Search Documents

By A. L. de Vegt

For the past ten years Paques has been engaged in the development and installation of treatment systems based on biotechnological processes to remove sulfur compounds from water, air and gaseous streams. Metal and sulfate can be removed from mine waters using two biological steps: 1. Sulfate reducing bacteria convert sulfate to hydrogen sulfide (H2S). The H2S reacts with the dissolved metals to form insoluble metal sulfide precipitates. 2. Sulfide oxidizing bacteria convert excess H2S to elemental sulfur. Paques has designed and installed a groundwater treatment system for the Budelco zinc refinery in the Netherlands to remove metals and sulfate as described above. The system has been operating since May 1992 treating a flow of 5000 m /d. A pilot plant started operation in November 1995 at Kennecott's Bingham Canyon Utah copper mine to develop the processes for metal and sulfate removal from groundwater and selective copper recovery from leach water. The principle of the biotechnological methods, full scale experience, and an overview of the test program at the copper mine are presented.

Jan 1, 1997

By B. Waterman, R. Collins, V. R. K. Paruchuri, P. Gonzalez, H. Dijkman

"INTRODUCTION Mining and processing of sulfide ore bodies has the potential to solubilize sulfate. Gypsum in the host rock, as well as oxidation of sulfides during milling and processing can solubilize sulfate. Acid rock drainage (ARD) also has the potential to generate waters high in sulfate. Even after treatment of the ARD with lime, the treated water can have sulfate present in gypsum saturation levels. Most mine-sites reuse sulfate impacted water but as a mine nears closure, reuse options may be limited and water treatment becomes an option. Furthermore, sulfate discharge requirements, particularly for anti-degredation narrative standards, can be very strict. Freeport has investigated many new technologies to economically remove sulfate from these types of water. A Dutch company, Paques B.V. developed one of these technologies and it utilized biological reactors to convert sulfate into elemental sulfur in a 2-step process. In 1992 the first industrial reference of the SULFATEQTM technology was commissioned at the Nystar Budel zinc refinery in the Netherlands. PILOT PLANT TECHNOLOGY AND DESCRIPTION As shown in Figure 1, the plant contains several process steps, which can mainly be divided into the following sections: 1. Anaerobic biological system in which sulfate is reduced to hydrogen sulfide by sulfate reducing bacteria. 2. Aerobic biological system in which hydrogen sulfide is oxidized to elemental sulfur by sulfide oxidizing bacteria. 3. Solid/liquid separation in which sulfur, calcium carbonate and biomass solids are separated and dewatered. Sulfate impacted ground water is pumped to the feed water tank through a plate and frame heat exchanger to maintain the solution temperature around 95°F. A custom mix of micronutrients is dosed to the feed tank. This nutrient mixture provides essential elements for the growth of anaerobic and aerobic biomass. Urea is added to the feed tank and provides the required nitrogen source to the biomasses. The pretreated feed water is pumped into the anaerobic reactor. The reactor works on the gas lift principle. Sulfate reducing bacteria utilize hydrogen gas as electron donors and reduce sulfate to hydrogen sulfide. This reaction increases the alkalinity of the solution. Carbon dioxide gas is added in order to control the pH within working range of the reactor. Due to the low solubility of the hydrogen gas, the anaerobic gas mixture is recycled through the system. The gas mixture recycle also provides the gas lift for mixing within the anaerobic reactor."

Jan 1, 2016

By Terry I. Mudder

Metal complexed cyanides in wastewaters form as a result of interactions of free cyanide with metals present in the wastewater and exhibit varying degrees of stability, toxicity, and treatability. Thiocyanate, a pollutant commonly found in cyanide bearing wastewaters, is formed through the interaction of free cyanide with sulfur containing species (i. e. pyrrhotite) both present in the wastewater. In certain industrial processes, such as the beneficiation of gold and silver, cyanide is an essential reagent. Since free cyanide, complexed cyanides, and thiocyanate are potentially toxic to humans and aquatic organisms, these compounds must be removed from wastewaters prior to their discharge into surface or ground waters serving as potential potable water sources, marine or fresh water habitats

Jan 1, 1984

By M. Balakrishnan, K. A. Natarajan, J. M. Modak, J. S. Gururaj Naik

The ability of various fungal species isolated from gold mines to uptake gold and base metals such as zinc, copper, iron and lead from gold-process cyanide effluents was investigated. Under identical treatment conditions, different strains showed wide variations in their ability to take up gold and other base metals. Aspergillus terreus exhibited as high as a 60% gold uptake. Almost complete removal of zinc was possible with various fungi and with the yeast Saccharomyces cerevisiae. Studies at various metal concentrations showed a higher gold uptake from dilute solutions, and studies with Aspergillus niger showed that gold uptake by living cells increased gradually as the contact time increased. Metal uptake capacities between living and nonliving cells were also compared. A waste biomass (ABM- I), obtained from a fermentation industry, wasalso identified as having a high gold uptake capacity. At 5% (W/v) solids, ABM-I could efficiently remove up to 83% of the gold contained in an effluent solution within 24 hrs.

Jan 1, 1995

By D. M. Larsen, K. R. Gardner, P. B. Altringer

The Bureau of Mines, U.S. Department of the Interior, is conducting batch and small- scale continuous laboratory studies on the biologically assisted removal of selenium from process waste waters. Mixed cultures of gram- negative bacteria isolated from several brackish water marshes are capable of reducing selenate [Se(VI)I to selenite [Se(IV)I and precipitating an amorphous elemental selenium product. Selenium extractions ranged from 80 to 96% regardless of the initial selenium concentration. The conditions for rapid bacterial growth and reactor design for optimal selenium removal have been investigated. The mechanisms of bacterial reduction and the rate of selenium removal for scaleup to larger systems is discussed.

Jan 1, 1989

By H. Sarvamangala

Biomineralization of manganese on titanium condenser material exposed to seawater has been illustrated. Biom-ineralization occurs when the fouling components, namely, the microbes, are able to oxidize minerals present in water and deposit them as insoluble oxides on biofilm surfaces. Extensive biofilm characterization studies showed that an alarmingly large number of bacteria in these biofilms are capable of oxidizing manganese and are, thereby, capable of causing biomineralization on the condenser material exposed to seawater. This paper addresses studies on understanding the exact role of the microbes in bringing about oxidation of manganese. The kinetics of manganese oxidation by marine Gram-positive manganese oxidizing bacterium Bacillus spp. that was isolated from the titanium surface was studied in detail. Manganese oxidation in the presence of Bacil-lus cells, by cell free extract (CFE) and heat-treated cell free extract was also studied. The study confirmed that bacteria mediate manganese oxidation and lead to the formation of biogenic oxides of MnO2, eventually leading to biomineralization on titanium surface exposed to seawater.

Jan 1, 2008

By G. Whitney, J. Steiner

Introduction The Summitville Mine located in southwestern Colorado is an example of the potential impact that natural weathering and mining activities can have on environmental quality. Acid rock drainage and metals leaching from active and historic mine districts are a major environmental problem facing the mining industry today. Sulfide ore exposed naturally or during mining operations has the potential to produce leachates that contain high concentrations of dissolved metals. These leachates can affect the quality of plant and animal health in surface water used for agriculture, recreation and human consumption. Pintail Systems has developed biological processes for detoxification of cyanide in heap-leached spent ore and process solutions. During application of spent ore cyanide bio-detox processes we have also observed a substantial reduction in many of the leachable metals in biotreatment solutions. These field observations of soluble metal reduction led us to propose that metal bio-mineralization should be a secondary treatment goal of spent ore detox in Focused Feasibility Studies (FFS) for the Summitville Mine. In the FFS (described in a companion paper) for Summitville, spent ore from the heap leach pile (HLP) was loaded into PVC test columns (six inch X ten foot) and was leached with bacteria/nutrient solutions. Two treatment designs were studied: a standard percolation leach (aerobic) and a saturated leach design where the ore was saturated with the heap leach solution to which bacteria and nutrients were applied in a continuous biotreat leach. The bacteria for all column treatment tests were isolated from the spent ore at depth through the Heap Leach Pile (HLP) in the saturated and unsaturated zones. Bacteria isolated from the heap material were tested for cyanide oxidation capacity and were submitted to a bioaugmentation program to improve reaction kinetics and metals tolerance. The final treatment population consisted of several distinct species including aerobic heterotrophs, facultative anaerobes and sulfate-reducing bacteria. The remineralization of soluble metals was observed through a decrease in copper in column leachate solutions and the formation of observable mineral products on ore surfaces in the columns. Several of the tests were run in clear PVC columns to facilitate observation of mineral formation. The column ore contents were photographed before, during and after the biotreatment process which ranged from 10 to 20 days for each test column. Ores were collected from each column after the treatment was complete and were submitted for SEM and TEM study at two laboratories - the USGS SEM Laboratory in Denver, Colorado and the City College of New York SEM laboratory. Treatment Process Description Numerous species of bacteria, fungi and yeasts are capable of accumulating many times their weight in soluble metals. Both living and dead biomass are effective in removing soluble metals from waste streams containing gold, silver, chromium, cadmium, copper, lead, zinc, cobalt and others. Soluble metals may also be immobilized in soils by natural or engineered biomineralization reactions. Several commercial processes using biological reactions are being applied on an industrial scale for metal remediation. Bacteria found in natural and extreme environments have developed a wide variety of metabolic functions to adapt to these environments. These natural microbial functions contribute to global mineral cycling that continuously forms, transforms and degrades minerals and metals in the environment. Biomineralization is described as a surface process associated with microorganism cell walls where the remineralization occurs. The biogeochemical activities initiated by microorganisms in ores, soils, surface and groundwater environments can dominate the formation and transformation of those mineral environments.

Jan 1, 1995

By R. W. Bartlett

I first met Milton Wadsworth in 1951 as an eighteen year old junior taking his mineral processing course at the University of Utah. He guided my senior thesis, evaluating the then new polymer flocculants on settling uranium leached ore pulps. This was before ion exchange and solvent extraction. After my military service and a brief working stint at Kennecott he supervised my PhD work. He encouraged me to be active in AIME and its constituent societies, where both of us have been directors of AIME and presidents of TMS. He has been a consultant for research groups that I have managed. Above all, he has been my mentor and friend for nearly 45 years. Thus, I am honored and particularly pleased to receive 5MB's Wadsworth Award.

Jan 1, 1996

By E. T. Pecina

Bioleaching is a viable option for the processing of auriargentiferous refractory ores with high arsenic content. The application of mesophilic (A. ferrooxidans and ?L. ferrooxidans?) and thermophilic bacteria of the genus Sulfolobus (S. solfataricus and S. acidocaldarius) as oxidant treatment before the cyanidation of an industrial arsenical pyrite concentrate is presented. The results show that extraction of gold and silver increases due to bio-oxidation caused by mesophilic bacteria and results in a maximum gold recovery of 68%. In all strains studied, biooxidation resulted in an average silver recovery of 50%. The results showed that all evaluated strains were sensitive to solids content (5 to 20%) and average particle size (75 and 38 µm) of the concentrate.

Jan 1, 2010

By Jody Kelso, Thomas R. Clark, Gregory J. Olson

Refractory, sulfidic gold ore from the Metates deposit in Mexico was tested to determine if it could be heap biooxidized to improve gold extraction. Small column tests were conducted in a manner consistent with anticipated process conditions: increased tempera­ture due to oxidation of the high sulfide content ofMetates ore and full recycle application of leach solutions containing high concen­trations of dissolved solids. Biooxidation by thermophilic (heat­loving) microorganisms was tested to determine: 1. the extent of sulfide oxidation and bottle roll precious metal recovery, 2. reagent consumption, and 3. potential solution flow problems due to mineral precipitation.

Jan 1, 1998

By John D. Batty

With the emergence ofbiooxidation as a viable process for the pre treatment of refractory gold ores, so have the number of publica­tions on this topic increased dramatically. To a novice in the field of biooxidation, the infonnation presented is often confusing and sometimes even incorrect. Infonnation has also been published that is misleading in that it highlights a particular feature of a process that is either totally irrelevant or of no consequence to the ultimate goal viz. achieving the maximum economical gold recovery. To assist a potential user of biooxidation in evaluating the available published infonnation some guidelines or considera­tions for some of the more important aspects are given, as well as some extracts from publications that demonstrate the manner in which information has been presented.

Jan 1, 1996

By Prisbrey K., N. Choi, A. Burbank

Large tonnages of low grade refractory gold ores are not presently being treated because they resist direct cyanide heap leaching and because they are too low in grade for more expensive oxidizing pre-treatments. If they could be oxidized while remaining in heaps, they could be economically leached with cyanide. The solution is to use bacteria to partially oxidize the encapsulating pyrite mineral which allows cyanide dissolution of the gold in a separate treatment. A variety of refractory ores from different locations was crushed to minus three quarters inch, loaded into columns, and inoculated with T. ferrooxidans. The bacteria attacked the pyrite causing biooxidation, and gold recovery increased 30 to 120 percent after three months curing.

Jan 1, 1990

By S. K. Palm

The commercial life of a mineral product can be long, but like hitech electronic and software products that soar in sales one day to become obsolete and forgotten a few months or years, mineral products have a life cycle. They are invented, commercialized and eventually decline and disappear from commerce. Diatomite and perlite are produced from mined minerals, but almost none of the products in these industries spring from the earth in finished form. Straight calcined diatomite was invented over 100 years ago and flux calcined diatomite just a few years later. The expansion properties of perlite were discovered and first exploited about 75 years ago. Some applications for diatomite have disappeared or currently face competition from newer products. Dry cleaning was once the second-largest application for diatomite but disappeared over 30 years ago. Both the beer and wine filtration markets for diatomite face increased competition from membrane filters. A pipeline of new, proprietary products is critical to the future of the industry.

Mar 2, 2022

By Egon Pohl, Peter Eckl, Friedrich Steinhäusler, Johanna Pohl-Rüling

RADON EXPOSURE AND CARCINOGENIC CELL TRANSFORMATION The highest radiation burden to miners is due to inhalation of radon and its short-lived decay products. The quantification of the risk associated with this radiation burden is complicated by modeldependent lung dosimetry, insufficient accuracy of past human exposure data as well as lacking knowledge about potential synergism of various carcinogens. Nevertheless, it is evident from epidemiological studies of miners that inhalation of elevated levels of radon daughters is correlated with increased lung cancer incidence (UNSCEAR, 1977). The situation is further complicated by the lack of reliable data on the biological variability of the latency period for the development of lung tumor under varying exposure conditions. At present information on variation with age is scarce, however, it is indicated that the excess lung cancer rate for workers exposed to high radon levels is considerably higher, if exposure occurs at ages over 40 years as compared to under 30 years of age (Sevc, Kunz, Placek, 1976). Furthermore, latency period is shorter by 6 - 7 years for smokers exposed to elevated radon levels than for non-smokers (Fry, 1976). Analysis of US- and CSR-uranium miner data revealed that the mean latency period is in the order of about 15 years (Jacobi, 1973). These uncertainties, together with medical diagnostic shortcomings, lead to the dissatisfying situation that at present the detection of radon induced injury of mining personal is primarily the assessment of the endpoint, i.e. radiation induced cancerous transformation of lung cells with typically less than 10% chance for recovery. Therefore it would be advantageous to develop practically applicable diagnostic aids to indicate already early precancerogenic changes of cell characteristics. This would permit the identification of specific population groups at increased risk for the development of lung cancer, thereby increasing the chances of more successful medical treatment. Despite continuous improvements in the reliability of test methods for the diagnosis of cancer cells there is still need for techniques, which enable the detection of transformed cells before the onset of invasion of the host organism. It is generally recognized that the transformation of a cell into a malignant state leads to a change of the cell surface, indicating a correlation between membrane-specific immunological properties and loss of growth control. Surface alterations on cells appear to be responsible in part for the malignant state and are not simply secondary effects of the transformation (Schnebli and Burger, 1973). Furthermore, membranes of cancer cells differ significantly from those of normal cells, particularly, in permeability properties (Rastogi, 1976). In dying cells, proteins, glycoproteins, peptides, aminoacids and their breakdown products are released, which block the permeation of the membrane itself. A mammalian cell can be considered as a 3-phase system (interior, boundary, environment) with the membrane forming a heterogeneous barrier between two subsystems. Under normal circumstances a difference of chemical potential is maintained on both sides of the membrane, causing an ion gradient across and fixed surface charges on the membrane. This results in a membrane resting potential (MRP) between cytoplasm and the bulk medium. It has been shown that MRP is a highly sensitive indicator for cellular reactions due to physical, chemical and biological agents, particularly whenever phenomena of transport and ion gradients as well as permeability are involved (Redmann, 1980). The primary interaction of the agent "ionizing radiation" with any form of living matter occurs at the cellular level. The component with the highest hit probability is the cell membrane. In the study presented MRP-changes of human cells were investigated with simultaneous measurements during irradiation and post-irradiation intervals up to six days. Furthermore, the suitability of MRP-measurements of human cells was tested as an indicator for early changes of cellular properties prior to the histological confirmation of carcinogenic transformation. EXPERIMENTAL METHODS Lung cells The biological specimen used were cultured human lung cells and human lung biopsy samples. Monolayers of lung fibroblasts (W138) were grown in petridishes using conventional culture techniques. In addition, transformed lung cells (W138SV13, subline 2RA) were used applying similar culture methods. All petridishes were incubated at 37°C and only cells in logarithmic growth phase were used. Freshly excised lung biopsy samples were obtained from randomly selected male and female patients (20 cases), where suspected or unclear malignancies were indicated by clinical or X-ray observations. At the time of sampling 80 % of the patients were or had been smokers in the past. The samples were taken by means of a stiff bronchoscope introduced into the trachea under local anasthesia with 1 % Novesinsolution (preparation with Atropin and Pantopen). This permitted the observation of both main bronchi down to the segmental bronchials. From each patient two tissue samples were excised from the mucusepithelial layer under visual control with fiber optics and transferred immediately into 1 % NaClsolution. One sample was taken from the suspected

Jan 1, 1981

By L. Landryova

The Department of Control Systems and Instrumentation, VSB - the Technical University Ostrava, has been involved in a project dealing with utilization of landfill gases, when the methane gas utilization and minimization of its release into the atmosphere was the main concern. The landfill site is generally a place, where technology for energy production can be built. The conditions for this technology implementation vary and depend on various factors. This paper is presenting findings and experience gained regarding a method of methane gas utilization for energy production. This method is concerning building bioreactors at the landfill sites, which are already operated. Every bioreactor can be thought of as a process, which is controlled in order to maximize energy production and minimize the methane gas escape into the atmosphere. The bioreactor landfill can represent a number of environmental and cost savings and, at the same time, is able to comply with all legislative issues.

Jan 1, 2005

By Gh. Crutu

During exploration and exploitation of complex ores, waste dumps that contaminate severely the environment and in particular soils and waters, are genetated. The most hazardous pollutants are heavy metals and metalloids (e.g As, Mo, Pb and Cu) as well as radionuclides (e.g U and Ra).

Jan 1, 2008

By M. Christou

The LIFE-Environment Demonstration Project ?Treating Waste for Restoring Land Sustainability?(www.bangor.ac.uk/ies/TWIRLS/TWIRLS_home.htm), a project initiated by the University of Wales, Bangor, UK in which NAGREF is a partner, aims to demonstrate the effective use of industrial and municipal wastes for the remediation of post-industrial and degraded sites in urban and rural sites. Within the framework of this project, a schist quarry, which is located in a mountainous area near Athens and which has been exploited in the past for cement production, was chosen for bioremediation experiments in collaboration with the quarry owners, TITAN Cement Co. Three different areas within the quarry were selected representing 3 different topographies. These were a compacted, flat area with seasonal water logging (untreated control), a gently sloping, land formed area; and artificially created mounds of overburden rock material formed on the flat area. Compost produced from mixing municipal waste, green waste and digested sewage sludge at the recycling plant of the Association of Communities and Municipalities in the Attica Region (ACMAR), near Athens was used for nutrient amendment. Six replicates of three treatments were applied with varying compost application rates in order to examine the effect of the addition of organic matter in the establishment of suitable vegetation on the infertile schist material. Pine trees (Pinus halepensis Mill.), 4 per replicate, were pocket planted in a volume of 3 liters of schist or schist-compost mixture (1:2 and 2:1). Tree height, main stem diameter and branching were measured following planting and these measurements will be repeated annually to test hypotheses that compaction and water logging reduce Pine establishment by natural regeneration and that nutrient addition improves establishment of pine on schist waste. The availability of nutrients and variation in other soil physico-chemical parameters will also be studied in each treatment and related to plant growth. As a demonstration project, open days are planned on the site for the public and official bodies involved in municipal waste handling and other relevant areas. The aim of these will be to illustrate the effective re-use of waste for the sustainable restoration of post-industrial and degraded land.

Jan 1, 2006

By Janine Acasi, Robert D. Rogers, Jonathan K. Trautman, Richard C. Cronn, James H. Wolfram

The demand for phosphate will continue into the future, because phosphate is currently irreplaceable as a plant nutrient and in many chemical applications. Phosphate is not recycled, so the supply must be replenished through a process of mining and purification. The most energy intensive part of the process, separation of phosphate from the ore, requires 54 to 78% (depending on the method used) of the estimated 0.5 quads of energy consumed by the U.S. phosphate industry. This paper provides data from a project designed to determine if a biotechnology can be developed to separate phosphate from its ore. Several hundred microorganisms (both bacteria and fungi) capable of solubilization of phosphate ore have been isolated from the environment. Work is ongoing to determine the mechanism for solubilization. In addition, how these organisms could be used in a bioprocess is being investigated.

Jan 1, 1989

By C. Xiao, R. Chi

In recent years, the biosolubilization of rock phosphates by phosphate-solubilizing microorganisms has become a major topic of research studies. However, the microorganisms used in previous studies were mostly single and known species, and studies seldom focused on mixed communities of microorganisms or microbial consortia from natural environments. In this study, a microbial consortium from rhizospheric soil was used to solubilize two different compositions of medium- to low-grade rock phosphates — designated as ore samples 1 and 2 — in a laboratory-scale bioreactor. The microbial consortium showed good growth in the bioreactor, reduced the suspension pH and released soluble phosphates from the ores. Scanning electron microscopy with energy-dispersive X-ray spectroscopy showed the action of the microbial consortium on the ore surface. There was considerable corrosion on the ore surface, and 3.42 and 4.93 percent of the element phosphorus was removed from ore samples 1 and 2, respectively. Microorganisms were sampled from the bioreactor for the analysis of their community structures by Illumina MiSeq high-throughput sequencing. The analysis show that the suspensions added with different ore samples had different microbial diversities and richness. This study provides new insight into the biosolubilization of rock phosphates by a microbial consortium directly taken from rhizospheric soils.

By K. A. Natarajan, J. M. Modak, B. Saha

Nonliving waste biomass consisting of Aspergillus niger attached to wheat bran was used as a biosorbent for the removal of copper and zinc from aqueous solutions. Copper and zinc uptake by the biomass obeyed Langmuir isotherms. The binding capacity of the biomass for copper was found to be higher than that for zinc. The metal uptake, expressed in milligrams per grain of biomass, was found to be a function of: •the initial metal concentration (with the uptake decreasing with increasing initial concentration), •the biomass loading (with the uptake decreasing with increasing biomass loading) and •pH (with the uptake increasing with increasing pH in the range of L5 and 6.0). The metal uptake was significantly affected in the presence of a co-ion. The uptake of copper by the biomass decreased in the presence of zinc and vice versa. The decrease in metal uptake was dependent on the concentrations of metals in the two-component aqueous solutions. The effect of copper on zinc uptake was more pronounced than the effect of zinc on copper uptake.

Jan 1, 1997