RI 3282 A Procedure for the Removal and Determination of Small Amounts of Benzene in Biological Material

"In a study of the chemistry and pathology of chronic benzene poisoning, which was conducted cooperatively by the United. States Bureau of Mines, the Barrett Co., and the producers of benzene, it was necessary to make quantitative determinatious of the abzorption, distribution, and: fate of benzene in the organism. Although methods for determining benzene in relatively large amounts were available no satisfactory method was found for removing and determining the small amounts that are present in the tissues or excretions after exposure to conditions which ultimately cause chronic poisoning. This no doubt accounts for the lack of information in the literature on this particular phase of benzene poisoning.The writers have found in work that will be described in a subsequent publication that the amounts of benzene present in the tissues and excretions under conditions of exposure which produce chronic benzene poisoning arc of the order of a fraction to a few milligrams per 100 grams of tissue. This report describes a method developed and found satisfactory for these determina¬tions.RÉSUMÉ OF PERVIOUS METHODSA method for the determination of benzene in tissue was developed by Joachimoglu. 5 In the Joachimoglu procedure the ground organs are mixed with water and acidified with diluted sulphuzic acid, and the benzene is distilled into a receiver containing carbon tetrachloride. The benzene is nitrated in the carbon tetrachloride, the carbon tetrachloride evaporated, the acid mix¬ture neutralized, and the dinitrobenzene extracted with ether. After removal of the ether the dinitrobeuzene is determined gravimetrically. Joachimoglu reports that this method is about 55 to 60 percent quantitative."