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By Ray Brainard, Adriana Ventimiglia, Amanda Kerr

The $2 billion Horizon Lateral includes over 30 miles of trenched and tunneled pipeline with diameters up to 120 inches.Related infrastructure includes large and small pumping stations, reservoirs, and flow control facilities. This paper will discuss the strategy and processes used to determine contract packaging and contracting delivery methods for the various project elements with an emphasis on the tunnels and trenchless crossings. Prior Authority experience and the rigorous risk-based approach to develop the preferred alignments played a large role in determining the outcomes along with schedule concerns and permitting and easement acquisition.

Jun 13, 2023

By H. Majima

In spite of the importance of the presence of pyrite in the flotation of sulphide ores, not many studies have been made from the electrochemical point of view. It is generally recognized that the oxidation of sulphide minerals is one of the most Important factors among those which affect the floatability of sulphide minerals. The concept that the presence of oxygen is essential to the flotation of sulphide minerals is supported by numerous experimental results (1). The presence of oxygen in sulphide minerals- aqueous solution systems would affect the electrochemical behaviour of sulphide minerals and subsequently lead to more complicated reactions on the surface of sulphides. Actually some of the phenomena observed in sulphide flotation are deemed to be more appropriately explained from the electrochemical point of view. The author and co-workers have studied extensively the electrode reactions of pyrite and its significance in sulphide flotation. The results have been partly published elsewhere (2-7). In this paper, the author discusses the electrochemical nature of pyrite, and the three different phenomena observed in pyrite flotation: (1) Xanthate¬dixanthogen system on pyrite, (2) Flotation of complex sulphide ores containing pyrite and (3) Depressive mechanisms of pyrite with cyanide. The phenomenon which has been known as an iron contamination effect due to steel grinding media on sulphide flotation is also explained from the electrochemical aspect. However, this problem is not directly concerned with pyrite, and it will be discussed elsewhere (8).

Jan 1, 1971

Peru has become an established mining country. The industry and industry-related businesses account for a significant amount of the country´s revenue, on state and local levels. But like other countries with thriving mining industries, Peru has seen its mining industry revenues fall off in recent years due to a lackluster global economy. And the mining industry in Peru must also deal with increasing concerns about mining?s environmental impacts, local community and other social issues, as well as a regulatory environment that has placed more restrictions on what miners can do. That said, the Peruvian government is beginning to realize how important mining and natural resource development is to building the nation?s economy, improving its infrastructure and lifting its population out of poverty. The government support was evident during the Perumin 2013 mining convention, held in September in Arequipa. In addition to Peru?s President Ollanta Humala speaking at the closing ceremony, the country?s ministers of Energy and Mines, and Economy and Finance participated in the event, as well as numerous other federal and state authorities. Perumin is put on by the Instituto de Ingenieros de Minas del Peru (IIMP, or Peruvian Institute of Mine Engineers). The 31st edition of the convention, billed as the ?world?s largest mining convention? by the IIMP, this event was led by Abraham Chahuan CEO of Antamina and Romulo Mucho (SME member) as president of IIMP, attracted about 118,000 participants from more than 48 countries. This was more than the anticipated 75,000 attendance and well past the 51,000 people who attended the 2011 convention. The last edition of PERUMIN was led by the CEO of Compañía Minera Antamina, Abraham Chahuan and Rómulo Mucho (SME member) and representative of the IIMP. The event attracted more than 1,000 journalists from all over the world, the IIMP said. A total of 2,213 booths from equipment and machinery supplying companies from the five continents were present at the Mining Technology Exhibition (Extemin), including companies from the United States, Canada, China, South Africa, Germany, Australia, Brazil and Chile, among others. The convention has grown so large in recent years, that the IIMP shifted its location from Tecsup vocational school in Arequipa to the Cerro Juli Convention Center. The venue is 116 percent larger than Tecsup, the IIMP reported, covering about 20 ha (40 acres). It was reported by Latinomineria magazine that about 10 million Peruvian soles were invested in the new venue to create a world-class events center. It was also estimated that the event generated more than 234 million soles to Arequipa?s economy.

Feb 1, 2014

By A. Fuerst

This paper delivers insights into how high availability combines with high efficiency at the Yanacocha Plant, Newmont Mining Corporation, Peru. Advances in motor design and maintenance with integration of smart control support the approach plant maintenance staff is applying to deliver such excellent results for availability and efficiency in Yanacocha and elsewhere. Detailed efficiency and availability data for 2011 and 2012 in Yanacocha are provided. ABB and Yanacocha teams collaborate closely by combining their experience and continuous development of gearless mill drive procedures and maintenance knowledge to deliver the impressive performance being achieved. The secrets for high performance shared by the Yanacocha maintenance operations point to their systematic, practical adherence to modern asset management and maintenance concepts.

Feb 27, 2013

By A. W. Khair

This paper presents an analysis of the dynamics of bit wear on the productivity of the continuous miners. Two field surveys have been carried out. The first survey was to assess the impact of bit wear on productivity and production cost. The second survey was to study the wear characteristics of the cutting tool (i.e. magnitude, forms, and mechanisms of bit wear). Primary factors affecting bit wear is identified and laboratory experiments were carried out to study the effects of some of these primary factors on wear in detail. Results indicate that bit wear has a significant effect on the productivity of the continuous miners. Furthermore under the severe geologic conditions (i.e. cutting rock partings and roof and floor rocks) bit wear not only hampers the productivity, but also increases the production cost due to a higher number of bit consumption. This study also indicated that bit wear accelerates beyond 20% of the conical height loss which corresponds to 7% of the weight loss. This increase rate of wear is an indicative of higher abrasion between bit body and cutting material, resulting in a dustier environment.

Jan 1, 1993

By R. D. Williams

Several hydrometallurgical leach processes have been recently proposed for the recovery of copper from copper concentrates. These include the Cymet Process of Cypress Metallurgical Processes Corporation, the Arbiter Process of the Anaconda Company, and the Treadwell Process of Treadwell Engineering company(1). These processes and similar processes for recovery of other minerals, have several phenomenological similarities which make their quantitative description difficult at best. These include (a) the presence of more than one phase, (b) non-uniform mineralization of the feed concentrate, (c) surface and diffusional effects, and (d) a particle size distribution which varies with time. Semi-theoretical, rather than purely empirical, treatment of such processes in their developmental stages will result in a better understanding of the physico-chemical mechanisms in effect. This, in turn, will allow reliable process designs to be made on reasonable time scales for reasonable development costs. Once a production unit is built, such an approach will provide straightforward methods for process control and optimization. A research and development effort to determine criteria and procedures for semi- theoretically characterizing such mineral leach recovery processes has been initiated in a joint venture by the Arizona Bureau of Mines and the College of Mines at the University of Arizona. This group has as its task the mathematical characterization of hydrometallurgical leach recovery processes using semi- theoretical mechanisms for the underlying phenomena. The resulting models are experimentally verified and parameterized on the laboratory scale in such a way that they can be utilized with similarity criteria to provide reliable scaleup to production designs. This discussion will be centered around the elements of such an approach and how they fit together.

Jan 1, 1975

By M. Canty

An innovative biological treatment system designed to detoxify cyanide and nitrate and immobilize metals from cyanide leach operations is described. A pilot-plant demonstration was conducted to detoxify cyanide and immobilize several metals from a process stream at the McCoy/Cove Mine. Testing was performed under the EPA's Mine Waste Technology and Superfund Innovative Technology Evaluation Demonstration Programs. This technology was successful in detoxifying cyanide and effective in removing metals. The pH was consistently neutralized and nitrates were successfully removed. Results show that biological treatment can offer an innovative, cost-effective alternative for the treatment of mining wastewaters.

Jan 1, 1999

By John Carr

The tremendous growth in grinding mill size and horsepower during the '70s demanded advancements in gear cutting technology and equipment for the '80s. Computers are used as an aid in the design and selection of the mill drive system; sophisticated computer graphics are used to create manufacturing drawings; and a new generation of gear cutting machines are used to manufacture the gears. These new gear cutters can generate-cut gears over 40' (12.2 m) in diameter at an AGMA quality level exceeding the currenty acceptable standards. The precision of the resulting gear assures maximum tooth contact, which will result in longer gear set life.

Jan 1, 1981

By Zong‑yu Li, Shao‑jian Ma, Xiao‑jing Yang, Jin‑lin Yang, Xing‑nan Huo, Heng‑jun Li

Optimizing and adjusting the particle size composition of grinding products is of great significance for improving economic benefits and resource recovery and utilization. In this study, grinding contribution and quantitative separation of impact and grinding mechanism in cylindrical mill were creatively researched, and the grinding technical efficiency of impact and grinding was coupled to the grinding technical efficiency of grinding in drop-off mode. The results showed that the contribution rate of impact and grinding in grinding in drop-off mode varied with the grinding time. On the whole, the contribution rate of impact action increases with the grinding time and gradually becomes stable, and the contribution rate of grinding effect was just the opposite. Under the experimental conditions, the main contribution of the grinding process is impact action, and the contribution of grinding action is auxiliary. In addition, the contribution rate of impact and grinding effect in grinding in drop-off mode was also related to the feed particle size. The contribution rate of impact effect decreased with the decrease of feed particle size, while the contribution rate of grinding increased inversely.

Aug 3, 2023

By Jonisha P. Pollard, F. Nasarwanji, Lydia M. Kocher

This study identifies nonfatal injury incidence rates, nature of injury, work activities, glove usage, and sources of hand and finger injuries in the U.S. mining industry, as reported to the Mine Safety and Health Administration (MSHA) from 2011 to 2017. Hand and finger injuries occur at a rate of 6.53 per 1000 full-time employees, which is nearly double the rate of the next highest affected body part, the back.

Feb 24, 2022

By Klaus Rabensteiner, Klaus Chmelina

"INTRODUCTION Urban tunneling is often related with special geotechnical risks. Typical causes are difficult (hydro)geological conditions, a very low overburden and the presence of critical and vulnerable (infra)structures in the influence zone of the construction (e.g. existing tunnels, roads and pavements, sewer pipes, gas and power lines, landmarked buildings, historic monuments). To manage these risks appropriately, an early detection and warning of unexpected behavior of the affected geotechnical objects ground and tunnel, as well as existing structures are mandatory. To accomplish this, the design and execution of a dense geotechnical monitoring program is of high importance. In more detail, this means specifying and performing a systematic and continuous acquisition, processing and analysis of monitoring data to support decision making. In recent years the development of new automatic sensors and (wireless) data transfer techniques and the general trend towards an automatic acquisition of monitoring data has led to an enormous increase of data production frequencies and volumes. In order to handle this data explosion efficiently, information systems and geo-databases have been developed and used successfully in many projects. They represent IT tools that support the different tasks of a given geotechnical monitoring program including data acquisition, transfer, check, processing, backup, analysis, distribution, reporting and, last but not least, alarming. Meanwhile, some tools already have developed the capability to connect to other theme-specific databases used in the project and, thus, serve as a central data management system for all kind of digital data produced. This makes possible the implementation of further useful functions and services supporting tunneling tasks such as machine control, tunnel simulation, geological prediction, process optimization, etc. where data from different engineering disciplines must be combined and processed in an integrated way. In the following chapters our aim is to describe how monitoring data management, geotechnical monitoring, alarming and reporting are organized and technically implemented at the metro project Cityringen in Copenhagen (Denmark). We want to highlight the specific role and tasks of the used tunnel information system in the project, and particularly inform on the most relevant challenges."

Jan 1, 2016

By Alejandro Gutiérrez, David Ahues, Paola Merino, Felipe González

A Discrete Elements Method (DEM) study of load movement within a SAG mill 12,192 × 7620 (40′ × 25′) in normal operating conditions is performed. The purpose is to determine how the geometry of the lifters affects the kinetics of the load inside the mill. Specifically, the changes produced by the incorporation of inclined lifters and spiral or helicoidal pulp lifters to the kinetics are evaluated. Four study cases are identified: first, the base case corresponds to a conventional geometry; the second case corresponds to inclined lifters incorporation; third case corresponds to a mill with helicoidal discharge; and the fourth case corresponds to a mill with inclined lifters and helicoidal pulp lifters. To save computational resources, the water that enters the mill has been modeled by varying the coefficient of restitution. The results of a series of experiments are presented to determine how this coefficient varies when having impacts on surfaces with different liquid levels. A comparative analysis is made of the following variables: mean transport speed and material flow, for normal operating conditions. Taking as reference the mill with straight lifters and radial pulp lifters (case 1), the case with the inclined lifters and radial pulp lifters increases the material discharge flow less to 76 mm of size by 5.1%, and this value increases even more when the helicoidal pulp lifters are incorporated reaching a 58.6%. The most significant change is seen in the flow through the grates and the pulp lifters discharge, which can increase substantially improving the operation.

By Ahmet Unal

Use of radio frequency identification (RFID) technology to solve informational needs in isolated and restricted underground mining environments will be reviewed, giving specifications of some past and currently available systems. The advantages and disadvantages of using various frequencies, system configurations, and specific components, including their construction standards and capability, will be discussed. The remaining challenges to more widespread use of RFID systems for personnel and vehicle tracking will be identified, and recommendations concerning operating environment capabilities will be detailed. Finally, the ultimate requirements for maintaining system integrity during and following a coal mine emergency will be discussed.

Jan 1, 1997

By E. L. Piper

Natural graphite has been known for many centuries, having first been reported as a distinct mineral form by Gessner in 1565. At that time, graphite was used extensively as pencil "lead" because of the ease with which it marks paper. It is a black, unctuous material found as flakes, lumps, or needles in scattered locations throughout the world. The less crystalline, or "amorphous", natural graphites are thought to have been formed from coal under conditions of high temperature and pressure. The more crystalline varieties, such as those mined in Ceylon, probably were precipitated from molten solutions or were pyrolyzed from gaseous carbon compounds. Recognition of the good thermal and electrical conductivity of graphite, together with its highly refractory nature, led to an increasing number of appli¬cations for this material. Clay and pitch bonded natural graphite has been used in crucibles, refractories, foundry facings, and other high-temperature appli¬cations. These forms constitute the most important current uses for natural graphite in the United States. Other products containing natural graphite include batteries, lubricants, pencils, brake linings, etc.

Jan 1, 1973

By Kelly Sands

"Mitigation may be considered a hurdle when it comes to environmental permitting. For complex projects with site specific mitigation plans, the time to devise those plans and get them approved can be a long and costly endeavor. However, mitigation options are expanding and the mitigation banking industry has grown tremendously over the last two decades, helping projects move forward with less debate. Mining companies are recognizing the value that mitigation banks provide in helping to shore up project timelines and costs. Where possible, companies are taking advantage of the opportunities to buy credits from thirdparty providers offering mitigation bank credits. In some instances, mining companies are taking matters into their own hands by establishing mitigation banks to realize their own mitigation freedom. That can have its advantages and disadvantages, as discussed below. All in all, the expansion of mitigation options has streamlined the permitting process, introduced flexibility, improved project permitting timelines and loosened the permitting girdle.Compensatory mitigationCompensatory mitigation involves actions taken to offset unavoidable adverse impacts to wetlands, streams and other aquatic resources and authorized by Clean Water Act section 404 permits and other permits. As such, compensatory mitigation is a critical tool in helping the federal government meet the longstanding national goal of ‘‘no net loss’’ of Waters of the U.S., both in acreage and function.Section 314 of the National Defense Authorization Act for Fiscal Year 2004 required the Secretary of the Army, acting through the Chief of Engineers, to issue regulations “establishing performance standards and criteria for the use, consistent with Section 404 of the Clean Water Act, of on-site, offsite, and in-lieu fee mitigation and mitigation banking.” The provision also required that those regulations ‘‘maximize available credits and opportunities for mitigation, provide flexibility for regional variations in wetland conditions, functions and values, and apply equivalent standards and criteria to each type of compensatory mitigation’’ (73 FR 19595). The result was the Final Rule on Compensatory Mitigation for Losses of Aquatic Resources issued in 2008 (33 CFR Parts 325 and 332).”"

Jan 6, 2017

By W. A. Haley

Wheel loaders became important bulk materials handling tools in the early fifties. As they grew in stature, productivity, reliability, and acceptance, the demand for larger, more rugged machines evolved. In the early sixties, the introduction of a 6-yard model by a major equipment manufacturer led quickly to widespread experimenting and rapid worldwide acceptance in loading blasted rock in stone quarries and smaller metal mines. As the wheel loaders grew in popularity for shot rock loading, larger models, 12- to 15-yard size were developed and saw widespread use by 1970. In the past 20 years, at most quarries and small to medium size mines, most cable operated power shovels have been replaced by large wheel loaders and more recently, hydraulic excavators as the primary loading tool. Wheel loaders larger than the 15-yard class are not used widely. Until about 1970, very few overburden haulage trucks were used in domestic coal mines. About that time, many states started enforcing stringent mine relcamation regulations and in 1977, even more stringent federal legislation was passed. These controls brought about drastic changes in coal surface mining methods in the mountainous areas of Appalachia, in smaller midwestern mines and in the large mines of the western states. Basically, simple material casting methods were replaced at most eastern mines by "haul-back" and "mountain top mining" methods using controlled placement of overburden as it is removed from the coal. These methods dictated much more extensive use of loaders/ shovels and trucks to the point that most coal mining in those areas is now by loader/shovel trucks methods. And those areas have become the home of the country's largest population of large wheel loaders and trucks in the 35- to 85-ton size class with a trend to even larger trucks and shovels. To maintain equal production or provide for growth, the new methods have caused a sharp increase in machine intensity to the extent that at some mines, up to three times as many machines were required to mine a million tons of coal in the late seventies as compared to the machine needs in the sixties. Mining costs and coal prices also took a big jump in the mid-seventies, paralleling the drastic price increases in oil and other forms of energy.

Jan 1, 1984

By Daniel Roy, Danielle Bouffard, Antoine Berton, Simon Gariépy

Commissioning a mineral processing plant has always been a challenging task. The process includes pre- operational verification, dry and wet commissioning, and a gradual ramp-up to full production, all of which must be executed within the shortest possible timeframe, requiring numerous highly interdependent tasks to be conducted concurrently. This phase is under scrutiny by shareholders, corporate management, and other stakeholders who are keen to see a return on investment as the operation starts yielding results. Despite extensive documentation in the literature highlighting its significance, the impact of the commissioning phase remains underestimated in contemporary mining projects. This paper provides an overview of the commissioning process and its associated challenges before focusing on a critical success factor: building a dedicated commissioning team with the required competencies and personality profiles. The roadmap to achieving an efficient plant start-up is outlined. Drawing on over 15 years of experience with more than 20 concentrator commissioning and start-ups, a general framework is presented to maximize success. This method emphasizes the crucial role of the operation and metallurgy team in supporting these projects. Finally, insights from Soutex’s involvement in multiple start-ups are shared, offering lessons learned that emphasize best practices and challenges encountered in expediting the ramp-up process while maintaining high Health, Safety, and Environment (HSE) standards.

Feb 1, 2025

By Edward T. Koch

With the dramatic increase in world demand for basic raw materials, open pit mining is proceeding at an unprecedented rate. A viable demand is the ever tempting Siren's song for increased production or new facilities. When the Siren's lure is viewed against the panorama of today's world economic milieu the concomitant Scylla and Charybdis are seen in the form of high capital cost and high operating cost. If the economic realities of present day open pit mining are to show a proper return on investment, a clear and penetrating eye must be cast upon the area of mine planning. This paper deals with the problem of overburden removal. In almost every open pit mining operation overburden removal is a significant part of the capital cost and operating cost of the total operation. This paper deals with a new and unique method to affect economical overburden removal. Well known to all are the parameters surrounding draglines, shovels, and bucket wheel excavators. All of these machines require a reasonably stable soil to support them and the shovel and bucket wheel excavator require a reasonably stable bank in which to operate. Their reliability, portability and rate of production per dollar of invested capital are good.

Jan 1, 1975

By M. R. Shahan, C. E. Seaman, S. E. Mischler, T. W. Beck

"Float coal dust is produced by various mining methods, carried by ventilating air and deposited on the floor, roof, and ribs of mine airways. If deposited float dust is re-entrained during a methane explosion, without sufficient inert rock dust quantities, this float coal dust can propagate an explosion throughout mining entries. Consequently, controlling float coal dust is of critical interest to mining operations. Rock dusting—adding inert material to airway surfaces—is the main control technique currently utilized by the coal mining industry to reduce the float coal dust explosion hazard. To assist the industry in reducing this hazard, NIOSH’s Pittsburgh Mining Research Division (PMRD) initiated a project to investigate methods and technologies to reduce float coal dust in underground coal mines through prevention, capture, and suppression prior to deposition. Field characterization studies were performed to determine quantitatively the sources, types, and amounts of dust produced during various coal mining processes. The operations chosen for study were a continuous miner section, a longwall section, and a coal handling facility. For each of these operations, the primary dust sources were confirmed to be the continuous mining machine, longwall shearer, and conveyor belt transfer points, respectively. Respirable and total airborne float dust samples were collected and analyzed for each operation and the ratio of total airborne float coal dust to respirable dust was calculated. During the continuous mining process, the ratio of total airborne float coal dust to respirable dust ranged from 10.3 to 13.8. The ratios measured on the longwall face were between 18.5 and 21.5. The total airborne float coal dust to respirable dust ratio observed during belt transport ranged between 7.5 and 21.8. INTRODUCTION During the extraction of coal, various dusts are produced that can be classified into two categories of interest: respirable and float coal dust. Respirable dust (diameter 10.0 = µm) has the capability to penetrate beyond the terminal bronchioles and become deeply embedded into the human lungs, leading towards respiratory disease complications (Potts et al., 1990). Float coal dust is defined as coal dust having a diameter less than 74 µm (Harris et al., 2009). During the mining process, float coal dust can be deposited on the floor, roof, and ribs of mine airways. With the proper mixture of methane gas (between 5% and 15%) and an ignition source, a methane gas explosion can occur (Barker and Humphreys, 1996). The resulting explosion can develop a pressure wave which lifts the deposited float coal dust material into the air, propagating a disastrous coal mine explosion (Harris et al., 2010). The current technique to safeguard against the propagation of a coal mine explosion is to treat the mine airways with incombustible rock dust (typically limestone), maintaining an 80% total incombustible content of the deposited material. NIOSH initiated a project to investigate control technologies that could be aimed at reducing the amount of float coal dust allowed to deposit in the mine airways using three approaches: prevention, capture, and suppression prior to deposition. Reducing the float coal dust being generated during the mining processes and capturing or suppressing airborne float coal dust prior to deposition would in turn increase the efficiency of the applied rock dust used to prevent mine explosions."

Jan 1, 2017

By Egon Pohl, Peter Eckl, Friedrich Steinhäusler, Johanna Pohl-Rüling

RADON EXPOSURE AND CARCINOGENIC CELL TRANSFORMATION The highest radiation burden to miners is due to inhalation of radon and its short-lived decay products. The quantification of the risk associated with this radiation burden is complicated by modeldependent lung dosimetry, insufficient accuracy of past human exposure data as well as lacking knowledge about potential synergism of various carcinogens. Nevertheless, it is evident from epidemiological studies of miners that inhalation of elevated levels of radon daughters is correlated with increased lung cancer incidence (UNSCEAR, 1977). The situation is further complicated by the lack of reliable data on the biological variability of the latency period for the development of lung tumor under varying exposure conditions. At present information on variation with age is scarce, however, it is indicated that the excess lung cancer rate for workers exposed to high radon levels is considerably higher, if exposure occurs at ages over 40 years as compared to under 30 years of age (Sevc, Kunz, Placek, 1976). Furthermore, latency period is shorter by 6 - 7 years for smokers exposed to elevated radon levels than for non-smokers (Fry, 1976). Analysis of US- and CSR-uranium miner data revealed that the mean latency period is in the order of about 15 years (Jacobi, 1973). These uncertainties, together with medical diagnostic shortcomings, lead to the dissatisfying situation that at present the detection of radon induced injury of mining personal is primarily the assessment of the endpoint, i.e. radiation induced cancerous transformation of lung cells with typically less than 10% chance for recovery. Therefore it would be advantageous to develop practically applicable diagnostic aids to indicate already early precancerogenic changes of cell characteristics. This would permit the identification of specific population groups at increased risk for the development of lung cancer, thereby increasing the chances of more successful medical treatment. Despite continuous improvements in the reliability of test methods for the diagnosis of cancer cells there is still need for techniques, which enable the detection of transformed cells before the onset of invasion of the host organism. It is generally recognized that the transformation of a cell into a malignant state leads to a change of the cell surface, indicating a correlation between membrane-specific immunological properties and loss of growth control. Surface alterations on cells appear to be responsible in part for the malignant state and are not simply secondary effects of the transformation (Schnebli and Burger, 1973). Furthermore, membranes of cancer cells differ significantly from those of normal cells, particularly, in permeability properties (Rastogi, 1976). In dying cells, proteins, glycoproteins, peptides, aminoacids and their breakdown products are released, which block the permeation of the membrane itself. A mammalian cell can be considered as a 3-phase system (interior, boundary, environment) with the membrane forming a heterogeneous barrier between two subsystems. Under normal circumstances a difference of chemical potential is maintained on both sides of the membrane, causing an ion gradient across and fixed surface charges on the membrane. This results in a membrane resting potential (MRP) between cytoplasm and the bulk medium. It has been shown that MRP is a highly sensitive indicator for cellular reactions due to physical, chemical and biological agents, particularly whenever phenomena of transport and ion gradients as well as permeability are involved (Redmann, 1980). The primary interaction of the agent "ionizing radiation" with any form of living matter occurs at the cellular level. The component with the highest hit probability is the cell membrane. In the study presented MRP-changes of human cells were investigated with simultaneous measurements during irradiation and post-irradiation intervals up to six days. Furthermore, the suitability of MRP-measurements of human cells was tested as an indicator for early changes of cellular properties prior to the histological confirmation of carcinogenic transformation. EXPERIMENTAL METHODS Lung cells The biological specimen used were cultured human lung cells and human lung biopsy samples. Monolayers of lung fibroblasts (W138) were grown in petridishes using conventional culture techniques. In addition, transformed lung cells (W138SV13, subline 2RA) were used applying similar culture methods. All petridishes were incubated at 37°C and only cells in logarithmic growth phase were used. Freshly excised lung biopsy samples were obtained from randomly selected male and female patients (20 cases), where suspected or unclear malignancies were indicated by clinical or X-ray observations. At the time of sampling 80 % of the patients were or had been smokers in the past. The samples were taken by means of a stiff bronchoscope introduced into the trachea under local anasthesia with 1 % Novesinsolution (preparation with Atropin and Pantopen). This permitted the observation of both main bronchi down to the segmental bronchials. From each patient two tissue samples were excised from the mucusepithelial layer under visual control with fiber optics and transferred immediately into 1 % NaClsolution. One sample was taken from the suspected

Jan 1, 1981