the following conditions: liquid-to-solid ratio of 10:1, ammonium carbamate concentration of 2.5mol/L, leaching temperature of 45 °C, stirring speed of 300rpm, a reaction time of 1.5 h and 85% by weight thereof passes through a sieve having 0.074 mm.

Jan 1, 2015

By W. L. Hollinshead, Alwin Poltinger

GENERAL Tunneling by hand digging is as old as the history of humans. Mechanized tunneling did not begin to replace manual methods until around the beginning of the 19th century(Brunel patent, 1818). It brought with it a higher degree of safety for the workers and a much higher rate of boring. But for any kind of tunneling, an accurate and feasible method of survey must be accomplished during mining. That means, that for each tunnel project the most efficient survey method must be devised. The method to be defined depends on the spatial curve, diameter, speed of tunneling method, purpose of tunnel and contractual accuracy. The final accuracy of a finished tunnel is heavily dependent upon three critical elements: 1. Quality of the above ground survey 2. Tunnel traverse and directional transfer from above ground 3. Guidance for the mining job. Combining The Survey And The Guidance System Since the guidance system (GS) is one of the critical factors in the determination of the tunnel final accuracy, it is necessary to analyze how the directional control and tunnel traverse measurements can be effectively integrated into a GS. Any guidance system, whether manual or high technology, must continually detect and compensate for movement of its theodoliteor laser station standpoint. Movement beyond a preset allowable amount must generate a warning or error message to the user. Additionally, optical refraction can interfere with the results. Refraction will have an effect on any kind of optical GS. Therefore, careful selection of target and standpoint locations can minimize, but not completely eliminate, the influence of refraction. Some GS technologies, such as Gyro-based systems, are immune to the effects of refraction, but have other, methodical errors which will be described later in this paper. GUIDANCE Overview Why is a guidance system necessary? A GS, whether manual or automatic, compares the design direction and slope of a tunnel with the current position of the mining

Jan 1, 1997

By D. Mraz

"In certain instances, rocks around underground excavations behave as a plastic mass. This paper offers several solutions to predicting the mode of plastic flow around cavities and designing mining pillars where plastic conditions exist.IntroductionWhen stresses around underground cavities exceed yield limits of the host rock, the material often behaves as a plastic mass. The best example of this phenomenon is the behaviour of salt rocks in deep salt and potash deposits (more than 500 m deep).However, many other soft rocks exhibit similar properties. For example, Jeremic(1) described certain types of coal yielding plastically under high pressure. Alder suggested that granulated rock behaves plastically.The object of this paper is to propose a de sign procedure for mining pillars exhibiting plastic flow due to the combination of rock properties and in-situ stresses.Prandtl(3) mathematically defined a concept of a generalized, perfectly plastic substance, not possessing the property of strain hardening (Fig. I). He had in mind, a solid with a quasi quasi-isotropic , polycrystalline structure having a well defined yield point, and he postulated that its material elements start and continue to deform plastically when the maximum shearing stress ""'MAX"" reaches a sharp limit, dependent on the mean value of the sum of the major and minor principal stresses:"

Jan 1, 1984

By Randall Head

The drilling at the Taum Sauk Reservoir was a unique compilation of the latest technology and everyday common sense. This site is comprised of rhyolite, which is 30 times harder than limestone in comparison. Using some of the most state of the art drills, coupled with GPS guidance, angular drilling systems, and an ordinary piece of string, some of the most amazing drilling feats were accomplished. By constructing the new dam on top of a mountain that had been blasted once before nearly 50 years ago treacherous conditions for the drills and crew were a challenge. The drills had to be flown in by cranes to get to their locations, where they then drilled anywhere from 2.5 to 6 inch (6.4 to 15.2 cm) holes at angles up to 24.4 degrees. Custom drill bits were designed by the drilling crew to tackle feats such as drilling holes up to 120 feet (36.6 m) deep. A main challenge was the shape of the reservoir, as it is not in a straight line, but rather round, so no technological way was readily available to use to drill. Common sense came in and an ordinary piece of string was used to aide in drilling the hole. A revolutionary step was that our drill was used to core our own samples. By using our own equipment, we were able to complete this task for one-third of the cost of having a third party contractor obtain the core samples. On this unmatched jobsite, it has been established that technology has come a long way, but when all else fails, a piece of string will do just fine.

Jan 1, 2010

By K McLeod

{Figure 1} The Placer Dome-Western Areas Joint Venture?s South Deep Twin Shaft Complex, has been planned and dreamt of for nearly 20 years. [ ] The gold reserve at the bottom of these shafts is one of the largest single intact gold deposits remaining in the world today, containing an estimated 57 million ounces. This is about the size of the fictitious reserve created by inventive prospectors for the infamous BREEX scam in Kalimantan about ten years ago and about the size of the reserve Harmony claims as its own in it?s current hostile takeover bid for Gold Fields. So it is certainly of the magnitude that dreams are made of. [ ] {Figure 2} The unique orebody lies in a continuous, highly concentrated, thick deposit, an estimated twelve kilometres along dip and a two kilometre wide strike. Due to the thickness of some sections of the reef, up to 60 metres in places, bulk mining methods will be required for it?s extraction. [ ] {Figure 3} The twin shaft system comprises a main shaft which had an initial design depth of 2765m and a ventilation shaft of 2760m, making them the world?s deepest to be sunk in a single lift. The shafts were

Jan 1, 2005

By Patiño E

Bioleaching is an established technology for the pre-treatment of refractory gold ores and concentrates and the leaching of whole ore copper heaps. In many cases, it offers economic, environmental and technical advantages over pressure oxidation and roasting. However for bioleaching to compete successfully with others pre-treatment processes it needs to be optimized with regard to the parameters that affect the bioleaching reactions and the growth of the microorganism involved. In this sense, this paper focus on the thermodynamic approach applied in bioleaching through of the definition of the growth system and it is thermodynamics analysis, the effect of heat production and energy dissipation on growth and thermodynamic analysis of growth kinetics and the effect of nonstandard conditions.

Sep 12, 2005

By S. P. Sandoval

Previous research by the U.S. Bureau of Mines demonstrated that nonionic surfactants increased the leaching rate of copper in the flask leaching of chalcopyrite with ferric sulfate if sufficient ferric sulfate was present. This research was continued by the Bureau in Part 2 to further explore the application of nonionic surfactants to the dump leaching of chalcopyrite. A polyoxyethylene lauryl ether was selected as the superior nonionic surfactant tested at 25° and 50° C. The addition of the surfactant increased copper leaching by 116 pct at 25° C and 1.0 pct solids and by 61 pct at 50° C and 0.5 pct solids at Fe 3+ concentrations greater than 1 g/L. At 0.1.0 g/L Fe 3+ and below, the addition of the surfactant produced as much as a 35 pct decrease in leaching. Each of the nonionic surfactants studied was found to be heavily adsorbed by a typical chalcopyrite ore, which poses a serious barrier to the application of nonionic surfactants in an actual chalcopyrite dump.

Jan 1, 2010

By Charles Fulton

EXPERIMENTAL work on the process was begun on a laboratory scale at Cleveland, Ohio, in 1914, and transferred to East St. Louis, Ill. in 1916, where a commercial sized furnace was in technical operation until January, 1918. The essential steps of the process are as follows: Oxidized zinc ore or roasted zinc concentrate is mixed with crushed coke and coal-tar pitch and formed into briquets 9.25 in. (23.5 cm.) in diameter and 21 in. (53 cm.) long in a manner similar to that used in the manufacture of graphite or carbon electrodes, except that much less care and time are required. The composition varies with the nature of the ore; in one case, the composition was 100 parts ore, 70 parts coke, and 18 to 20 parts pitch. These briquets maintain their form and volume during and after the distillation of the zinc. This object is gained by using coke as the matrix and coal-tar pitch as the binder; this pitch becomes coke on heating and unites the ore particle and the original coke particle into a continuous mass. The briquet is an electrical conductor but only to such, a degree that it can be used as a resistor. The size of the ore, as in the present practice of zinc metallurgy, ranges from fine material, such as flotation concentrate, to coarse table and fine jig concentrate. The process is not restricted to any particular kind of ore, but is applicable to pure high-grade ores, ores high in iron, and complex zinc lead ores, since the residue from the distillation is held immovably in place within the briquet. In the case of complex zinc-lead ores, the distillation is conducted with a carefully regulated temperature so that a high percentage of lead is retained in the distilled briquet, which may then be smelted for its lead content. In present zinc smelting practice, the reduction fuel is from 40 to 50 per cent. of the weight of the ore. In the briquet, it is from 60 to 85 per cent. of the ore; but unless the ore is exceptionally high in residue, the distilled briquet

Jan 9, 1919

[†AARONSON, ALFRED E., Vice-Pres., Mid-Co. Petroleum Co., Mid-Co. Bldg. Tulsa, Okla. '18 ABADIE, EMILE R., Min. Engr Address wanted. '76 ¦ABADILLA, QUIRICO A. Geol. Dept. Cia. Mexicans de Petroleo "El Aguila," S. A., Edifico "El Aguila," Esq., Calles Aurora y Comercio,] [ ]

Jan 1, 1925

By Ted D. Johnson

INTRODUCTION The tailing dam is part of the SOHIO L-Bar Uranium Mine and Mill located about 70.8 kilometers (44 miles) west of Albuquerque in northwestern New Mexico. The dam location is shown on Figure 1. The tailing dam is one of the last upstream method dams permitted in the industry. Design and construction aspects of the project, as well as operation and maintenance considerations, are reviewed herein. Since initial construction, SOHIO Western Mining Company and Woodward-Clyde Consultants have developed several modifications and improvements in the dam's operating and monitoring procedures. This work has been done in coordination with the New Mexico State Engineer's Office and in accordance with recommendations in a report done for the Corps of Engineer's Phase I Dam Inspection Report. Modifications and improvements described should be of particular interest to owners and operators of other tailing dams utilizing the upstream method of deposition. The tailing facility was designed for a 10-year life to provide an ultimate storage capacity of 6 1/2 billion kilograms (7 1/2 million short tons) in an area of about 180 acres. A plan view of the tailing dam is shown on Figure 2. The tailing consists of approximately 75% sand size material and 25% fines (material passing the No. 200 sieve). The tailing dam includes a small earth starter dam with raises of the dam accomplished using tailing deposited by upstream method techniques. The starter dam is a homogeneous embankment about 610 meters (2000 feet) long, a maximum height of 12 meters (40 feet) and 2 1/2:1 (horizontal to vertical) upstream and downstream slopes. The starter dam contains an internal drainage system which includes a near vertical chimney drain connected to horizontal finger drains. A typical section through the maximum height of the starter dam is shown on Figure 3. A small confining dam, approximately 1.6 meters (5 feet) high, is constructed beyond the limits

Jan 1, 1980

By A. H. Lang

Introduction The recent uranium discovery at Theano point, lake Superior, is probably a rediscovery of the first reported Canadian uranium occurrence. A description of material from the original discovery was published in 1847 by J. L. LeConte, and many attempts have been made to relocate the deposit. Such attempts have in the past been unsuccessful because of vagueness in the original description of the locality. Besides being a prospect that seems to merit development work, the Camray discovery is important because it reaffirms the occurrence of pitchblende in the eastern part of the Precambrian shield. Much staking followed the announcement of the discovery, and some additional finds have been reported. Location The Camray discovery is at the shore of Theano point, lake Superior, about seventy miles north of Sault Ste. Marie (see Figure 1). It is in township 29, range XIV, district of Algoma. The showing is two miles from Highway No. 17, and is reached by a rough trail that leaves the highway at mileage 72.9 from Sault Ste. Marie. There are no towns of any size nearer than Sault Ste. Marie.

Jan 1, 1949

By Emma Smith, R. W. Guard

INVESTIGATION of the nickel-rich end of the Ni-Zr system has been prompted by an interest in the effect of small amounts of zirconium in high temperature alloys. Hansenl presents a hypothetical diagram based on the work of Allibone and Sykes,2 who investigated the system up to 44 atomic pct (55 wt pct) Zr by metallographic means. Since the alloys they used were melted in Alundum crucibles they were probably contaminated with appreciable amounts of aluminum. They found two compounds which they called Ni3Zr and Ni4Zr. Hayes, Roberson, and Paasche3 identified a compound NiZr (50 atomic pct Zr) with a melting point of 1470°C. Such a high melting compound is not compatible with the observation of Allibone and Sykes2 hat a eutectic is formed near 50 atomic pct. The alloys containing up to 60 atomic pct Zr were melted in an inert atmosphere in a noncon-sumable electrode arc furnace. Analysis of most of the alloys indicated negligible loss of zirconium. The alloys were examined in the as-cast condition and also after annealing for 6 hr at 1175°C (or 1000°C for the alloys between 25 atomic pct and 45 atomic pct). Metallographic specimens were etched using mixtures of HF-HNO3-H2O or acetic acid- HN0,-H,O. The specimens for the solidus determinations were wrapped in molybdenum foil and sealed in evacuated quartz tubes. Specimens to be heated above 1300°C were enclosed in zirconia tubes inside the quartz to prevent reaction with molybdenum. The solidus determinations were carried out by heating the specimens at various temperatures for a few minutes and then examining the interior structure metallographically to determine at what temperature melting began. The 150 mesh powders for X-ray analysis were annealed in an evacuated quartz tube for 2 hr at 540 °C to remove cold work. Alloys with 22.5 atomic pct Zr and 44.4 atomic pct Zr required annealing at 700 °C for complete recovery. The grains of both of these alloys were soft and rather ductile. Both Debye-Scherrer film patterns and spectrometer traces were made for each alloy using filtered copper radiation. Results and Discussion The presence of a substantial amount of second phase in an alloy with 1.1 atomic pct Zr indicated that the solid solubility of zirconium in nickel is somewhat less than 1 atomic pct. The a0 value for the face-centered-cubic matrix in the same alloy was equal to that of pure nickel, so that the solubility must be very small. This observation is as expected from the large atom size difference. Annealed specimens of alloys with 15.0, 16.7, 22.5, 28.6, 44.4, and 59.4 atomic pct Zr showed. micro-structures that were essentially single phase. When

Jan 1, 1958

By William E. Ford, Edward Salisbury Dana

The Silicates are m part strictly anhydrous, in part hydrous, as the zeolites and the amorphous clays, etc. Furthermore, a large number of the silicates yield more or less water upon ignition, and in many cases it is known that they are, therefore, to be regarded as basic (or acid) silicates. The line, however, between the strictly anhydrous and hydrous silicates cannot be sharply drawn, since with many species which yield water upon ignition the part played by the elements forming the water is as yet uncertain. Furthermore, in the cases of several groups, the strict arrangement must be deviated from, since the relation of the species is best exhibited by introducing the related hydrous species immediately after the others. This chapter closes with a section including the Titanates, Silico-titanates, Titano-niobates, etc., which connect the Silicates with the Niobates and Tantalates. Some Titanates have already been included among the Oxides.

Jan 1, 1922

By R. V. Hunter, J. F. T. Agapito

Introduction Significant increases in extraction ratio on the order of 6% to 8%, equivalent to as much as 142 t/m (48 st per ft) of panel, have occurred at the Big Island trona mine during the last five years with the help of a practical rock mechanics program. This increase in resource recovery also has contributed to improvements in productivity. Both conventional and continuous machine mining have been used to mine two flat-lying trona beds at depths of 250 m and 260 m (820 ft and 853 ft) in the Green River Formation in southwestern Wyoming. Conventional room-and-pillarmining originally was conducted in panels with extractions of 5 8% to 60%. The use of yield pillars has allowed the panel extraction in increase to 66%. Continuous machine mining was introduced, with a 64% extraction ratio using long, narrow pillars and wide rooms. Further improvements in resource recovery seem feasible in light of present mining experience. The rock mechanics program consisted of field instrumentation to determine the pillar and roof response hole locations. to mining and computer modeling to evaluate the stability of various layouts, which were then adopted for mining. Mining background Stauffer Chemical Co.'s Big Island mine and refinery are located 40 km (25 miles) northwest of Green River, WY. Underground mining operations supply trona, a sodium mineral, to the refinery to produce soda ash. The room-and-pillar method is used to mine two 3 to 3.6 m (10 to 12 ft) thick, flat-laying seams at depths of about 250 to 260 m (820 to 850 ft). Mine production in 1986 was 2.3 Mt (2.5 million st). These were supplied through both conventional and continuous machine mining sec-

Jan 1, 1989

By R. Maurice King

In the operation of today's large scale ore-processing mills, it is extremely important to have some way of making an objective evalu¬ation of the performance of the available flotation reagents. Attempts to do this may often result in inconclusive findings since the magnitude of the difference in the mill results for any two reagents is apt to be quite small compared to the variability introduced by the process and 'the analytical procedures used to measure them. In cases where two reagents give vastly; different mill results, evaluation becomes a simple matter and as such deserves no further attention here. A question of primary concern, however, is how a mill can go about evaluating the performance of two reagents which may, for example, give a difference of only 0.001% copper in the-tailings or of 0.2% in copper recovery. The casual observer may feel that such small differences may be of little importance, but in a mill-whose daily tonnage is between 30 and 40 thousand, these differences could mean a substantial savings. Before the presentation of an actual example of a reagent testing scheme designed for just this-purpose, perhaps a few minutes should be devoted to a review of some statistical methodology to be used later in the discussion.

Jan 1, 1964

By Arthur Knapp

The petroleum industry is confronted with the problem of conservalion. This is not a simple problem in view of a wide difference of opinion among authorities as to whether conservation is necessary and the specific direction that it should take. Many writers accuse the petroleum industry of wasting the natural resources of the country. Others link the consuming public with the industry in this waste. Let us define our terms; agree, if possible, on where and when waste occurs in connection with either the recovery or use of petroleum and select such plans for conservation as appear most practical. A plan for conserv3tion which appears to be logical today may not meet the problem tomorrow. The conservation of petroleum is closely linked with the advancement of marly arts and sciences and as these advance, the plan of conservation has to change. The need for conservation does not necessarily rest upon the elimination of waste. There is not necessarily a fixed connection between waste and conservation. Conservation may be necessary or desirable although there is no waste. Definitions Waste: Useless or unnecessary expenditure. Conservation: The preservation of the natural resources for economical use. Both definitions contain adjectives the interpretation of which is extremely difficult. It is the determination of what expenditures are "unnecessary" or what uses are "economical" that will require study and agreement. If one lets his automobile engine run in cold weather in order to keep it hot, it is difficult to measure that necessity. If an automobile is driven two blocks, when the driver is physically capable of walking, what is the measure of necessity. This question is in the realm of one of the oldest of sociological problems, "What is a luxury?"—a problem which has not been solved. The history of society indicates that the measure of a man's wealth has been the measure of necessity. What he can afford to buy without stinting himself in some other direction, is not a luxury to him and, if it fulfills his desires, may be considered his necessity.

Jan 1, 1927

By G. Cifuentes

It's know that the cathodic purity and the electrolyte treatment is affect for the solid present in the electrolyte. Anodic slimes properties (f.e.: chemical composition, crystalline composition, sedimentation) was studied in copper electrorefining, this properties were modify through by anodic composition and current density. In this paper will be present only the preliminary anodic slimes relationship at different lead anodic composition. The anodic material was doped during the poured process with lead at different composition. It's confirm the apparition of the (Bindheimitte) SMbz07. The specific gravity of this specie is approximately 7.3 - 7.5, with this values is biggest the probability of sedimentation and smaller the slimes not sedimentabel.

Jan 1, 1999

By Eugene V. Cilento, R. Clark Lantz, Kevin A. DiGregorio

"A microscope-based spectrophotometer has been developed to measure superoxide release from single pulmonary alveolar macrophages (PAM) or other phagocytes in culture. Results indicate that PAM respond to stimulation by phorbol myristate acetate (PMA). It appears that some PAM do not release 02 upon stimulation by PMA; however, those that do release 0, show differences in: (I) the total amount of 0, released, (2) the rate of release of 02 and (3) the time delay for release of 02. The significance of measuring 02 release from single cells is two-fold. It allows the simultaneous study of 02 release and electrical potential changes across the membrane of PAM and it allows the study of differences in 02 release among cells. Thus, the function of Normal PAM and the dysfunction of PAM exposed to coal dusts can be more closely studied on single cells.Pulmonary alveolar macrophages (PAM), free cells found in the lungs, protect the lungs by removing inhaled foreign debris and bacteria. The macrophages do this by engulfing and then internalizing the foreign matter (phagocytosis). Stimulation of PAM causes the release of super-oxide radical (02) which has been shown to be the primary metabolite involved in the killing of bacteria by phagocytes (3,8,12). The super-oxide radical is highly reactive and is spontaneously or enzymatically converted to other antibacterial oxidants, including hydrogen peroxide (H202), singlette oxygen ( 02), hydroxyl radical toil'), and hypochlorous acid (HOCl) (2, 3,8,11,12).Exposure of PAM to dusts may lead to dysfunction of this cell-type and to a greater risk of lung damage or infection upon subsequent exposure to toxic dusts. To date, much of the research with this cell-type has used cytotoxicity (cell death) as a measure of dysfunction. Although this is an important end point, PAM dysfunction may occur at doses below those which lead to cell death. In addition, dysfunction has been measured using large numbers of cells. In order to obtain a clear understanding of the mechanisms involved in dysfunction, measurements should be made on single cells. As mentioned earlier, one of the major roles of this cell type is its anti-bacterial actions. Therefore, asessment of release of superoxide radical in single cells is important in determining the functional behavior of PAM before and after exposure to dusts. Since measurements of 02 production have not been made on single cells the purpose of this study is to develop the methodology needed to study release of 02 from single cells.Recent studies have suggested that a relationship exists between the release of 02 and changes in the electrical properties of phagocytes. The change in membrane potential is thought to be the initial step in superoxide production (6,9). Stimulants which cause 02 release also cause changes in the transmembrane potential of phagocytes (9) and in the permeability of the cell membrane to ions (6). For example, stimulants which cause the release of 0, from neutrophils have also been shown to increase the membrane transport of Na+ , K+ and Ca+2 and stimulation of macrophages leads to increases in Na and perhaps Ca permeability with a subsequent change in membrane potential. In some cases, phagocytic activity has been shown to depend upon the extracellular concentrations of these ions (6). Holian and Daniele (7) have shown evidence that the redistribution ofcellular Ca in alveolar macrophages is an initial step leading to 0, release. In order to provide direct evidence that 02- production is related to electrical changes across the plasma membrane of phagocytes, these two phenomena must be analyzed simultaneously on single cells. Ultimately, measurements of 0, release using the methodology developed in this study will be combined with electrophysiological tech¬niques to study both superoxide release and membrane potential changes simultaneiously on single PAM, and to examine the effects of coal and mineral dusts on their physiological fu"

Jan 1, 1988