Technique to Determine Vibration Patterns of Excised Rat Lungs

"To help evaluate the vibration patterns of an excised lung, a method is developed to determine which parts of the surface of an excised lung are responding to acoustic excitation via the trachea. Holographic interferometry, a very' sensitive technique ·that can measure movements of a few microns, is used to detect the lung surface movements.The intact lung and heart are excised from Long· Evans male rats weighing 250 gm. A tube is inserted into the trachea and connected via a funnel to a 3 inch cone speaker. The assembly is positioned in the holographic unit and excited at frequencies up to 10,000 Hz. The holographic unit contains an acousto-optic shutter used with a 0.5 watt Argon laser.The resulting reconstructed holograms contain interference fringes that are indicated of lung surface vibration.INTRODUCTIONA 1986 report by the U.S. National Committee on Biomechanics [1] describes diseases of the lung as a major national health problem. One of the recommendations of the report is for continued study of the mechanical properties of lung tissue. As an example, the report cites at least three areas that would benefit from a better understanding of the vibratory patterns or the lung."