Role of a heterotrophic Paenibacillus polymyxa bacteria in the bioflotation of some sulfide minerals

A pure strain of Paenibacillus polymyxa and mineral- adapted strains are used to bring about surface chemical changes on pyrite and chalcopyrite and, thus, theirflotation. Paenibacillus polymyxa was adapted by repeated subculturing in the presence of pyrite and chalcopyrite. The surface chemical changes of bacteria due to adaptation and of minerals after their interaction with bacterial cultures are evaluated by electrokinetic and infrared spectroscopy and are discussed with reference to their flotation responses. Interaction of bacterial cells, bacterial metabolites and whole bacterial cultures affected the Hallirnondflotation behavior of the sulfide minerals. The Xanthate flotation results show that pyrite, but not chalcopyrite, is depressed when the tests are carried out after interaction with chalcopyrite-adapted Paenibacillus polymyxa. This investigation demonstrated that the surface chemical properties of bacteria can be manipulated successfully to achieve the desired effects in the flotation process.